Gene Symbol | ULK3 |
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Entrez Gene | 25989 |
Alt Symbol | - |
Species | Human |
Gene Type | protein-coding |
Description | unc-51 like kinase 3 |
Other Description | serine/threonine-protein kinase ULK3 |
Swissprots | D3DW68 Q6PHR2 B4DFT0 B2RXK3 B4DRQ7 Q9UFS4 Q9NPN5 |
Accessions | EAW99302 EAW99304 EAW99305 Q6PHR2 AI677802 AK075047 AK293181 BAG56723 AK293650 BAG57102 AK294241 BAG57537 AK294245 BAG57541 AK294251 BAG57547 AK298879 BAG60995 AK299380 BAG61369 AK316102 BAH14473 AL117482 CAB55955 AL360256 CAB96176 BC036117 BC048289 BC056423 BC157884 AAI57885 BI829133 BQ927030 XM_005254289 XP_005254346 XM_011521445 XP_011519747 XR_931789 XR_931790 XR_931791 XR_931792 NM_001099436 NP_001092906 NM_001284364 NP_001271293 NM_001284365 NP_001271294 NR_104307 NR_104308 NR_104309 |
Function | Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy. {ECO:0000269|PubMed:19279323, ECO:0000269|PubMed:19878745, ECO:0000269|PubMed:20643644}. |
Subcellular Location | Cytoplasm {ECO:0000269|PubMed:19279323, ECO:0000269|PubMed:19878745}. Note=Localizes to pre-autophagosomal structure during cellular senescence. |
Tissue Specificity | Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in the hippocampus, high levels also detected in the cerebellum, olfactory bulb and optic nerve. In the central nervous system, lowest levels in the spinal cord. {ECO:0000269|PubMed:19878745}. |
Top Pathways | Regulation of autophagy, mTOR signaling pathway |
ULK3 CRISPR/Cas9 KO Plasmid (h) - sc-403581 from Santa Cruz Biotechnology
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ULK3 HDR Plasmid (h) - sc-403581-HDR from Santa Cruz Biotechnology
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ULK3 Double Nickase Plasmid (h) - sc-403581-NIC from Santa Cruz Biotechnology
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ULK3 Double Nickase Plasmid (h2) - sc-403581-NIC-2 from Santa Cruz Biotechnology
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ULK3 CRISPR Activation Plasmid (h) - sc-403581-ACT from Santa Cruz Biotechnology
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ULK3 CRISPR Activation Plasmid (h2) - sc-403581-ACT-2 from Santa Cruz Biotechnology
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ULK3 Lentiviral Activation Particles (h) - sc-403581-LAC from Santa Cruz Biotechnology
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ULK3 Lentiviral Activation Particles (h2) - sc-403581-LAC-2 from Santa Cruz Biotechnology
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ULK3 siRNA (h) - sc-89940 from Santa Cruz Biotechnology
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ULK3 shRNA Plasmid (h) - sc-89940-SH from Santa Cruz Biotechnology
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ULK3 shRNA (h) Lentiviral Particles - sc-89940-V from Santa Cruz Biotechnology
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ULK3 RNAi - H00025989-R02 from Novus Biologicals
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MISSION® esiRNA - EHU040221 from Sigma-Aldrich
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MISSION® siRNA - SIHK2426 from Sigma-Aldrich
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MISSION® siRNA - SIHK2425 from Sigma-Aldrich
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MISSION® siRNA - SIHK2424 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN22487 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND22487 from Sigma-Aldrich
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ULK3 siRNA (Human) - CRH8637 from Cohesion Biosciences
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ULK3 - MBS8223407 from MyBioSource
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shRNA set against Human ULK3 (NM_001099436.1) - SHH440392 from Creative biogene
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Human ULK3 siRNA - orb269139 from Biorbyt
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ULK3 shRNA Plasmid (Human) - CHH8637 from Cohesion Biosciences
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ULK3 miRNA 3'UTR clone - MiUTR1H-11004 from Creative biogene
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