GPX2

Gene Symbol GPX2
Entrez Gene 2877
Alt Symbol GI-GPx, GPRP, GPRP-2, GPx-2, GPx-GI, GSHPX-GI, GSHPx-2
Species Human
Gene Type protein-coding
Description glutathione peroxidase 2
Other Description gastrointestinal glutathione peroxidase|glutathione peroxidase 2 (gastrointestinal)|glutathione peroxidase-related protein 2
Swissprots Q6PJ52 Q9NRP9 P18283 Q8WWI7
Accessions AAF74026 AAV31780 EAW80889 EAW80890 EAW80891 EAW80892 P18283 AK225871 BC005277 AAH05277 BC016756 AAH16756 BC022820 AAH22820 BC039850 BC046164 BC067221 AAH67221 BF207526 BG429974 X53463 CAB43534 X68314 CAA48394 NM_002083 NP_002074 NR_046320 NR_046321
Function Could play a major role in protecting mammals from the toxicity of ingested organic hydroperoxides. Tert-butyl hydroperoxide, cumene hydroperoxide and linoleic acid hydroperoxide but not phosphatidycholine hydroperoxide, can act as acceptors.
Subcellular Location Cytoplasm. Note=Mainly cytoplasmic.
Tissue Specificity Mostly in liver and gastrointestinal tract, not found in heart or kidney.
Top Pathways Thyroid hormone synthesis, Glutathione metabolism, Arachidonic acid metabolism

GPx-2 (N-15) - sc-54604 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Glutathione Peroxidase 2 antibody - ab137431 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Glutathione Peroxidase 2 antibody (ab137431) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

Anti-Glutathione Peroxidase 2 antibody - ab64322 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Ab64322 staining human normal bladder tissue. Staining is localized to cytoplasm.Left panel: with primary antibody diluted 1:1000. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Glutathione Peroxidase 2 antibody - ab96798 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab96798 stained CACO-2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96798 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Glutathione Peroxidase 2 antibody - ab36140 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-Glutathione Peroxidase 2 antibody (ab36140) at 1 µg/ml + Human Prostate lysate (RIPA buffer, 30µg total protein per lane).

Anti-Glutathione Peroxidase 2 antibody [EPR8175(2)] - ab140130 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8175(2)
All lanes : Anti-Glutathione Peroxidase 2 antibody [EPR8175(2)] (ab140130) at 1/1000 dilutionLane 1 : Human colon cancer lysateLane 2 : Human stomach lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-Glutathione Peroxidase 2 antibody - N-terminal - ab191030 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunocytochemical analysis of HeLa cells labeling Glutathione Peroxidase 2 with ab191030 at 1 µg/ml.

Anti-GPX2 Antibody (aa44-60) - LS-C312955 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
GPX2 antibody. ICC: HELA Cell.

Anti-GPX2 Antibody (C-Terminus) - LS-C144722 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-GPX2 Antibody (aa15-24) - LS-C54920 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody staining (1 ug/ml) of Human Prostate lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Anti-GPX2 Antibody - LS-C109298 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Sample(30 g of whole cell lysate). A: MOLT4. 15% SDS PAGE. GPX2 antibody diluted at 1:100.

Anti-GPX2 Antibody IHC-plus™ - LS-B8474 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-GPX2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8474 dilution 10 ug/ml.

Anti-GPX2 Antibody (aa92-141) - LS-C117048 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
GPX2 antibody LS-C117048 Western blot of HepG2 cell lysate.

Glutathione Peroxidase 2/GPX2 Antibody - NB100-1888 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Glutathione Peroxidase 2/GPX2 Antibody (496010) - MAB5470 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 496010
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, human liver tissue, Hepa 1‑6 mouse hepatoma cell line, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Glutathione Peroxidase 2 Monoclonal Antibody (Catalog # MAB5470) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Glutathione Peroxidase 2 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Glutathione Peroxidase 2/GPX2 Antibody - NBP1-32002 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [C1C3] diluted at 1:4000.

Glutathione Peroxidase 2/GPX2 Antibody - NBP1-30950 from Novus Biologicals

Prices $349.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: Glutathione Peroxidase 2 Antibody [NBP1-30950] - MOLT4, antibody diluted at 1:100 with 15% SDS PAGE gel.

GPX2/Glutathione Peroxidase 2 Antibody - bs-13396R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit

Rabbit Anti-GPX2/Glutathione Peroxidase 2 Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated - bs-13396R-A350 from Bioss

Prices $325.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Rabbit Anti-GPX2/Glutathione Peroxidase 2 Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated - bs-13396R-A488 from Bioss

Prices $325.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Rabbit Anti-GPX2/Glutathione Peroxidase 2 Polyclonal Antibody, ALEXA FLUOR® 555 Conjugated - bs-13396R-A555 from Bioss

Prices $325.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Rabbit Anti-GPX2/Glutathione Peroxidase 2 Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated - bs-13396R-A647 from Bioss

Prices $325.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Rabbit Anti-GPX2/Glutathione Peroxidase 2 Polyclonal Antibody, Cy3 Conjugated - bs-13396R-Cy3 from Bioss

Prices $345.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Rabbit Anti-GPX2/Glutathione Peroxidase 2 Polyclonal Antibody, Cy5 Conjugated - bs-13396R-Cy5 from Bioss

Prices $345.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Rabbit Anti-GPX2/Glutathione Peroxidase 2 Polyclonal Antibody, Cy5.5 Conjugated - bs-13396R-Cy5.5 from Bioss

Prices $345.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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