CLINT1

Gene Symbol CLINT1
Entrez Gene 9685
Alt Symbol CLINT, ENTH, EPN4, EPNR
Species Human
Gene Type protein-coding
Description clathrin interactor 1
Other Description clathrin interacting protein localized in the trans-Golgi region|enthoprotin|epsin 4|epsin-related protein|epsinR
Swissprots Q96E05 Q8NAF1 D3DQJ6 B7Z6F8 Q14677
Accessions CCQ43453 EAW61585 EAW61586 EAW61587 EAW61588 Q14677 AF434813 AAL30768 AK092765 BAC03971 AK128594 AK300257 BAH13244 AL529948 AL833548 BC004467 AAH04467 BC013091 AAH13091 BC030264 BI461076 BK000414 DAA00062 BP246338 D79993 BAA11488 XM_011534701 XP_011533003 XM_011534702 XP_011533004 XM_011534703 XP_011533005 NM_001195555 NP_001182484 NM_001195556 NP_001182485 NM_014666 NP_055481
Function Binds to membranes enriched in phosphatidylinositol 4,5- bisphosphate (PtdIns(4,5)P2). May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. Stimulates clathrin assembly. {ECO:0000269|PubMed:12429846, ECO:0000269|PubMed:12538641}.
Subcellular Location Cytoplasm. Cytoplasm, perinuclear region. Membrane; Peripheral membrane protein. Cytoplasmic vesicle, clathrin-coated vesicle. Note=Found throughout the cell, with the exception of the cell surface. Concentrated in the perinuclear region and associated with clathrin-coated vesicles close to the trans-Golgi network.
Tissue Specificity Ubiquitously expressed at low to intermediate levels. {ECO:0000269|PubMed:12429846, ECO:0000269|PubMed:12538641}.

epsin 4 (C-18) - sc-51361 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

epsin 4 (D-19) - sc-51362 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

epsin 4 (N-20) - sc-51363 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

epsin 4 (S-16) - sc-51364 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-CLINT1 antibody - ab76482 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-CLINT1 antibody (ab76482) at 5 µg/ml + recombinant CLINT1 fragment with 26 kDa tag at 0.2 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

Anti-CLINT1 Antibody (aa1-290, clone 8C1) - LS-C339789 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 8C1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLINT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLINT1.

Anti-CLINT1 Antibody (aa1-290, clone 6G10) - LS-C339792 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6G10
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLINT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLINT1.

Anti-CLINT1 Antibody (aa1-290, clone 9E1) - LS-C339791 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 9E1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLINT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLINT1.

Anti-CLINT1 Antibody (aa1-290, clone 3H9) - LS-C339793 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3H9
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLINT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLINT1.

Anti-CLINT1 Antibody (aa229-278) - LS-C145831 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
CLINT1 antibody LS-C145831 Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml.

Anti-CLINT1 Antibody (aa1-290, clone 10F12) - LS-C339804 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 10F12
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLINT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLINT1.

Anti-CLINT1 Antibody (aa1-290, clone 8B6) - LS-C339794 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 8B6
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLINT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLINT1.

Anti-CLINT1 Antibody (aa228-258) - LS-C167770 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
(LEFT)Formalin-fixed and paraffin-embedded human brain with ENTH Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. (RIGHT)Flow cytometric of NCI-H292 cells using ENTH Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Anti-CLINT1 Antibody (clone 1E6) - LS-C134005 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1E6
Immunoperoxidase of monoclonal antibody to CLINT1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]

Anti-CLINT1 Antibody - LS-C128796 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CLINT1 Antibody (aa150-200) - LS-C288695 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CLINT1 Antibody (aa250-300) - LS-C288701 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CLINT1 Antibody (aa108-157) - LS-C145830 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
CLINT1 antibody LS-C145830 Western blot of Fetal Lung lysate. Antibody concentration 1 ug/ml.

Clathrin interactor 1 Antibody (1E6) - H00009685-M03 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1E6
Western Blot: Clathrin interactor 1 Antibody (1E6) [H00009685-M03] - Analysis of ENTH expression in Jurkat (Cat # L017V1).

Clathrin interactor 1 Antibody - NBP1-85570 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Clathrin interactor 1 Antibody [NBP1-85570] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Clathrin interactor 1 Antibody - NBP1-05991 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Clathrin interactor 1 Antibody [NBP1-05991] - Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells. EpsinR was also immunoprecipitated by rabbit anti-EpsinR antibody NBP1-05990.

Clathrin interactor 1 Antibody - NBP1-05990 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Clathrin interactor 1 Antibody [NBP1-05990] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EpsinR antibody used for IP at 3 mcg/mg lysate.

Monoclonal Anti-ENTH antibody produced in mouse - SAB1400360 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1E6
<B>Immunoblotting</B><BR/>Monoclonal Anti-ENTH, antibody produced in mouse, clone 1E6, purified immunoglobulin: <B>Cat. No. SAB1400360</B>: Antibody concentration: 1.0 ~ 5.0 μg/mL. Specific band of ~68.3 kDa using Jurkat lysate.

Anti-CLINT1 antibody produced in rabbit - HPA043280 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-CLINT1: <B>Cat. No. HPA043280</B>: Immunohistochemical staining of human colon shows strong granular cytoplasmic positivity in glandular cells.

Anti-CLINT1 (171-185) antibody produced in rabbit - SAB1100661 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-CLINT1 (171-185) : <B>Cat. No. SAB1100661</B>: Immunofluorescence of HUVEC cells using CLINT1 (171-185) , Cat. No. SAB1100661 (red) at a 1:200 dilution, taken at 40x magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com
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