BARD1

Gene Symbol BARD1
Entrez Gene 580
Alt Symbol -
Species Human
Gene Type protein-coding
Description BRCA1 associated RING domain 1
Other Description BRCA1-associated RING domain gene 1|BRCA1-associated RING domain protein 1
Swissprots F6MDH8 F6MDH7 Q99728 Q53SS5 O43574 F6MDH9
Accessions AAB99978 AAX93130 EAW70519 Q99728 AK223409 BAD97129 AK301843 BAG63286 AK310759 AK314260 BAG36925 AX794378 CAE48237 BC126426 AAI26427 BC126428 AAI26429 BU677525 BU753556 BX098964 DB088853 JF790280 AEF57471 JF790281 AEF57472 JF790282 AEF57473 JF790283 AEF57474 JF790284 AEF57475 JF790285 AEF57476 JF790286 AEF57477 JF790287 AEF57478 U76638 AAB38316 XM_011511567 XP_011509869 XM_011511568 XP_011509870 NM_000465 NP_000456 NM_001282543 NP_001269472 NM_001282545 NP_001269474 NM_001282548 NP_001269477 NM_001282549 NP_001269478 NR_104212 NR_104215 NR_104216
Function Probable E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage. {ECO:0000269|PubMed:12890688, ECO:0000269|PubMed:14976165, ECO:0000269|PubMed:20351172}.
Subcellular Location Nucleus. Note=During S phase of the cell cycle, colocalizes with BRCA1 into discrete subnuclear foci. Can translocate to the cytoplasm. Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by the BRCA1-A complex.

BARD1 (E-11) - sc-74559 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
BARD1 (E-11): sc-74559. Western blot analysis of BARD1 expression in U-2 OS (A), BT-20 (B) and MCF7 (C) whole cell lysates.

BARD1 (H-300) - sc-11438 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
BARD1 (H-300): sc-11438. Western blot analysis of BARD1 expression in U-2 OS whole cell lysate.

BARD1 (N-19) - sc-7373 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
BARD1 (N-19): sc-7373. Western blot analysis of BARD1 expression in MDA-MB-231 (A), MDA-MB-468 (B) and A-431 (C) nuclear extracts.

BARD1 (C-20) - sc-7372 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-BARD1 antibody - ab64164 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-BARD1 antibody (ab64164) at 2 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-BARD1 antibody - ab115477 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab115477, at 10µg/ml, staining BARD1 in formalin-fixed, paraffin-embedded Human skeletal muscle tissue by Immunohistochemistry, using a biotinylated anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-BARD1 antibody - ab69645 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-BARD1 antibody (ab69645) at 1/500 dilutionLane 1 : HeLa cell extractLane 2 : HeLa cell extract with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-BARD1 antibody [2059C4a] - ab50984 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2059C4a
Anti-BARD1 antibody [2059C4a] (ab50984) at 1/50 dilution + HEK293 whole cell lysate at 50 µgSecondaryAnti mouse IgG antibody at 1/2500 dilution

Anti-BARD1 antibody - C-terminal - ab135055 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-BARD1 antibody - C-terminal (ab135055) at 1/500 dilution + HeLa total cell extracts

Anti-BARD1 Antibody (aa108-124) IHC-plus™ - LS-B794 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-BARD1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B794 concentration 10 ug/ml.

Anti-BARD1 Antibody (aa743-760) - LS-C3327 from LifeSpan Bioscience

Prices $265.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-BARD1 Antibody - LS-C48691 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-BARD1 Antibody - LS-C3925 from LifeSpan Bioscience

Prices $465.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-BARD1 Antibody - LS-C35112 from LifeSpan Bioscience

Prices $530.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-BARD1 Antibody - LS-C74643 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-BARD1 Antibody (clone 2059C4a) - LS-C184348 from LifeSpan Bioscience

Prices $335.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2059C4a

Anti-BARD1 Antibody (aa101-131, AP) - LS-C212921 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-BARD1 Antibody (aa101-131, APC) - LS-C212922 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-BARD1 Antibody (aa101-131, FITC) - LS-C212924 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-BARD1 Antibody (aa101-131, Biotin) - LS-C212923 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-BARD1 Antibody (aa101-131, PE) - LS-C212926 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-BARD1 Antibody - LS-C43051 from LifeSpan Bioscience

Prices $355.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Detection of human BARD1 in nuclear extracts of HeLa and U2OS cells and in U2OS cells either expressing endogenous (-) or over-expressing (+) human BARD1 by Western blotting with Rabbit anti-Human BARD1 (LS-C43051). Immunoprecipitation of human Bard1 from nuclear extracts of HeLa cells using AHP871 (2 ug/100ug lysate) or normal rabbit IgG as a control.

Anti-BARD1 Antibody (aa101-131, Azide-free, HRP) - LS-C212928 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-BARD1 Antibody (aa7-221) - LS-C311509 from LifeSpan Bioscience

Prices $250.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-BARD1 Antibody (aa104-135) - LS-C319776 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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