GRK5

Gene Symbol GRK5
Entrez Gene 2869
Alt Symbol GPRK5
Species Human
Gene Type protein-coding
Description G protein-coupled receptor kinase 5
Other Description FP2025|g protein-coupled receptor kinase GRK5
Swissprots Q5T059 D3DRD0 P34947
Accessions CBX53840 EAW49393 EAW49394 EAW49395 P34947 AF370369 AAQ15205 AK097099 AK298261 BAG60525 AK301450 BAG62974 AK309761 AK313012 BAG35847 BC018116 BC064506 AAH64506 BC114448 AAI14449 BQ184611 CN290123 L15388 AAA58620 XM_005269707 XP_005269764 XM_005269708 XP_005269765 XM_011539699 XP_011538001 NM_005308 NP_005299
Function Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein- coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70- interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2- mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of
Subcellular Location Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Note=Predominantly localized at the plasma membrane; targeted to the cell surface through the interaction with phospholipids. Nucleus localization is regulated in a GPCR and Ca(2+)/calmodulin-dependent fashion.
Tissue Specificity Highest levels in heart, placenta, lung > skeletal muscle > brain, liver, pancreas > kidney. {ECO:0000269|PubMed:7685906}.
Top Pathways Endocytosis, Chemokine signaling pathway, Morphine addiction

GRK 5 (C-20) - sc-565 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GRK 5 (C-20): sc-565. Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing cytoplasmic and nuclear staining of macrophages and peumocytes cells.

GRK 5 (H-64) - sc-11396 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GRK 5 (H-64): sc-11396. Immunoperoxidase staining of formalin fixed, paraffin-embedded human lower stomach tissue showing cytoplasmic staining of glandular cells.

Anti-GRK5 antibody - ab64943 from Abcam

Prices $394.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab64943 staining GRK5 in Rat H9C2 cells by ICC/IF (Immunocytochemistry/immunofluorescence). H9C2 cells were infected with Ad and were treated with GRK5 and PFA fro 24 hours. Cells were fixed with paraformaldehyde, permeabilized with 1% Triton X-100 and blocked with 5% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/200 in 5% Goat serum) for 2 hours. An undiluted Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview

Anti-GRK5 antibody - ab61415 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab61415, at 6&microg/ml, staining human GRK5 in myocytes by immunohistochemistry. Formalin-fixed paraffin-embedded tissue.

Anti-GRK5 antibody [EP2045Y] - ab68429 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2045Y
Anti-GRK5 antibody [EP2045Y] (ab68429) at 1/500 dilution + Human placenta cell lysate at 10 µgSecondaryHRP labelled Goat anti-Rabbit antibody at 1/2000 dilution

Anti-GRK5 antibody - C-terminal - ab70877 from Abcam

Prices $378.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-GRK5 antibody - C-terminal (ab70877) at 1/500 dilution + HeLa cell lysate at 12.5 µg

Anti-GRK5 Antibody (aa290-339) - LS-C81918 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-GRK5 Antibody (aa351-400) - LS-C118866 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from HeLa/HepG2/HUVEC cells, using GRK5 Antibody. The lane on the right is treated with the synthesized peptide.

Anti-GRK5 Antibody (C-Terminus, clone EP2045Y) - LS-C105308 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2045Y
Western blot analysis on Human placenta cell lysate using anti-GRK5 antibody (LS-C105308), dilution 1:500.

Anti-GRK5 Antibody (clone 1D9) - LS-C197338 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1D9

Anti-GRK5 Antibody (C-Terminus, AP) - LS-C219650 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GRK5 Antibody (C-Terminus, Biotin) - LS-C219652 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GRK5 Antibody (C-Terminus, APC) - LS-C219651 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GRK5 Antibody (Gln382) - LS-C176749 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of GRK5 (Q382) pAb in extracts from HeLa cells.

Anti-GRK5 Antibody (C-Terminus, FITC) - LS-C219653 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GRK5 Antibody (C-Terminus, Azide-free, HRP) - LS-C219654 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GRK5 Antibody (C-Terminus, PE) - LS-C219656 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GRK5 Antibody (aa188-507) - LS-C311597 from LifeSpan Bioscience

Prices $250.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-GRK5 Antibody (aa1-221) - LS-C311801 from LifeSpan Bioscience

Prices $250.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-GRK5 Antibody (C-Terminus) - LS-C327491 from LifeSpan Bioscience

Prices $425.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GRK5 Antibody (Internal) IHC-plus™ - LS-A3034 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-GRK5 antibody LS-A3034 IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-GRK5 Antibody (aa369-418) - LS-C201636 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of Human 721_B. GRK5 antibody dilution 1.0 ug/ml.

Anti-GRK5 Antibody (N-Terminus) IHC-plus™ - LS-A3470 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-GRK5 antibody LS-A3470 IHC of human myocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-GRK5 Antibody (N-Terminus) IHC-plus™ - LS-A3400 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-GRK5 antibody LS-A3400 IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-GRK5 Antibody (Internal) IHC-plus™ - LS-A3030 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-GRK5 antibody LS-A3030 IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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