BCKDHA

Gene Symbol BCKDHA
Entrez Gene 593
Alt Symbol BCKDE1A, MSU, MSUD1, OVD1A
Species Human
Gene Type protein-coding
Description branched chain keto acid dehydrogenase E1, alpha polypeptide
Other Description 2-oxoisovalerate dehydrogenase (lipoamide)|2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial|BCKDH E1-alpha|branched-chain alpha-keto acid dehydrogenase E1 component alpha chain
Swissprots P12694 Q16472 B4DP47 Q16034 E7EW46
Accessions AAB19267 EAW57039 EAW57040 P12694 AB209828 BAD93065 AK298188 BAG60459 AK313300 BAG36106 AL833143 CAH56215 BC007878 AAH07878 BC008933 AAH08933 BC023983 AAH23983 BE223026 BG742673 BI910860 BM702667 BQ018849 J04474 AAB59549 M22221 AAA35590 Z14093 CAA78475 NM_000709 NP_000700 NM_001164783 NP_001158255
Function The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).
Subcellular Location Mitochondrion matrix.
Top Pathways Valine, leucine and isoleucine degradation

BCKDE1A (H-5) - sc-271538 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
BCKDE1A (H-5): sc-271538. Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.

BCKDE1A (H-300) - sc-67200 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
BCKDE1A (H-300): sc-67200. Western blot analysis of BCKDE1A expression in non-transfected 293T: sc-117752 (A), mouse BCKDE1A transfected 293T: sc-126497 (B) and HeLa (C) whole cell lysates.

BCKDE1A (G-18) - sc-51471 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
BCKDE1A (G-18): sc-51471. Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes.

Anti-BCKDHA antibody - ab126173 from Abcam

Prices $384.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-BCKDHA antibody (ab126173) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

Anti-BCKDHA antibody - ab138460 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-BCKDHA antibody (ab138460) at 1/2000 dilutionLane 1 : HeLa cell lysate at 50 µgLane 2 : HeLa cell lysate at 15 µgLane 3 : 293T cell lysate at 50 µgLane 4 : Jurkat cell lysate at 50 µgdeveloped using the ECL technique

Anti-BCKDHA antibody - ab68094 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-BCKDHA antibody (ab68094) at 1/500 dilutionLane 1 : BCKDHA transfected 293T cell lysateLane 2 : non transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-BCKDHA antibody - ab137244 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunoprecipitation with ab137244 at 6µg/mg lysate. 1 mg of HeLa whole cell lysate (20% of IP loaded). Subsequent WB detection used an anti-BCKDHA antibody which recognizes a downstream epitope at 1 µg/ml. Detected by chemiluminescence with exposure time of 30 seconds.

Anti-BCKDHA / BCKDE1A Antibody (aa71-120) - LS-C111808 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
BCKDHA antibody LS-C111808 Western blot of MCF7 cell lysate.

Anti-BCKDHA / BCKDE1A Antibody (aa75-125) - LS-C290442 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-BCKDHA / BCKDE1A Antibody (aa395-445) - LS-C290444 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-BCKDHA / BCKDE1A Antibody - LS-C306521 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-BCKDHA / BCKDE1A Antibody (aa362-390) - LS-C156765 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with BCKDHA Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-BCKDHA / BCKDE1A Antibody - LS-C155321 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded Colon ca, using BCKDHA antibody at 1:250 dilution.

BCKDHA Antibody - NBP1-79616 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: BCKDHA Antibody [NBP1-79616] - MCF-7 whole cell lysates, concentration 0.2-1 ug/ml.

BCKDHA Antibody - NBP2-15552 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: BCKDHA Antibody [NBP2-15552] - Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE gel, diluted at 1:1000.

BCKDHA Antibody - NBP1-81022 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

BCKDHA Antibody - H00000593-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

BCKDHA Antibody - H00000593-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: BCKDHA Antibody [H00000593-B01] - analysis of BCKDHA expression in transfected 293T cell line by BCKDHA MaxPab polyclonal antibody (B01). Lane 1: BCKDHA transfected lysate (50.50 KDa). Lane 2: Non-transfected lysate.

BCKDHA Antibody - H00000593-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: BCKDHA Antibody [H00000593-A01] - BCKDHA polyclonal antibody (A01), Lot # 051212JC01 Western Blot analysis of BCKDHA expression in Y-79 ( Cat # L042V1 ).

Anti-BCKDHA antibody produced in rabbit - HPA036640 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-BCKDHA: <B>Cat. No. HPA036640</B>: Immunohistochemical staining of human kidney shows strong granular positivity in cells in tubules.

Anti-BCKDHA antibody produced in rabbit - SAB2702057 from Sigma-Aldrich

Prices $326.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-BCKDHA: <B>Cat. No. SAB2702057</B>: Immunohistochemical analysis of paraffin-embedded colon carcinoma antibody at 1:250 dilution.

Anti-BCKDHA antibody produced in mouse - SAB1405509 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-BCKDHA: <B>Cat. No. SAB1405509</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~49 kDa using BCKDHA transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-BCKDHA, (N-terminal) antibody produced in rabbit - SAB2104058 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-BCKDHA, antibody produced in rabbit: <B>Cat. No. SAB2104058</B>: Immunoblotting analysis of BCKDHA in MCF7 cell lysate with BCKDHA anitbody at 1 μg/mL.

BCKDHA antibody - N-terminal region (ARP56119_P050) - ARP56119_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

BCKDHA-Antibody-C-term - AP6830b from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
Western blot analysis of BCKDHA Antibody (C-term) (Cat. #AP6830b) in Jurkat cell line lysates (35ug/lane). BCKDHA (arrow) was detected using the purified Pab.
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