SUPT5H
| Gene Symbol | SUPT5H |
|---|---|
| Entrez Gene | 6829 |
| Alt Symbol | SPT5, SPT5H, Tat-CT1 |
| Species | Human |
| Gene Type | protein-coding |
| Description | suppressor of Ty 5 homolog (S. cerevisiae) |
| Other Description | DRB sensitivity-inducing factor 160 kDa subunit|DRB sensitivity-inducing factor large subunit|DSIF large subunit|DSIF p160|Tat-cotransactivator 1 protein|hSPT5|transcription elongation factor SPT5 |
| Swissprots | Q99639 O43279 Q59G52 O00267 |
| Accessions | CCQ43204 EAW56896 EAW56897 EAW56898 EAW56899 EAW56900 O00267 AB000516 BAA24075 AB209257 BAD92494 AF040253 AAD02179 AF052165 AK091476 AK295137 BAG58160 AK296117 BAG58866 AK302954 BAG64109 AK303480 BAG64516 BC024203 AAH24203 BP199253 DB064517 DB097498 DQ896795 ABM87794 U56402 AAC51102 Y12790 CAA73326 XM_005259183 XP_005259240 XM_006723337 XP_006723400 NM_001111020 NP_001104490 NM_001130824 NP_001124296 NM_001130825 NP_001124297 NM_003169 NP_003160 |
| Function | Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptio |
| Subcellular Location | Nucleus {ECO:0000269|PubMed:10075709}. |
| Tissue Specificity | Ubiquitously expressed. {ECO:0000269|PubMed:10075709, ECO:0000269|PubMed:8975720}. |
SPT5 CRISPR/Cas9 KO Plasmid (h) - sc-401535 from Santa Cruz Biotechnology
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SPT5 HDR Plasmid (h) - sc-401535-HDR from Santa Cruz Biotechnology
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SPT5 Double Nickase Plasmid (h) - sc-401535-NIC from Santa Cruz Biotechnology
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SPT5 Double Nickase Plasmid (h2) - sc-401535-NIC-2 from Santa Cruz Biotechnology
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SPT5 CRISPR Activation Plasmid (h) - sc-401535-ACT from Santa Cruz Biotechnology
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SPT5 CRISPR Activation Plasmid (h2) - sc-401535-ACT-2 from Santa Cruz Biotechnology
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SPT5 Lentiviral Activation Particles (h) - sc-401535-LAC from Santa Cruz Biotechnology
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SPT5 Lentiviral Activation Particles (h2) - sc-401535-LAC-2 from Santa Cruz Biotechnology
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SPT5 siRNA (h) - sc-38440 from Santa Cruz Biotechnology
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SPT5 shRNA Plasmid (h) - sc-38440-SH from Santa Cruz Biotechnology
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SPT5 shRNA (h) Lentiviral Particles - sc-38440-V from Santa Cruz Biotechnology
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SUPT5H RNAi - H00006829-R01 from Novus Biologicals
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MISSION® esiRNA - EHU157911 from Sigma-Aldrich
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MISSION® esiRNA - EHU117581 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN1518 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND1518 from Sigma-Aldrich
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SUPT5H siRNA (Human) - CRH4668 from Cohesion Biosciences
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SUPT5H - MBS8218520 from MyBioSource
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shRNA set against Human SUPT5H (NM_003169.3) - SHH421028 from Creative biogene
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shRNA set against Human SUPT5H(NM_003169.3) - SHL048324 from Creative biogene
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Human SUPT5H siRNA - orb265170 from Biorbyt
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SUPT5H shRNA Plasmid (Human) - CHH4668 from Cohesion Biosciences
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SUPT5H miRNA 3'UTR clone - MiUTR1H-10070 from Creative biogene
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SUPT5H miRNA 3'UTR clone - MiUTR3H-04659 from Creative biogene
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SUPT5H miRNA 3'UTR clone - MiUTR3H-04660 from Creative biogene
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