PNPT1
| Gene Symbol | PNPT1 |
|---|---|
| Entrez Gene | 87178 |
| Alt Symbol | COXPD13, DFNB70, OLD35, PNPASE, old-35 |
| Species | Human |
| Gene Type | protein-coding |
| Description | polyribonucleotide nucleotidyltransferase 1 |
| Other Description | 3'-5' RNA exonuclease OLD35|PNPase 1|PNPase old-35|polynucleotide phosphorylase 1|polynucleotide phosphorylase-like protein|polyribonucleotide nucleotidyltransferase 1, mitochondrial |
| Swissprots | Q9BVX0 Q7Z7D1 Q53SU0 Q68CN1 Q9BRU3 Q8IWX1 Q96T05 Q8TCS8 |
| Accessions | AAY24271 CAR80228 CBX83686 EAX00086 EAX00087 Q8TCS8 AJ458465 CAD30289 AK307589 AW731791 AY027528 AAK13047 AY290863 AAP44472 BC000862 AAH00862 BC005986 AAH05986 BC009057 BC021170 BC053660 AAH53660 BU943827 CR749867 CAH18709 DA998523 DB461021 XM_005264629 XP_005264686 XM_011533142 XP_011531444 NM_033109 NP_149100 |
| Function | RNA-binding protein implicated in numerous RNA metabolic processes. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction. Mitochondrial intermembrane factor with RNA-processing exoribonulease activity. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double- stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Required for correct processing and polyadenylation of mitochondrial mRNAs. Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, into the mitochondrial matrix. Plays a role in mitochondrial morphogenesis and respiration; regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels. In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity; degrades c-m |
| Subcellular Location | Cytoplasm. Mitochondrion. Mitochondrion intermembrane space; Peripheral membrane protein. |
| Top Pathways | RNA degradation, Purine metabolism, Pyrimidine metabolism |
PNPase CRISPR/Cas9 KO Plasmid (h) - sc-404406 from Santa Cruz Biotechnology
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PNPase HDR Plasmid (h) - sc-404406-HDR from Santa Cruz Biotechnology
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PNPase Double Nickase Plasmid (h) - sc-404406-NIC from Santa Cruz Biotechnology
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PNPase Double Nickase Plasmid (h2) - sc-404406-NIC-2 from Santa Cruz Biotechnology
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PNPase CRISPR Activation Plasmid (h) - sc-404406-ACT from Santa Cruz Biotechnology
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PNPase CRISPR Activation Plasmid (h2) - sc-404406-ACT-2 from Santa Cruz Biotechnology
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PNPase Lentiviral Activation Particles (h) - sc-404406-LAC from Santa Cruz Biotechnology
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PNPase Lentiviral Activation Particles (h2) - sc-404406-LAC-2 from Santa Cruz Biotechnology
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PNPase siRNA (h) - sc-61371 from Santa Cruz Biotechnology
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PNPase shRNA Plasmid (h) - sc-61371-SH from Santa Cruz Biotechnology
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PNPase shRNA (h) Lentiviral Particles - sc-61371-V from Santa Cruz Biotechnology
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PNPT1 RNAi - H00087178-R01 from Novus Biologicals
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MISSION® esiRNA - EHU050911 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN17071 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND17071 from Sigma-Aldrich
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PNPT1 siRNA (Human) - CRJ3844 from Cohesion Biosciences
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shRNA set against Human PNPT1 (NM_033109.3) - SHH378876 from Creative biogene
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Human PNPT1 siRNA - orb274345 from Biorbyt
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PNPT1 shRNA Plasmid (Human) - CHJ3844 from Cohesion Biosciences
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