Gene Symbol | HS3ST5 |
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Entrez Gene | 222537 |
Alt Symbol | 3-OST-5, 3OST5, HS3OST5, NBLA04021 |
Species | Human |
Gene Type | protein-coding |
Description | heparan sulfate (glucosamine) 3-O-sulfotransferase 5 |
Other Description | h3-OST-5|heparan sulfate 3-O-sulfotransferase 5|heparan sulfate 3-OST-5|heparan sulfate D-glucosaminyl 3-O-sulfotransferase 5|heparan sulfate glucosamine 3-O-sulfotransferase 5 |
Swissprots | Q52LI2 Q8N285 Q8IZT8 A8K1J2 |
Accessions | EAW48251 Q8IZT8 AB074164 BAE45734 AF503292 AAN37737 AK091074 AK126930 AK289907 BAF82596 BC093911 AAH93911 BC093913 AAH93913 XM_006715379 XP_006715442 XM_011535587 XP_011533889 XM_011535588 XP_011533890 XR_942347 NM_153612 NP_705840 |
Function | Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site. Also generates GlcUA- GlcNS or IdoUA-GlcNS and IdoUA2S-GlcNH2. The substrate-specific O- sulfation generates an enzyme-modified heparan sulfate which acts as a binding receptor to Herpes simplex virus-1 (HSV-1) and permits its entry. {ECO:0000269|PubMed:12138164}. |
Subcellular Location | Golgi apparatus membrane {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}. |
Tissue Specificity | Highly expressed in skeletal muscle and fetal brain, and also found in adult brain, spinal cord, cerebellum and colon. {ECO:0000269|PubMed:12138164, ECO:0000269|PubMed:12740361}. |
Top Pathways | Glycosaminoglycan biosynthesis - heparan sulfate / heparin |
HS3ST5 CRISPR/Cas9 KO Plasmid (h) - sc-415157 from Santa Cruz Biotechnology
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HS3ST5 HDR Plasmid (h) - sc-415157-HDR from Santa Cruz Biotechnology
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HS3ST5 Double Nickase Plasmid (h) - sc-415157-NIC from Santa Cruz Biotechnology
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HS3ST5 Double Nickase Plasmid (h2) - sc-415157-NIC-2 from Santa Cruz Biotechnology
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HS3ST5 CRISPR Activation Plasmid (h) - sc-415157-ACT from Santa Cruz Biotechnology
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HS3ST5 CRISPR Activation Plasmid (h2) - sc-415157-ACT-2 from Santa Cruz Biotechnology
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HS3ST5 Lentiviral Activation Particles (h) - sc-415157-LAC from Santa Cruz Biotechnology
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HS3ST5 Lentiviral Activation Particles (h2) - sc-415157-LAC-2 from Santa Cruz Biotechnology
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HS3ST5 siRNA (h) - sc-95068 from Santa Cruz Biotechnology
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HS3ST5 shRNA Plasmid (h) - sc-95068-SH from Santa Cruz Biotechnology
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HS3ST5 shRNA (h) Lentiviral Particles - sc-95068-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU019301 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN10311 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND10311 from Sigma-Aldrich
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HS3ST5 siRNA (Human) - CRJ6567 from Cohesion Biosciences
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HS3ST5 - MBS8206424 from MyBioSource
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Human HS3ST5 siRNA - orb277068 from Biorbyt
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HS3ST5 shRNA Plasmid (Human) - CHJ6567 from Cohesion Biosciences
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HS3ST5 miRNA 3'UTR clone - MiUTR3H-10594 from Creative biogene
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