ALAS1

Gene Symbol ALAS1
Entrez Gene 211
Alt Symbol ALAS, ALAS-H, ALAS3, ALASH, MIG4
Species Human
Gene Type protein-coding
Description 5'-aminolevulinate synthase 1
Other Description 5-aminolevulinate synthase, nonspecific, mitochondrial|5-aminolevulinic acid synthase 1|aminolevulinate, delta-, synthase 1|delta-ALA synthase 1|delta-aminolevulinate synthase 1|migration-inducing protein 4
Swissprots P13196
Accessions CAD33292 EAW65188 EAW65189 EAW65190 P13196 AB063322 BAB93514 AI868691 AK293190 BAG56730 AK300993 BAG62612 AK312566 BAG35461 AU279391 AY260745 AAP94018 BC011798 AAH11798 BM546974 BU173391 CD014147 DA691060 JF432123 ADZ15340 X56351 CAA39794 Y00451 CAA68506 XM_011533477 XP_011531779 XM_011533478 XP_011531780 NM_000688 NP_000679 NM_001304443 NP_001291372 NM_001304444 NP_001291373 NM_199166 NP_954635
Subcellular Location Mitochondrion matrix.
Top Pathways Glycine, serine and threonine metabolism, Porphyrin and chlorophyll metabolism

ALAS-H (A-6) - sc-365153 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ALAS-H (A-6): sc-365153. Western blot analysis of ALAS-H expression in HEL 92.1.7 whole cell lysate.

ALAS-H (C-6) - sc-137094 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ALAS-H (C-6): sc-137094. Western blot analysis of ALAS-H expression in non-transfected: sc-117752 (A) and mouse ALAS-H transfected: sc-118326 (B) 293T whole cell lysates.

ALAS-H (F-5) - sc-137093 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ALAS-H (F-5): sc-137093. Western blot analysis of ALAS-H expression in non-transfected: sc-117752 (A) and mouse ALAS-H transfected: sc-118326 (B) 293T whole cell lysates.

ALAS-H (H-154) - sc-50531 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
ALAS-H (H-154): sc-50531. Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of cortical cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

ALAS-H (M-154) - sc-50532 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

ALAS-H (N-20) - sc-32333 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Alas1 antibody - ab54758 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Overlay histogram showing HepG2 cells stained with ab54758 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54758, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Alas1 antibody - ab115216 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Human skin (formalin fixed, paraffin embedded) stained with ab115216 at 5 ug/ml followed by biotinylated Goat anti Rabbit IgG secondary antibody, alkaline phosphatase streptavidin and chromogen.

Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker - ab154860 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10247
Immunofluorescent analysis of HepG2 cells labeling Alas1 with ab154860 at 1/250 dilution.

Anti-Alas1 antibody - Mitochondrial Marker - ab101681 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab101681 stained T47D cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101681, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab101681, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-ALAS1 Antibody - LS-C185224 from LifeSpan Bioscience

Prices $800.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ALAS1 Antibody (aa57-308, clone 1C5) - LS-C340448 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1C5
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALAS1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALAS1 (1:2000).

Anti-ALAS1 Antibody (aa1-250) - LS-C334773 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using ALAS1 antibody.

Anti-ALAS1 Antibody (aa146-195) - LS-C81849 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Fetal lung lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-ALAS1 Antibody (aa46-95) - LS-C206108 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ALAS1 antibody Western Blot of 293T. Antibody dilution: 1 ug/ml.

Anti-ALAS1 Antibody (clone 3G10) - LS-C196635 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3G10
ALAS1 monoclonal antibody (M01), clone 3G10 Western Blot analysis of ALAS1 expression in JAR.

Anti-ALAS1 Antibody (aa480-530) IHC-plus™ - LS-B2013 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-ALAS1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2013 concentration 5 ug/ml.

Anti-ALAS1 Antibody (aa59-69) - LS-C148867 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-ALAS1 Antibody (aa131-144) - LS-C148870 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Alas1 Antibody - NBP1-91656 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Alas1 Antibody - NBP1-54668 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Alas1 Antibody (3G10) - H00000211-M01 from Novus Biologicals

Prices $349.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3G10
Western Blot: Alas1 Antibody (3G10) [H00000211-M01] - ALAS1 monoclonal antibody (M01), clone 3G10 Western Blot analysis of ALAS1 expression in JAR ( Cat # L003V1 ).

Alas1 Antibody - NBP2-15333 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Alas1 Antibody [NBP2-15333] - Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE; antibody diluted at 1:1000.

Alas1 Antibody (2D5) - H00000211-M03 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2D5

ALAS1 Polyclonal Antibody - bs-9527R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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