Gene Symbol | RNF34 |
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Entrez Gene | 80196 |
Alt Symbol | CARP-1, CARP1, RFI, RIF, RIFF, hRFI |
Species | Human |
Gene Type | protein-coding |
Description | ring finger protein 34, E3 ubiquitin protein ligase |
Other Description | E3 ubiquitin-protein ligase RNF34|FYVE-RING finger protein MOMO|RING finger protein RIFF|caspase regulator CARP1|caspases-8 and -10-associated RING finger protein 1|human RING finger homologous to inhibitor of apoptosis protein |
Swissprots | B7Z933 Q969K3 Q8NG47 Q9H6W8 |
Accessions | EAW98262 EAW98263 EAW98264 Q969K3 AB084914 BAC11802 AF306709 AAK51328 AK024957 AK025439 BAB15132 AK096994 AK130355 AK225285 AK304366 BAH14169 AL557811 AY098934 AAM29180 BC007826 AAH07826 BC039029 BC046174 BI559962 BT007283 AAP35947 CR457342 CAG33623 DA887995 DB242808 NM_001256858 NP_001243787 NM_025126 NP_079402 NM_194271 NP_919247 |
Function | E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis (PubMed:15069192). May mediate 'Lys-48'-linked polyubiquitination of RIPK1 and its subsequent proteasomal degradation thereby indirectly regulating the tumor necrosis factor-mediated signaling pathway (Ref.13). Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation (PubMed:17121812). Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN (PubMed:18382127). Mediates PPARGC1A proteasomal degradation probably through ubiquitination thereby indirectly regulating the metabolism of brown fat cells (PubMed:22064484). Possibly involved in innate immunity, throu |
Subcellular Location | Cell membrane {ECO:0000269|PubMed:15069192}; Peripheral membrane protein {ECO:0000305}. Endomembrane system {ECO:0000250|UniProtKB:Q6AYH3}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q6AYH3}. Nucleus {ECO:0000269|PubMed:22064484}. Nucleus speckle {ECO:0000269|PubMed:12118383}. Cytoplasm, cytosol {ECO:0000269|PubMed:15069192}. |
Tissue Specificity | Ubiquitous. Detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes. {ECO:0000269|PubMed:12118383, ECO:0000269|PubMed:15069192}. |
hRIF CRISPR/Cas9 KO Plasmid (h) - sc-413358 from Santa Cruz Biotechnology
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hRIF HDR Plasmid (h) - sc-413358-HDR from Santa Cruz Biotechnology
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hRIF Double Nickase Plasmid (h) - sc-413358-NIC from Santa Cruz Biotechnology
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hRIF Double Nickase Plasmid (h2) - sc-413358-NIC-2 from Santa Cruz Biotechnology
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hRIF CRISPR Activation Plasmid (h) - sc-413358-ACT from Santa Cruz Biotechnology
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hRIF CRISPR Activation Plasmid (h2) - sc-413358-ACT-2 from Santa Cruz Biotechnology
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hRIF Lentiviral Activation Particles (h) - sc-413358-LAC from Santa Cruz Biotechnology
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hRIF Lentiviral Activation Particles (h2) - sc-413358-LAC-2 from Santa Cruz Biotechnology
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hRIF siRNA (h) - sc-75301 from Santa Cruz Biotechnology
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hRIF shRNA Plasmid (h) - sc-75301-SH from Santa Cruz Biotechnology
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hRIF shRNA (h) Lentiviral Particles - sc-75301-V from Santa Cruz Biotechnology
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RNF34 RNAi - H00080196-R01 from Novus Biologicals
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RNF34 RNAi - H00080196-R02 from Novus Biologicals
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MISSION® esiRNA - EHU074201 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN18432 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND18432 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR12323 from Sigma-Aldrich
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RNF34 siRNA (Human) - CRJ2794 from Cohesion Biosciences
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RNF34 - MBS8207168 from MyBioSource
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shRNA set against Human RNF34 (NM_194271.2) - SHH397300 from Creative biogene
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RNF34 shRNA Plasmid (Human) - CHJ2794 from Cohesion Biosciences
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RNF34 miRNA 3'UTR clone - MiUTR3H-09898 from Creative biogene
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RNF34 miRNA 3'UTR clone - MiUTR3H-09899 from Creative biogene
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