DDX5

Gene Symbol DDX5
Entrez Gene 1655
Alt Symbol G17P1, HLR1, HUMP68, p68
Species Human
Gene Type protein-coding
Description DEAD (Asp-Glu-Ala-Asp) box helicase 5
Other Description ATP-dependent RNA helicase DDX5|DEAD (Asp-Glu-Ala-Asp) box polypeptide 5|DEAD box protein 5|DEAD box-5|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD)|RNA helicase p68|probable ATP-dependent RNA helicase DDX5
Swissprots D3DU32 Q53Y61 O75681 B4DLW8 E7ETL9 B5BU21 P17844
Accessions AAB84094 CAA09408 EAW94202 EAW94203 P17844 AB209919 BAD93156 AB451257 BAG70071 AB451382 BAG70196 AK055995 AK297192 BAG59680 AK297753 BAG60103 AK297905 BAG60224 AK310318 AL133585 BC016027 AAH16027 BT006943 AAP35589 BX571764 CAE11890 DA191631 DQ890687 ABM81613 DQ893774 ABM84700 X15729 CAA33751 X52104 CAA36324 XM_005257111 XP_005257168 XM_006721738 XP_006721801 XM_011524456 XP_011522758 XM_011524457 XP_011522759 NM_004396 NP_004387
Function Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for estrogen receptor ESR1 and androgen receptor AR. Increases ESR1 AF-1 domain-mediated transactivation and ESR1 AF-1 and AF-2 domains transcriptional synergistic activity. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcrip
Subcellular Location Nucleus, nucleolus {ECO:0000269|PubMed:10837141, ECO:0000269|PubMed:1996094}.
Top Pathways Spliceosome, Proteoglycans in cancer, Transcriptional misregulation in cancer

DDX5 (D15E10) XP ® Rabbit mAb - 9877 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D15E10
Western blot analysis of extracts from various cell lines using DDX5 (D15E10) XP ® Rabbit mAb.

DDX5 Antibody - 4387 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using DDX5 Antibody.

p68 RNA Helicase (D-7) - sc-365164 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p68 RNA Helicase (D-7): sc-365164. Western blot analysis of p68 RNA Helicase expression in Jurkat whole cell lysate (A) and Jurkat nuclear extract (B).

p68 RNA Helicase (A-5) - sc-166167 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p68 RNA Helicase (A-5): sc-166167. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

p68 RNA Helicase (N-14) - sc-26043 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p68 RNA Helicase (N-14): sc-26043. Western blot analysis of p68 RNA Helicase expression in Jurkat nuclear extract.

p68 RNA Helicase (H-144) - sc-32858 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
p68 RNA Helicase (H-144): sc-32858. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

p68 RNA Helicase (C-20) - sc-26045 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p68 RNA Helicase (C-20): sc-26045. Western blot analysis of p68 RNA Helicase expression in Jurkat (A), A-431 (B) and NIH/3T3 (C) nuclear extracts and mouse embryo tissue extract (D).

Anti-DDX5 (phospho Y593) antibody - ab62255 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab62255 at 1/500 - 1/1000 dilution staining DDX5 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.

Anti-DDX5 antibody - ab21696 from Abcam

Prices $401.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DDX5 antibody (ab21696) at 1 µg/mlLane 1 : HeLa nuclear lysateLane 2 : HeLa nuclear lysate with Human DDX5 peptide (ab22412) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryAlexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution

Anti-DDX5 antibody - ab10261 from Abcam

Prices $391.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
HT1080 cells were fixed with paraformaldehyde permeabilized with Triton (1%) and stained for 1 hr in a 1/100 dilution of the DDX5 antibody and thereafter for 1 hr with a rhodamine conjugated anti-goat secondary. This image was kindly supplied as part of the review submitted by Reinhard Kodym.

Anti-DDX5 antibody - ab109764 from Abcam

Prices $384.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-DDX5 antibody (ab109764) at 0.5 µg + A431 cell lysate (in RIPA buffer) at 35 µgdeveloped using the ECL technique

Anti-DDX5 antibody - ab92917 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-DDX5 antibody (ab92917) at 1/500 dilution + Hela nuclear extract at 50 µg

Anti-DDX5 antibody - ab100873 from Abcam

Prices $378.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DDX5 antibody (ab100873) at 1/500 dilutionLane 1 : K562 cell lysateLane 2 : 293T cell lysate

Anti-DDX5 antibody - ab82415 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-DDX5 antibody (ab82415) at 1/50 dilution + T47D cell line lysates at 12.5 µg

Anti-DDX5 antibody - ab61154 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab61154 at 1/100 dilution staining HeLa cells without (left) and with (right) blocking peptide.

Anti-DDX5 antibody [2257C3a] - ab53216 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2257C3a
Anti-DDX5 antibody [2257C3a] (ab53216) at 1/100 dilution + F2408 whole cell lysate at 25 µgSecondaryanti-Mouse IgG at 1/2500 dilutiondeveloped using the ECL technique

Anti-DDX5 antibody [EPR7239] - ab126730 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7239
All lanes : Anti-DDX5 antibody [EPR7239] (ab126730) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : SH-SY5Y cell lysateLane 3 : A431 cell lysateLane 4 : HT29 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-DDX5 antibody [EPR7239] (Alexa Fluor® 405) - ab199461 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7239
ab199461 staining DDX5 in SKNSH cells. The cells were fixed with 4% formaldehyde (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199461 at 1/100 dilution (shown in blue) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-DDX5 antibody [EPR7239] (Alexa Fluor® 488) - ab199226 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7239
ab199226 staining DDX5 in SKNSH cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199226 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-DDX5 antibody [EPR7239] (Alexa Fluor® 647) - ab199460 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7239
ab199460 staining DDX5 in SKNSH cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199460 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-DDX5 antibody [EPR7240] - ab128928 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7240
All lanes : Anti-DDX5 antibody [EPR7240] (ab128928) at 1/1000 dilutionLane 1 : HT29 cell lysateLane 2 : SHSY5Y cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-DDX5 Antibody - LS-C64577 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-DDX5 Antibody (C-Terminus) - LS-C64580 from LifeSpan Bioscience

Prices $545.00
Sizes 20 µg
Host Rabbit
Clonality Polyclonal

Anti-DDX5 Antibody (aa544-593) - LS-C31325 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Jurkat Cell Lysate

Anti-DDX5 Antibody (aa448-614) - LS-C143815 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
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