POLR2E
| Gene Symbol | POLR2E |
|---|---|
| Entrez Gene | 5434 |
| Alt Symbol | RPABC1, RPB5, XAP4, hRPB25, hsRPB5 |
| Species | Human |
| Gene Type | protein-coding |
| Description | polymerase (RNA) II (DNA directed) polypeptide E, 25kDa |
| Other Description | DNA directed RNA polymerase II 23 kda polypeptide|DNA-directed RNA polymerase II 23 kDa polypeptide|DNA-directed RNA polymerase II subunit E|DNA-directed RNA polymerase subunit RPABC1|DNA-directed RNA polymerases I, II, and III subunit RPABC1|RNA polymerases I, II, and III subunit ABC1|RPB5 homolog |
| Swissprots | P19388 O43380 D6W5Y1 Q9BT06 Q6PIH5 B2R6L4 |
| Accessions | AAC03238 ADP92094 ADP92095 ADP92096 ADP92097 ADP92098 ADP92099 ADP92100 ADP92101 ADP92102 ADP92103 ADP92104 ADP92105 ADP92106 ADP92107 ADP92108 ADP92109 ADP92110 ADP92111 ADP92112 ADP92113 ADP92114 ADP92115 ADP92116 ADP92117 ADP92118 ADP92119 ADP92120 ADP92121 ADP92122 ADP92123 ADP92124 ADP92125 ADP92126 ADP92127 ADP92128 ADP92129 ADP92130 ADP92131 ADP92132 ADP92133 EAW69547 EAW69548 EAW69549 EAW69550 P19388 AK122813 AK295975 BAG58751 AK312625 BAG35511 BC004441 AAH04441 BC034144 AAH34144 D38251 BAA07406 DC414435 HQ448367 ADQ32847 J04965 AAA62401 S42643 AAB19339 XM_005259579 XP_005259636 XM_011528070 XP_011526372 NM_002695 NP_002686 |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process (By similarity). {ECO:0000250}. |
| Subcellular Location | Nucleus {ECO:0000269|PubMed:9852112}. |
| Top Pathways | Huntington's disease, Cytosolic DNA-sensing pathway, Epstein-Barr virus infection, Purine metabolism, RNA polymerase |
POLR2E CRISPR/Cas9 KO Plasmid (h) - sc-410947 from Santa Cruz Biotechnology
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POLR2E HDR Plasmid (h) - sc-410947-HDR from Santa Cruz Biotechnology
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POLR2E Double Nickase Plasmid (h) - sc-410947-NIC from Santa Cruz Biotechnology
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POLR2E Double Nickase Plasmid (h2) - sc-410947-NIC-2 from Santa Cruz Biotechnology
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POLR2E CRISPR Activation Plasmid (h) - sc-410947-ACT from Santa Cruz Biotechnology
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POLR2E CRISPR Activation Plasmid (h2) - sc-410947-ACT-2 from Santa Cruz Biotechnology
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POLR2E Lentiviral Activation Particles (h) - sc-410947-LAC from Santa Cruz Biotechnology
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POLR2E Lentiviral Activation Particles (h2) - sc-410947-LAC-2 from Santa Cruz Biotechnology
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POLR2E siRNA (h) - sc-97922 from Santa Cruz Biotechnology
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POLR2E shRNA Plasmid (h) - sc-97922-SH from Santa Cruz Biotechnology
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POLR2E shRNA (h) Lentiviral Particles - sc-97922-V from Santa Cruz Biotechnology
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POLR2E RNAi - H00005434-R01 from Novus Biologicals
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MISSION® esiRNA - EHU137381 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN17115 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND17115 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR04245 from Sigma-Aldrich
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POLR2E siRNA (Human) - CRH3637 from Cohesion Biosciences
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shRNA set against Human POLR2E (NM_002695.3) - SHH379424 from Creative biogene
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shRNA set against Human POLR2E(NM_002695.3) - SHR127460 from Creative biogene
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Human POLR2E siRNA - orb264139 from Biorbyt
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POLR2E shRNA Plasmid (Human) - CHH3637 from Cohesion Biosciences
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POLR2E miRNA 3'UTR clone - MiUTR1H-08075 from Creative biogene
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