UBE2O

Gene Symbol UBE2O
Entrez Gene 63893
Alt Symbol E2-230K
Species Human
Gene Type protein-coding
Description ubiquitin-conjugating enzyme E2O
Other Description E2/E3 hybrid ubiquitin-protein ligase UBE2O|ubiquitin carrier protein O|ubiquitin-conjugating enzyme E2 of 230 kDa|ubiquitin-conjugating enzyme E2-230K|ubiquitin-protein ligase O
Swissprots Q86UA4 Q9H9B2 Q9BSW1 Q8TBN1 Q9H6E6 Q9C0C9 A6NDU5 Q8N425 Q6PIZ2 Q9H7E4 Q69YP4
Accessions EAW89395 EAW89396 EAW89397 EAW89398 EAW89399 Q9C0C9 AB051521 BAB21825 AK000926 AK022940 BAB14320 AK024657 BAB14948 AK025999 BAB15313 AK225412 AL832432 CAH10644 BC004525 AAH04525 BC007822 BC022237 AAH22237 BC025977 AAH25977 BC036820 AAH36820 BC051868 AAH51868 DA092358 XM_005257567 XP_005257624 XM_011525107 XP_011523409 XM_011525108 XP_011523410 XM_011525109 XP_011523411 NM_022066 NP_071349
Function E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin- associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950). Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASH1, leading to promote endosomal F-actin assembly (PubMed:23452853). {ECO:0000269|PubMed:23381138, ECO:0000269|PubMed:23452853,
Subcellular Location Cytoplasm {ECO:0000269|PubMed:24703950}. Nucleus {ECO:0000269|PubMed:24703950}. Note=Mainly localizes to the cytoplasm.
Tissue Specificity Predominantly expressed in skeletal muscle and heart. {ECO:0000269|PubMed:11311559}.
Top Pathways Ubiquitin mediated proteolysis

UBE2O (N-12) - sc-109125 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
UBE2O (N-12): sc-109125. Western blot analysis of UBE2O expression in HeLa (A) and LADMAC (B) whole cell lysates.

UBE2O (S-17) - sc-109126 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
UBE2O (S-17): sc-109126. Western blot analysis of UBE2O expression in HeLa (A), MDA-MB-231 (B), SK-MEL-24 (C) and HEL 92.1.7 (D) whole cell lysates.

Anti-E2 230K antibody - ab111707 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-E2 230K antibody (ab111707) at 1/1000 dilution + A431 whole cell lysate at 30 µg

Anti-E2 230K antibody - ab118832 from Abcam

Prices $407.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
ab118832, at 3.75 µg/ml, staining E2 230K in formalin fixed, paraffin embedded Human skeletal muscle tissue by Immunohistochemistry.

Anti-E2 230K antibody - ab102544 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-E2 230K antibody (ab102544) at 1 µg/ml + RPMI-8226 cell lysate at 10 µg

Anti-E2 230K antibody - ab13728 from Abcam

Prices $242.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-UBE2O Antibody (aa1079-1128) - LS-C116696 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
UBE2O antibody LS-C116696 Western blot of RPMI-8226 cell lysate.

Anti-UBE2O Antibody (aa1279-1292) IHC-plus™ - LS-B5700 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-UBE2O antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5700 concentration 3.75 ug/ml.

Anti-UBE2O Antibody (aa725-775) - LS-C288651 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human UBE2O by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-UBE2O antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

UBE2O Antibody - NBP2-20787 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: UBE2O Antibody [NBP2-20787] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:1000.

UBE2O Antibody - NBP1-32020 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: UBE2O Antibody [NBP1-32020] - Sample (30 ug of whole cell lysate) A: HeLa 5% SDS PAGE, antibody diluted at 1:500.

UBE2O Antibody - NB100-1149 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Immunohistochemistry-Paraffin: UBE2O Antibody [NB100-1149] - Staining of Human Heart. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

UBE2O Antibody - H00063893-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: UBE2O Antibody [H00063893-D01P] - Analysis of UBE2O expression in transfected 293T cell line by UBE2O polyclonal antibody.Lane 1: UBE2O transfected lysate(81.70 KDa).Lane 2: Non-transfected lysate.

UBE2O Antibody (2C10) - H00063893-M06 from Novus Biologicals

Prices $349.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2C10

UBE2O Antibody - NBP1-03336 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: UBE2O Antibody [NBP1-03336] - Detection of Human UBE2O bon HeLa whole cell lysate using NBP1-03336.

UBE2O Polyclonal Antibody - bs-8380R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-UBE2O antibody produced in rabbit - SAB1401795 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Polyclonal Anti-UBE2O, antibody produced in rabbit, purified immunoglobulin: <B>Cat. No. SAB1401795</B>: Antibody concentration: 0.1 - 5.0 μg/mL. Specific band of ~81.70 kDa using UBE2O transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Monoclonal Anti-UBE2O antibody produced in mouse - SAB1403342 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2C10
<B>Immunoblotting</B><BR/>Monoclonal Anti-UBE2O: <B>Cat. No. SAB1403342</B>: Antibody concentration: 1 μg/mL. Specific band of ~38.2 kDa using immunogen protein lysate.

Anti-UBE2O antibody produced in rabbit - SAB2106136 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-UBE2O: <B>Cat. No. SAB2106136</B>: UBE2O in RPMI-8226 cell lysates with UBE2O antibody at 1.0 μg/mL.

Anti-UBE2O (C-terminal) antibody produced in goat - SAB2501903 from Sigma-Aldrich

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-UBE2O (C-terminal) <B>Cat. No. SAB2501903</B>: Immunohistochemistry (4 μg/mL) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

UBE2O antibody - middle region (ARP57583_P050) - ARP57583_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

E2-230K--UBE2O-Antibody-C-Term - AF2304a from Abgent, a WuXi AppTec company

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
AF2304a (4 µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

E2-230K / UBE2O antibody, C-term - GTX13728 from GeneTex

Prices $289.00
Sizes 100 μg
Host Goat
Clonality Polyclonal
Anti-E2-230K / UBE2O antibody, C-term used in IHC (Formalin-fixed paraffin-embedded sections). GTX13728

Goat Anti-E2-230K / UBE2O Antibody (OAEB00540) - OAEB00540 from Aviva Systems Biology

Prices $289.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

UBE2O antibody - 70R-21111 from Fitzgerald

Prices $375.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
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