CENPT

Gene Symbol CENPT
Entrez Gene 80152
Alt Symbol C16orf56, CENP-T
Species Human
Gene Type protein-coding
Description centromere protein T
Other Description interphase centromere complex protein 22
Swissprots Q96NK9 Q96I29 Q96IC6 Q9H901 Q96BT3
Accessions EAW83178 EAW83179 Q96BT3 AK023173 BAB14445 AK055237 BAB70884 AK056097 BAG51624 AK125366 AK225399 AK291527 BAF84216 AK297570 BAG59961 AK300887 BAG62529 AK301621 BAG63106 BC007642 AAH07642 BC007864 AAH07864 BC015202 AAH15202 BC042204 BX648617 CD242240 XM_006721277 XP_006721340 XM_011523344 XP_011521646 XM_011523345 XP_011521647 NM_025082 NP_079358
Function Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Part of a nucleosome- associated complex that binds specifically to histone H3- containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPT has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis. {ECO:0000269|PubMed:16716197, ECO:0000269|PubMed:21529714, ECO:0000269|PubMed:21695110}.
Subcellular Location Nucleus. Chromosome, centromere, kinetochore. Note=Constitutively localizes to centromeres throughout the cell cycle, and to kinetochores during mitosis. Localizes to the inner kinetochore, and may connect it to the outer kinetochore via its N-terminus.

CENP-T Antibody - 12494 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using CENP-T Antibody.

CENP-T (G-14) - sc-160227 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

CENP-T (K-13) - sc-160228 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-CENPT antibody - ab109470 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-CENPT antibody (ab109470) at 1 µg/mlLane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/mlLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-CENPT antibody - ab86595 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-CENPT antibody (ab86595) at 0.1 µg/mlLane 1 : Whole cell lysate from HeLa cells at 50 µgLane 2 : Whole cell lysate from HeLa cells at 15 µgLane 3 : Whole cell lysate from HeLa cells at 5 µgLane 4 : Whole cell lysate from 293T cells at 50 µg

Anti-CENPT antibody - ab114120 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Detection of CENPT by Western Blot of Immunprecipitate. Lane 1: An antibody to CENPT at 1µg/ml staining CENPT in HeLa whole cell lysate immunoprecipitated using ab114120 at 10µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: Control IgG Detection: Chemiluminescence with exposure time of 3 seconds.

Anti-CENPT antibody - ab169034 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-CENPT antibody (ab169034) at 1 µg/mlLane 1 : CENPT-transfected 293T cell line lysateLane 2 : Non-transfected 293T cell line lysateSecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilutiondeveloped using the ECL technique

Anti-CENPT Antibody (clone 42F10) - LS-C179532 from LifeSpan Bioscience

Prices $380.00
Sizes 100 µg
Host Rat
Clonality Monoclonal
Clone 42F10

Anti-CENPT Antibody (aa520-561) - LS-C286226 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CENPT Antibody (aa480-520) - LS-C289064 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CENPT Antibody (aa438-487) - LS-C186937 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of Human HepG2. CENPT antibody dilution 1.0 ug/ml.

CENPT Antibody - NBP2-34093 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

CENPT Antibody - NBP1-28744 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: CENPT Antibody [NBP1-28744] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CENP-T antibody NBP1-28744 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. CENP-T was also immunoprecipitated by rabbit anti-CENP-T antibody NBP1-28698, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).

CENPT Antibody - NBP1-28698 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunoprecipitation: CENPT Antibody [NBP1-28698] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CENP-T antibody NBP1-28698 used for IP at 10 ug/mg lysate. CENP-T was also immunoprecipitated by rabbit anti-CENP-T antibody NBP1-28744, which recognizes an upstream epitope. For blotting immunoprecipitated CENP-T, NBP1-28744 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

CENPT Antibody - H00080152-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: CENPT Antibody [H00080152-B01] - analysis of C16orf56 expression in transfected 293T cell line by C16orf56 MaxPab polyclonal antibody (B01). Lane 1: C16orf56 transfected lysate (32.20 KDa). Lane 2: Non-transfected lysate.

CENPT Antibody - H00080152-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: CENPT Antibody [H00080152-B01P] - Analysis of CENPT expression in human colon.

CENPT Polyclonal Antibody - bs-6901R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CENPT antibody produced in rabbit - HPA058036 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-CENPT: <B>Cat. No. HPA058036</B>: Immunohistochemical staining of Human cerebral cortex shows moderate nucleolar positivity in neuronal cells.

Anti-CENPT (21-35) antibody produced in rabbit - SAB1104097 from Sigma-Aldrich

Prices $298.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human CENPT was separated on SDS-PAGE, blotted with Anti-CENPT (21-35) (<B>Cat. No. SAB1104097</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:1,000 <BR/>2. Antibody dilution 1:1,000 + CENPT immunizing peptide (human, 21-35)

Anti-CENPT antibody produced in mouse - SAB1408011 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-CENPT: <B>Cat. No. SAB1408011</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~32.9 kDa using CENPT transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-CENPT (261-275) antibody produced in rabbit - SAB1104098 from Sigma-Aldrich

Prices $298.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human CENPT was separated on SDS-PAGE, blotted with Anti-CENPT (261-275) (<B>Cat. No. SAB1104098</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:1,000 <BR/>2. Antibody dilution 1:1,000 + CENPT immunizing peptide (human, 261-275)

CENPT Antibody - C-terminal region (ARP70015_P050) - ARP70015_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

CENPT antibody - GTX51767 from GeneTex

Prices $319.00
Sizes 100 μl
Host Rabbit
Clonality Polyclonal

Anti -CENP-T (Centromere Protein T) - 137424 from USBiological Life Science

Host Rabbit
Clonality Polyclonal

CENP-T antibody - H00080152-B01 from Acris Antibodies

Prices $360.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot analysis of CENPT expression in transfected 293T cell line (H00080152-T01) by CENPT MaxPab polyclonal antibody.Lane 1: C16orf56
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