SMC4

Gene Symbol SMC4
Entrez Gene 10051
Alt Symbol CAP-C, CAPC, SMC-4, SMC4L1, hCAP-C
Species Human
Gene Type protein-coding
Description structural maintenance of chromosomes 4
Other Description SMC protein 4|SMC4 structural maintenance of chromosomes 4-like 1|XCAP-C homolog|chromosome-associated polypeptide C|structural maintenance of chromosomes protein 4
Swissprots Q8NDL4 O95752 A6NLT9 Q9NTJ3 Q9UNT9 D3DNL8
Accessions EAW78639 EAW78640 EAW78641 EAW78642 Q9NTJ3 AB019987 BAA73535 AF092564 AAC72361 AK002200 AK026361 AK122939 BAG53807 AK128143 BAG54637 AK225437 AK307508 AK315070 AL136877 CAB66811 AL833949 CAD38803 BC001557 AAH01557 BC065259 AAH65259 BC070161 AAH70161 BC106033 AAI06034 BC146370 AAI46371 BC148796 AAI48797 BI090399 BQ575962 DB444342 XM_006713459 XP_006713522 XM_011512311 XP_011510613 XM_011512312 XP_011510614 XM_011512313 XP_011510615 NM_001002799 NM_001002800 NP_001002800 NM_001288753 NP_001275682 NM_005496 NP_005487
Function Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. {ECO:0000269|PubMed:11136719}.
Subcellular Location Nucleus {ECO:0000269|PubMed:10958694}. Cytoplasm {ECO:0000269|PubMed:10958694}. Chromosome {ECO:0000269|PubMed:10958694}. Note=In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
Tissue Specificity Widely expressed. Higher expression in testis, colon, thymus. {ECO:0000269|PubMed:10319587}.

SMC4 (D14E2) Rabbit mAb - 5547 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D14E2
Western blot analysis of extracts from various cell lines using SMC4 (D14E2) Rabbit mAb.

SMC4 (C-17) - sc-10715 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SMC4 (C-17): sc-10715. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

SMC4 (T-18) - sc-10713 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SMC4 (T-18): sc-10713. Western blot analysis of SMC4 expression in HeLa (A), K-562 (B) and Jurkat (C) nuclear extracts and HeLa whole cell lysate (D).

Anti-SMC4 antibody - ab17957 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling SMC4 with ab17957 at 5µg/ml. Cells were fixed with methanol/actetone and blocked with 3% BSA in PBS. A Rhodamine-conjugated anti-rabbit IgG was used as the secondary antibody. Cells were counterstained with DAPI.

Anti-SMC4 antibody - ab67753 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMC4 antibody - ab194487 from Abcam

Prices $376.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Anti-SMC4 antibody (ab194487) at 1/500 dilution + Daoy cell lysate at 50 µg

Anti-SMC4 antibody [EPR13365] - ab179803 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13365
All lanes : Anti-SMC4 antibody [EPR13365] (ab179803) at 1/1000 dilutionLane 1 : Ramos cell lysateLane 2 : 293T cell lysateLane 3 : HeLa cell lysateLane 4 : Raji cell lysate

Anti-SMC4 antibody [EPR13366] - C-terminal - ab179831 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13366
All lanes : Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831) at 1/1000 dilutionLane 1 : Ramos cell lysateLane 2 : 293T cell lysateLane 3 : HeLa cell lysateLane 4 : Raji cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-SMC4 Antibody (aa493-504) - LS-C12387 from LifeSpan Bioscience

Prices $445.00
Sizes 200 µg
Host Rabbit
Clonality Polyclonal

Anti-SMC4 Antibody - LS-C91825 from LifeSpan Bioscience

Prices $575.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SMC4 Antibody (C-Terminus) - LS-C91826 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SMC4 Antibody (N-Terminus) - LS-C144798 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SMC4 Antibody - LS-C147002 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SMC4 Antibody (aa1109-1127) - LS-C147962 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-SMC4 Antibody (aa36-85) - LS-C111084 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
SMC4 antibody LS-C111084 Western blot of Jurkat lysate.

Anti-SMC4 Antibody (aa1080-1129) - LS-C111085 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
SMC4 antibody LS-C111085 Western blot of MCF7 cell lysate.

Anti-SMC4 Antibody (C-Terminus) - LS-C287005 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human SMC4 by Western Blot and Immuno-precipitation. Samples: Whole cell lysate (90 ug for WB; 0.5 mg for IP/WB) from 293T cells (lanes 1, 3 and 5), normal human lymphoblasts (lane 2) or SMC4 transfected 239T cells (lanes 4, 6 and 7) separated on an 8% SDS-PAGE gel. Antibodies: Affinity purified rabbit anti-SMC4 used at 0.2 or 0.1 ug/ml for WB and 5 ug for IP. Detection: Chemiluminescence with the indicated exposure times.

Anti-SMC4 Antibody (aa600-650) - LS-C287004 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Localization of SMC4 to Mitotic Chromosomes by Immunofluorescence. Samples: HeLa cells grown on coverslips, fixed with methanol/acetone and blocked with 3% BSA in PBS. Antibody: Affinity purified rabbit anti-SMC4 antibody used at 5 ug/ml. Detection: Rhodamine labeled anti-rabbit secondary antibody. Cells were counterstained with DAPI.

Anti-SMC4 Antibody (aa281-297) IHC-plus™ - LS-B2066 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-SMC4 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2066 concentration 5 ug/ml.

Anti-SMC4 Antibody (aa47-61) IHC-plus™ - LS-B2067 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-SMC4 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2067 concentration 5 ug/ml.

SMC4 Antibody - NB100-375 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: SMC4 Antibody [NB100-375] - Whole cell lysate (90 mcg for WB; 0.5 mg for IP/WB) from 293T cells (lanes 1, 3 and 5), normal human lymphoblasts (lane 2) or SMC4 transfected 239T cells (lanes 4, 6 and 7) separated on an 8% SDS-PAGE gel. Affinity purified rabbit anti-SMC4 used at 0.2 or 0.1 mcg/ml for WB and 5 mcg for IP.

SMC4 Antibody - NBP1-86635 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: SMC4 Antibody [NBP1-86635] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

SMC4 Antibody - NB100-374 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: SMC4 Antibody [NB100-374] - HeLa cells grown on coverslips, fixed with methanol/acetone and blocked with 3% BSA in PBS. Antibody used at 5 ug/ml. Detection: Rhodamine labeled anti-rabbit secondary antibody. Cells were counterstained with DAPI.

SMC4 Antibody - H00010051-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: SMC4 Antibody [H00010051-A01] - SMC4L1 polyclonal antibody (A01), Lot # 050919JC01. Western Blot analysis of SMC4L1 expression in Daoy.

SMC4 Antibody - H00010051-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
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