SNRPF

Gene Symbol	SNRPF
Entrez Gene	6636
Alt Symbol	SMF, Sm-F, snRNP-F
Species	Human
Gene Type	protein-coding
Description	small nuclear ribonucleoprotein polypeptide F
Other Description	sm protein F\|small nuclear ribonucleoprotein F
Swissprots	B2R498 Q6IBQ1 Q6P4I0 P62306 A2VCR2
Accessions	EAW97548 EAW97549 P62306 AK311752 BAG34695 BC002505 AAH02505 BC063397 AAH63397 BC128452 AAI28453 BC128453 AAI28454 BM972965 BU861031 CR456751 CAG33032 X85372 CAA59688 NM_003095 NP_003086
Function	Core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in an heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. As part of the U7 snRNP it is involved in histone 3'-end processing. {ECO:0000269\|PubMed:18984161, ECO:0000269\|PubMed:23333303}.
Subcellular Location	Cytoplasm, cytosol {ECO:0000269\|PubMed:18984161}. Nucleus {ECO:0000269\|PubMed:18984161}. Note=SMN-mediated assembly into core snRNPs occurs in the cytosol before SMN-mediated transport to the nucleus to be included in spliceosomes.
Top Pathways	Spliceosome

Sm F (D-16) - sc-21060 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Sm F (D-16): sc-21060. Western blot analysis of Sm F expression in non-transfected: sc-117750 (A) and human Sm F transfected: sc-112407 (B) whole cell lysates.

Sm F (N-13) - sc-21059 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Sm F (N-13): sc-21059. Western blot analysis of Sm F expression in HL-60 whole cell lysate (A) and HeLa (B), Jurkat (C), K-562 (D) and NIH/3T3 (E) nuclear extracts.

Anti-SNRPF antibody [EPR10388] - ab154870 from Abcam

Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR10388

All lanes : Anti-SNRPF antibody [EPR10388] (ab154870) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : MCF-7 cell lysateLane 3 : HeLa cell lysateLane 4 : HL-60 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-SNRPF antibody [EPR10389(B)] - ab156587 from Abcam

Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR10389(B)

All lanes : Anti-SNRPF antibody [EPR10389(B)] (ab156587) at 1/1000 dilutionLane 1 : C6 cell lysatesLane 2 : MCF7 cell lysateLane 3 : PC12 cell lysate Lane 4 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-SNRPF Antibody (C-Terminus) - LS-C80333 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

MCF7 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 10-20%.

Anti-SNRPF Antibody (N-Terminus) - LS-C345729 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of RUXF Antibody with human 293T Whole Cell lysate.

Anti-SNRPF Antibody (N-Terminus) - LS-C30552 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

SNRPF antibody LS-C30552 was used in IHC to stain formalin-fixed, paraffin-embedded human heart.

SNRPF Antibody - NBP1-57463 from Novus Biologicals

Prices	$139.00, $299.00
Sizes	20 µl, 100 µl
Host	Rabbit
Clonality	Polyclonal

SNRPF Antibody - NBP1-57464 from Novus Biologicals

Prices	$139.00, $329.00
Sizes	20 µl, 100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-HMGXB3 antibody produced in rabbit - HPA005967 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-HMGXB3: <B>Cat. No. HPA005967</B>: Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in a subset of leukocytes.

Anti-SNRPF antibody produced in rabbit - SAB2102258 from Sigma-Aldrich

Prices	$347.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-SNRPF: <B>Cat. No.SAB2102258</B>: SNRPF in MCF7 cell lysate with SNRPF antibody at 1.0 μg/mL.

Anti-SNRPF antibody produced in rabbit - AV40447 from Sigma-Aldrich

Prices	$267.50
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-SNRPF: <B>Cat. No. AV40447</B>: Immunohistochemistry of SNRPF in Human Heart tissue with SNRPF antibody at 4-8 μg/mL.

SNRPF antibody - N-terminal region (ARP40447_T100) - ARP40447_T100 from Aviva Systems Biology

Prices	$229.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

SNRPF antibody - middle region (ARP40448_P050) - ARP40448_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

RUXF Antibody - N-terminal region (ARP74249_P050) - ARP74249_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

SNRPF antibody - 70R-20432 from Fitzgerald

Prices	$375.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

SNRPF Polyclonal Antibody - A4121 from ABclonal

Prices	$200.00, $480.00
Sizes	50 µg, 200 µg
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts of HepG2 cell line, using SNRPF antibody.

SNRPF antibody - 70R-1423 from Fitzgerald

Prices	$275.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

SNRPF Antibody - abx003036 from Abbexa

Host	Rabbit
Clonality	Polyclonal

snRNP-F / Sm-F antibody - 14977-1-AP from Acris Antibodies

Prices	$270.00
Sizes	150 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry of paraffin-embedded Breast cancer using 14977-1-AP(SNRPF Antibody) at Dilution 1:100 (under 10x lens)

SNRPF Antibody - 29-304 from ProSci

Prices	$250.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Antibody used in WB on Human HepG2 at 1 ug/ml.

SNRPF antibody - STJ25644 from St John's Laboratory Ltd

Host	Rabbit
Clonality	Polyclonal

SNRPF antibody - 14977-1-AP from Proteintech Group

Prices	$279.00
Sizes	con: 23 μg/150μl
Host	Rabbit
Clonality	Polyclonal

human heart tissue were subjected to SDS PAGE followed by western blot with 14977-1-AP(SNRPF antibody) at dilution of 1:500

SNRPF - MBS129453 from MyBioSource

Prices	$285.00, $555.00
Sizes	50 µg, 200 µg
Host	Rabbit
Clonality	Polyclonal

SNRPF - A4121 from NeoScientific

Prices	$480.00
Sizes	200 µg
Host	Rabbit
Clonality	Polyclonal

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