PLAG1

Gene Symbol	PLAG1
Entrez Gene	5324
Alt Symbol	PSA, SGPA, ZNF912
Species	Human
Gene Type	protein-coding
Description	pleiomorphic adenoma gene 1
Other Description	COL1A2/PLAG1 fusion\|zinc finger protein PLAG1
Swissprots	Q9Y4L2 B4DLC2 Q59GH8 Q6DJT9
Accessions	CAB42491 EAW86774 EAW86775 Q6DJT9 AB209131 BAD92368 AF221549 AF221550 AK296933 BAG59484 AK313663 BAG36415 BC075047 AAH75047 BC075048 AAH75048 DA225321 DC347559 EF525168 EF525169 EF525170 ABW87255 EU446900 ABZ92429 U65002 AAC50995 U78783 XM_005251260 XP_005251317 XM_011517544 XP_011515846 NM_001114634 NP_001108106 NM_001114635 NP_001108107 NM_002655 NP_002646
Function	Transcription factor whose activation results in up- regulation of target genes, such as IGFII, leading to uncontrolled cell proliferation: when overexpressed in cultured cells, higher proliferation rate and transformation are observed. Other target genes such as CRLF1, CRABP2, CRIP2, PIGF are strongly induced in cells with PLAG1 induction. Proto-oncogene whose ectopic expression can trigger the development of pleomorphic adenomas of the salivary gland and lipoblastomas. Overexpression is associated with up-regulation of IGFII, is frequently observed in hepatoblastoma, common primary liver tumor in childhood. Cooperates with CBFB-MYH11, a fusion gene important for myeloid leukemia. {ECO:0000269\|PubMed:11888928, ECO:0000269\|PubMed:14695992, ECO:0000269\|PubMed:14712223}.
Subcellular Location	Nucleus {ECO:0000269\|PubMed:10646861, ECO:0000269\|PubMed:11882654, ECO:0000269\|PubMed:16207715}. Note=Strong nucleolar localization when sumoylation is inhibited.
Tissue Specificity	Expressed in fetal tissues such as lung, liver and kidney. Not detected or weak detection in normal adult tissues, but highly expressed in salivary gland with benign or malignant pleiomorphic adenomas with or without 8q12 aberrations, with preferential occurrence in benign tumors. {ECO:0000269\|PubMed:10029085, ECO:0000269\|PubMed:14695992, ECO:0000269\|PubMed:9020842, ECO:0000269\|PubMed:9722527}.

PLAG1 (S-15) - sc-20320 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-PLAG1 antibody - ab154579 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

ab154579 staining PLAG1 in the HEK293T cell line from Human embryonic kidney by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.3% and blocked with 10% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/400 in PBS + 1% BSA + 0.3% Triton) for 16 hours at 4°C. A Rhodamine-conjugated Sheep anti-rabbit polyclonal(1/10) was used as the secondary antibody.See Abreview

Anti-PLAG1 antibody - ab80267 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-PLAG1 antibody - ab87662 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PLAG1 antibody (ab87662) at 1 µg/ml (in 5% skim milk/ PBS buffer ) + OVCAR3 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-PLAG1 antibody - ab55652 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

Western blot against tagged recombinant protein immunogen using ab55652 PLAG1 antibody at 1ug/ml.

Anti-PLAG1 antibody - C-terminal - ab187387 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-PLAG1 antibody - C-terminal (ab187387) at 1/1000 dilutionLane 1 : Mouse brain total cell extractLane 2 : Mouse thymus total cell extractLane 3 : HeLa total cell extract

Anti-PLAG1 Antibody (aa8-35, AP) - LS-C216814 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PLAG1 Antibody (aa8-35, APC) - LS-C216815 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PLAG1 Antibody (aa8-35, Biotin) - LS-C216817 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PLAG1 Antibody (aa8-35, Azide-free, HRP) - LS-C216819 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PLAG1 Antibody (aa8-35, PE) - LS-C216820 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PLAG1 Antibody (aa8-35, FITC) - LS-C216818 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PLAG1 Antibody (aa15-44) - LS-C324088 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PLAG1 Antibody (clone 1F11) - LS-C139243 from LifeSpan Bioscience

Prices	$355.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1F11

Detection limit for recombinant GST tagged PLAG1 is approximately 1 ng/ml as a capture antibody.

Anti-PLAG1 Antibody (N-Terminus) - LS-C80185 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Hela cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-PLAG1 Antibody (C-Terminus) - LS-C110247 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

PLAG1 antibody LS-C110247 Western blot of OVCAR-3 cell lysate.

Anti-PLAG1 Antibody (aa8-35) - LS-C163767 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

PLAG1 Antibody western blot of NCI-H460,K562 cell line lysates (35 ug/lane). The PLAG1 antibody detected the PLAG1 protein (arrow).

PLAG1 Antibody (3B7) - H00005324-M02 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	3B7

Western Blot: PLAG1 Antibody (3B7) [H00005324-M02] - Western Blot detection against Immunogen (36.52 KDa).

PLAG1 Antibody - NBP2-15076 from Novus Biologicals

Prices	$329.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

PLAG1 Antibody - NBP2-15075 from Novus Biologicals

Prices	$329.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: PLAG1 Antibody [NBP2-15075] - Sample (30 ug of whole cell lysate) A: PC-3 7.5% SDS PAGE, antibody diluted at 1:3000.

PLAG1 Antibody - NBP2-15074 from Novus Biologicals

Prices	$329.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: PLAG1 Antibody [NBP2-15074] - Sample (30 ug of whole cell lysate) A: U87-MG 7.5% SDS PAGE; antibody diluted at 1:1000.

PLAG1 Antibody (1F11) - H00005324-M07 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1F11

PLAG1 Antibody - H00005324-A01 from Novus Biologicals

Prices	$269.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

PLAG1 Polyclonal Antibody - bs-5969R from Bioss

Prices	$269.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Monoclonal Anti-PLAG1 antibody produced in mouse - SAB1404215 from Sigma-Aldrich

Prices	$347.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1F11

<B>Immunoblotting</B><BR/>Monoclonal Anti-PLAG1: <B>Cat. No. SAB1404215</B>: Antibody concentration: 1 μg/mL. Specific band of ~36.9 kDa using immunogen protein lysate.

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