RPTOR

Gene Symbol	RPTOR
Entrez Gene	57521
Alt Symbol	KOG1, Mip1
Species	Human
Gene Type	protein-coding
Description	regulatory associated protein of MTOR, complex 1
Other Description	p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats\|raptor\|regulatory-associated protein of mTOR
Swissprots	Q8TB32 C6KEF2 B2RN36 Q9P2P3 F5H7J5 Q8N4V9 Q8N122
Accessions	EAW89618 EAW89619 Q8N122 AB037724 BAA92541 AB082951 BAC06490 AI821182 AK055912 AK295964 BAG58742 AK296321 BAH12314 AY090663 AAM09075 BC014502 BC025180 AAH25180 BC033258 AAH33258 BC033699 BC041079 BC064515 AAH64515 BC073898 AAH73898 BC136652 AAI36653 BC136654 AAI36655 BF111734 GQ183898 ACS44766 NM_001163034 NP_001156506 NM_020761 NP_065812
Function	Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylati
Subcellular Location	Cytoplasm. Lysosome. Cytoplasmic granule. Note=Targeting to lysosomes depends on amino acid availability. In arsenite-stressed cells, accumulates in stress granules when associated with SPAG5 and association with lysosomes is drastically decreased.
Tissue Specificity	Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. Isoform 3 is widely expressed, with highest levels in nasal mucosa and pituitary and lowest in spleen. {ECO:0000269\|PubMed:12150925, ECO:0000269\|PubMed:12408816}.
Top Pathways	Insulin signaling pathway, PI3K-Akt signaling pathway, MicroRNAs in cancer, AMPK signaling pathway, mTOR signaling pathway

Phospho-Raptor (Ser792) Antibody - 2083 from Cell Signaling Technology

Prices	$109.00, $287.00
Sizes	20 µl (2 western blots), 100 µl (10 western blots)
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of C2C12 or 293 cells, untreated or treated with AICAR (0.5 mM for 30 minutes) or oligomycin (0.5 μM for 30 minutes), using Phospho-Raptor (Ser792) Antibody (upper and lower left ) or Raptor Antibody #2280 (upper and lower right).

Raptor (24C12) Rabbit mAb - 2280 from Cell Signaling Technology

Prices	$99.00, $246.00
Sizes	20 µl (2 western blots), 100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	24C12

Western blot analysis of extracts from various cell lines, using Raptor (24C12) Rabbit mAb.

Raptor Antibody - 4978 from Cell Signaling Technology

Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts from HeLa and 293 cells using Raptor Antibody.

Raptor (W990) Antibody - 2214 from Cell Signaling Technology

Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells using Raptor (W990) Antibody.

Raptor (N-19) - sc-27744 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Raptor (N-19): sc-27744. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebral cortex tissue showing cytoplasmic staining of hematopoietic cells, glial cells and endothelial cells.

Raptor (10E10) - sc-81537 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	50 µg/0.5 ml
Host	Mouse
Clonality	Monoclonal

Raptor (10E10): sc-81537. Western blot analysis of Raptor expression in serum starved HeLa (A), Hep G2 (B), HEK293 (C), SH-SY5Y (D), MDCK (E), PC-12 (F), CMT 93 (G), Neuro 2A (H) and 3T3 (I) whole cell lysates.

p-Raptor (Ser 863) - sc-130214 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	100 µg/ml
Host	Rabbit
Clonality	Polyclonal

Anti-Raptor antibody - ab5454 from Abcam

Prices	$391.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-Raptor antibody - ab62454 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Lane 1 : Anti-Raptor antibody (ab62454) at 2 µg/mlLane 2 : Anti-Raptor antibody (ab62454) at 4 µg/mlLane 1 : L1210 cell lysateLane 2 : L1210 cell lysateLysates/proteins at 15 µg per lane.

Anti-Raptor antibody - ab26264 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Lane 1 : Anti-Raptor antibody (ab26264) at 2 µg/mlLane 2 : Anti-Raptor antibody (ab26264) at 4 µg/mlLane 1 : L1210 cell lysateLane 2 : L1210 cell lysate

Anti-Raptor antibody - ab169506 from Abcam

Prices	$376.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

All lanes : Anti-Raptor antibody (ab169506) at 1 µg/mlLane 1 : Raptor transfected 293T lysateLane 2 : Non-transfected 293T lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Anti-Raptor antibody - ab189158 from Abcam

Prices	$376.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-Raptor antibody (ab189158) at 1/1000 dilutionLane 1 : Human 293 cell lysateLane 2 : Mouse NIH 3T3 cell lysate

Anti-Raptor antibody - ab125351 from Abcam

Prices	$376.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-Raptor antibody (ab125351) at 1/250 dilutionLane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 4 : PANC-1 (Human Pancreatic Carcinoma) Whole Cell Lysate Lane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Raptor antibody - ab70212 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-Raptor antibody [1411CT316215134] - ab202319 from Abcam

Prices	$359.00
Sizes	400 µl
Host	Mouse
Clonality	Monoclonal
Clone	1411CT316215134

All lanes : Anti-Raptor antibody [1411CT316215134] (ab202319) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : MCF7 cell lysateLane 3 : C2C12 cell lysateLane 4 : NIH 3T3 cell lysateLane 5 : C6 cell lysateLysates/proteins at 20 µg per lane.Secondarygoat anti-rabbit IgG H&L(HRP) at 1/10000 dilution

Anti-Raptor antibody [EP539Y] - ab40768 from Abcam

Prices	$394.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EP539Y

All lanes : Anti-Raptor antibody [EP539Y] (ab40768) at 1/500 dilutionLane 1 : 293 cell lysateLane 2 : MCF-7 cell lysateLane 3 : HeLa cell lysateLane 4 : A431 cell lysateLysates/proteins at 10 µg per lane.

Anti-Raptor / Mip1 Antibody - LS-C38752 from LifeSpan Bioscience

Prices	$455.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-Raptor / Mip1 Antibody (N-Terminus) - LS-C75333 from LifeSpan Bioscience

Prices	$455.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-Raptor / Mip1 Antibody (aa975-1025) - LS-C93663 from LifeSpan Bioscience

Prices	$635.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-Raptor / Mip1 Antibody (phospho-Ser706) - LS-C131800 from LifeSpan Bioscience

Prices	$445.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-Raptor / Mip1 Antibody (phospho-Ser706) - LS-C131801 from LifeSpan Bioscience

Prices	$445.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-Raptor / Mip1 Antibody (phospho-Ser877) - LS-C131802 from LifeSpan Bioscience

Prices	$445.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-Raptor / Mip1 Antibody - LS-C131803 from LifeSpan Bioscience

Prices	$445.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

Anti-Raptor / Mip1 Antibody (phospho-Ser863) - LS-C96828 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

Dot blot of anti-raptor-pS863 antibody (RB13350) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Anti-Raptor / Mip1 Antibody - LS-C43066 from LifeSpan Bioscience

Prices	$350.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Detection of Raptor in whole cell lysates of rat L6 (1) mouse C2C12 (2) and Human HEK293 (3) cells by Western blotting using Rabbit anti-Human Raptor (LS-C43066).

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