Gene Symbol | BRAP |
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Entrez Gene | 8315 |
Alt Symbol | BRAP2, IMP, RNF52 |
Species | Human |
Gene Type | protein-coding |
Description | BRCA1 associated protein |
Other Description | BRCA1-associated protein|RING finger protein 52|galectin-2-binding protein|impedes mitogenic signal propagation|renal carcinoma antigen NY-REN-63 |
Swissprots | O43238 B4DRM1 O75341 Q7Z569 B9EGS8 |
Accessions | EAW97972 Q7Z569 AB208878 BAD92115 AF035620 AAC24200 AF035950 AAB88538 AK290379 BAF83068 AK299326 BAG61333 AK308279 AY332222 AAP93638 BC136698 AAI36699 BC136699 AAI36700 BU674276 DB460078 XM_005253944 XP_005254001 XM_011538788 XP_011537090 XM_011538789 XP_011537091 NM_006768 NP_006759 |
Function | Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto- polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport. {ECO:0000269|PubMed:14724641, ECO:0000303|PubMed:10777491}. |
Subcellular Location | Cytoplasm {ECO:0000269|PubMed:9497340}. |
Tissue Specificity | Expressed in breast epithelial cell lines. {ECO:0000269|PubMed:9497340}. |
BRAP CRISPR/Cas9 KO Plasmid (h) - sc-403847 from Santa Cruz Biotechnology
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BRAP HDR Plasmid (h) - sc-403847-HDR from Santa Cruz Biotechnology
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BRAP Double Nickase Plasmid (h) - sc-403847-NIC from Santa Cruz Biotechnology
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BRAP Double Nickase Plasmid (h2) - sc-403847-NIC-2 from Santa Cruz Biotechnology
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BRAP CRISPR Activation Plasmid (h) - sc-403847-ACT from Santa Cruz Biotechnology
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BRAP CRISPR Activation Plasmid (h2) - sc-403847-ACT-2 from Santa Cruz Biotechnology
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BRAP Lentiviral Activation Particles (h) - sc-403847-LAC from Santa Cruz Biotechnology
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BRAP Lentiviral Activation Particles (h2) - sc-403847-LAC-2 from Santa Cruz Biotechnology
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BRAP siRNA (h) - sc-95995 from Santa Cruz Biotechnology
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BRAP shRNA Plasmid (h) - sc-95995-SH from Santa Cruz Biotechnology
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BRAP shRNA (h) Lentiviral Particles - sc-95995-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU020871 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN4045 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND4045 from Sigma-Aldrich
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BRAP siRNA (Human) - CRH5439 from Cohesion Biosciences
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BRAP RNAi - H00008315-R01 from Novus Biologicals
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BRAP - MBS8210698 from MyBioSource
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shRNA set against Human BRAP (NM_006768.3) - SHH247534 from Creative biogene
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BRAP shRNA Plasmid (Human) - CHH5439 from Cohesion Biosciences
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