AGAP1

Gene Symbol AGAP1
Entrez Gene 116987
Alt Symbol AGAP-1, CENTG2, GGAP1, cnt-g2
Species Human
Gene Type protein-coding
Description ArfGAP with GTPase domain, ankyrin repeat and PH domain 1
Other Description Arf GAP with GTP-binding protein-like, ANK repeat and PH domains 1|GTP-binding and GTPase-activating protein 1|arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1|centaurin, gamma 2
Swissprots Q9NV93 B2RTX7 Q541S5 Q9UPQ3 Q6P9D7
Accessions AAX88852 AAX88857 AAY14697 AAY24137 EAW71078 EAW71079 Q9UPQ3 AB029022 BAA83051 AF413078 AAL04172 AK001722 BAA91862 AK295520 BAG58435 AK300763 BAG62428 AY033765 AAK56506 BC060814 AAH60814 BC140856 AAI40857 BC167153 AAI67153 BC167157 AAI67157 BI868924 BM752978 CA446024 CB961699 DQ570804 DQ584637 KJ534764 AHW56404 XM_005246059 XP_005246116 XM_006712234 XP_006712297 XM_006712235 XP_006712298 XM_006712237 XP_006712300 XM_006712239 XP_006712302 XM_011510547 XP_011508849 XM_011510548 XP_011508850 XM_011510549 XP_011508851 NM_001037131 NP_001032208 NM_001244888 NP_001231817 NM_014914 NP_055729
Function GTPase-activating protein for ARF1 and, to a lesser extent, ARF5. Directly and specifically regulates the adapter protein 3 (AP-3)-dependent trafficking of proteins in the endosomal-lysosomal system. {ECO:0000269|PubMed:12640130}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:12388557, ECO:0000269|PubMed:12640130}. Note=Associates with the endocytic compartment.
Tissue Specificity Widely expressed. {ECO:0000269|PubMed:12388557, ECO:0000269|PubMed:12640130, ECO:0000269|PubMed:15381706, ECO:0000269|PubMed:16079295}.
Top Pathways Endocytosis

AGAP1 (N-15) - sc-47786 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
AGAP1 (N-15): sc-47786. Western blot analysis of AGAP1 expression in NIH/3T3 (A), SW480 (B) and Jurkat (C) whole cell lysates.

AGAP1/3 (H-63) - sc-367637 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Centaurin γ3 (E-11) - sc-390362 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Centaurin Y3 (E-11): sc-390362. Western blot analysis of Centaurin Y3 expression in human hippocampus tissue extract.

Centaurin γ3 (M-18) - sc-47832 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Centaurin γ3 (M-18): sc-47832. Western blot analysis of Centaurin γ3 expression in non-transfected 293T: sc-117752 (A), mouse Centaurin γ3 transfected 293T: sc-119171 (B) and HeLa (C) whole cell lysates.

Anti-AGAP1 antibody - ab199136 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-AGAP1 antibody (ab199136) at 1/400 dilutionLane 1 : Human liver cancer tissue lysateLane 2 : HeLa cell lysateLane 3 : Mouse lung tissue lysateLysates/proteins at 40 µg per lane.developed using the ECL technique

Anti-AGAP1 antibody - ab151480 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-AGAP1 antibody (ab151480) at 1/1000 dilution + MCF-7 whole cell lysate at 30 µg

Anti-AGAP1 antibody - ab96827 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-AGAP1 antibody (ab96827) at 1/1000 dilution + Hep G2 whole cell lysate at 30 µg

Anti-AGAP1 antibody - ab105329 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-AGAP1 antibody (ab105329) at 1/1000 dilutionLane 1 : Human fetal heart lysateLane 2 : Human fetal kidney lysate

Anti-AGAP1 antibody - ab77031 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-AGAP1 antibody (ab77031) at 1/500 dilutionLane 1 : Lysate from 293T cells, transfected with AGAP1 (392aa isoform)Lane 2 : Lysate from non-transfected 293T cellsSecondaryGoat Anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution

Anti-CENTG2 / AGAP1 Antibody (C-Terminus) - LS-C54642 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-CENTG2 / AGAP1 Antibody - LS-C307782 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-CENTG2 / AGAP1 Antibody (aa302-351) - LS-C187258 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
CENTG2 / AGAP1 antibody Western Blot of NCI-H226. Antibody dilution: 1 ug/ml.

Anti-CENTG2 / AGAP1 Antibody (aa795-857) - LS-C185784 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescence of paraformaldehyde-fixed HeLa using CENTG2 antibody at 1:200 dilution.

Anti-CENTG2 / AGAP1 Antibody (clone 4E8) - LS-C173696 from LifeSpan Bioscience

Prices $350.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4E8
IHC of paraffin-embedded Human tonsil using anti-AGAP1 mouse monoclonal antibody.

AGAP1 Antibody (4E8) - NBP2-01708 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4E8
Western Blot: AGAP1 Antibody (4E8) [NBP2-01708] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AGAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AGAP1.

AGAP1 Antibody - NBP1-31261 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: AGAP1 Antibody [NBP1-31261] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE; antibody diluted at 1:1000.

AGAP1 Antibody - NBP2-15305 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: AGAP1 Antibody [NBP2-15305] - Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE; antibody diluted at 1:1000.

AGAP1 Antibody - NB100-1282 from Novus Biologicals

Prices $229.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: AGAP1 Antibody [NB100-1282] - NB100-1282 staining (0.25ug/ml) of 293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

AGAP1 Antibody - H00116987-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

AGAP1 Antibody (3F2) - H00116987-M01-100ug from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3F2

Anti-AGAP1 antibody produced in rabbit - HPA051405 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-AGAP1: <B>Cat. No. HPA051405</B>: Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.

Anti-AGAP1 (C-terminal) antibody produced in rabbit - SAB2700913 from Sigma-Aldrich

Prices $326.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-AGAP1 (C-terminal): <B>Cat. No. SAB2700913</B>: Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CENTG2 antibody at 1:200 dilution.

Anti-CENTG2 antibody produced in mouse - SAB1408256 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-CENTG2: <B>Cat. No. SAB1408256</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~43.1 kDa using AGAP1 transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-AGAP1 antibody produced in rabbit - SAB2701279 from Sigma-Aldrich

Prices $326.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-AGAP1: <B>Cat. No. SAB2701279</B>: Immunohistochemical analysis of paraffin-embedded MDAMB231 xenograft, using Centaurin gamma 2 antibody at 1:250 dilution.

Anti-AGAP1 (381-395) antibody produced in rabbit - SAB1104225 from Sigma-Aldrich

Prices $285.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human AGAP1 was separated on SDS-PAGE, blotted with Anti-AGAP1 (381-395) (<B>Cat. No. SAB1104225</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:1,000
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