TIMELESS

Gene Symbol TIMELESS
Entrez Gene 8914
Alt Symbol TIM, TIM1, hTIM
Species Human
Gene Type protein-coding
Description timeless circadian clock
Other Description Tof1 homolog|protein timeless homolog|timeless circadian clock 1|timeless homolog
Swissprots Q8IWH3 Q86VM1 Q9UNS1 O94802 B2ZAV0
Accessions ACD11488 EAW96939 Q9UNS1 AB015597 BAA36499 AF098162 AAC80011 AK000721 AK022702 BAG51106 AK296869 BAG59432 AY207390 BC031514 BC039842 AAH39842 BC050557 AAH50557 BE503292 BP363571 BX640990 CAE45996 XM_011538939 XP_011537241 NM_003920 NP_003911
Function Plays an important role in the control of DNA replication, maintenance of replication fork stability, maintenance of genome stability throughout normal DNA replication and in the regulation of the circadian clock. Involved in the determination of period length and in the DNA damage-dependent phase advancing of the circadian clock. Negatively regulates CLOCK|NPAS2-ARTNL/BMAL1|ARTNL2/BMAL2-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus. Forms a complex with TIPIN and this complex regulates DNA replication processes under both normal and stress conditions, stabilizes replication forks and influences both CHEK1 phosphorylation and the intra-S phase checkpoint in response to genotoxic stress. Timeless promotes TIPIN nuclear localization. Involved in cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. May also play an importa
Subcellular Location Nucleus {ECO:0000269|PubMed:17141802}.
Tissue Specificity Expressed in all tissues examined including brain, heart, lung, liver, skeletal muscle, kidney, placenta, pancreas, spleen, thymus and testis. Highest levels of expression in placenta, pancreas, thymus and testis. {ECO:0000269|PubMed:9856465, ECO:0000269|PubMed:9891984}.

Timeless (A-3) - sc-393122 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Timeless (A-3): sc-393122. Western blot analysis of Timeless expression in HeLa (A) and Jurkat (B) nuclear extracts and RAW 264.7 (C) and NIH/3T3 (D) whole cell lysates.

Timeless (G-4) - sc-393146 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Timeless (G-4): sc-393146. Western blot analysis of Timeless expression in Jurkat (A) and HeLa (B) nuclear extracts and NIH/3T3 whole cell lysate (C).

Timeless (N-17) - sc-8891 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Timeless (N-17): sc-8891. Western blot analysis of Timeless expression in non-transfected: sc-117752 (A) and human Timeless transfected: sc-116208 (B) 293T whole cell lysates.

Timeless (A-17) - sc-12374 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Timeless (A-17): sc-12374. Western blot analysis of Timeless expression in HeLa (A) and Jurkat (B) nuclear extracts.

Timeless (H-300) - sc-367179 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Timeless (H-300): sc-367179. Western blot analysis of Timeless expression in non-transfected 293T: sc-117752 (A), human Timeless transfected 293T: sc-116208 (B) and PC-3 (C) whole cell lysates.

Anti-Timeless antibody - ab72458 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Timeless with ab72458 at 1/400 (0.5µg/ml). A DyLight® 594-conjugated goat anti-rabbit IgG (H+L) (1/100) was used as the secondary antibody.

Anti-Timeless antibody - ab84502 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Staining of Timeless by Immunohistochemistry on a formalin fixed, paraffin embedded section of human breast carcinoma using a 1/250 dilution of ab84502. Detection: DAB staining.

Anti-Timeless antibody [EPR5274(2)] - ab133569 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5274(2)
Flow cytometry analysis of permeabilized mouse NIH/3T3 cells staining Timeless using ab133569 at a 1/10 dilution (red) or a rabbit IgG control antibody (green).

Anti-Timeless antibody [EPR5275] - ab109512 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5275
All lanes : Anti-Timeless antibody [EPR5275] (ab109512) at 1/50000 dilutionLane 1 : HeLa cell lysateLane 2 : Jurkat cell lysateLane 3 : JAR cell lysateLane 4 : BxPC-3 cell lysateLysates/proteins at 10 µg per lane.

Anti-Timeless antibody - N-terminal - ab181672 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TIMELESS Antibody (N-Terminus) - LS-C10570 from LifeSpan Bioscience

Prices $415.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-TIMELESS Antibody (N-Terminus) - LS-C10582 from LifeSpan Bioscience

Prices $530.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-TIMELESS Antibody (C-Terminus) - LS-C92834 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-TIMELESS Antibody (N-Terminus) - LS-C145102 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-TIMELESS Antibody (aa1-230, clone RMT2-25, Low Endotoxin) - LS-C187573 from LifeSpan Bioscience

Prices $450.00
Sizes 500 µg
Host Rat
Clonality Monoclonal
Clone RMT2-25

Anti-TIMELESS Antibody (N-Terminus) - LS-C31481 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Transfected 293T Cell Lysate

Anti-TIMELESS Antibody (C-Terminus) - LS-C31480 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Transfected 293T Cell Lysate

Anti-TIMELESS Antibody (N-Terminus, clone EPR5274(2)) - LS-C138896 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5274(2)
Western blot analysis on (A) Jurkat, (B) HeLa, (C) JAR, (D) RAW 264.7, (E) PC-12, and (F) NIH/3T3 cell lysates using anti-Timeless antibody.

Anti-TIMELESS Antibody (aa1120-1170) - LS-C287794 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human Timeless by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Timeless used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.

Anti-TIMELESS Antibody (clone RMT2-25, FITC) - LS-C187574 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Rat
Clonality Monoclonal
Clone RMT2-25
TIM-2 transfected NRK cells stained with Rat anti-Mouse TIM-2:FITC

Anti-TIMELESS Antibody (C-Terminus) - LS-C287793 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human Timeless by Immunofluorescence. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Timeless used at a dilution of 1:400 (0.5 ug/ml). Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated (A120-601D4) used at a dilution of 1:100.

Anti-TIMELESS Antibody (C-Terminus, clone EPR5275) - LS-C138478 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5275
IHC of paraffin-embedded human tonsil tissue using anti-Timeless antibody.

Anti-TIMELESS Antibody (C-Terminus) - LS-C285922 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Mouse Timeless by Immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-Timeless used at a dilution of 1:250.

Anti-TIMELESS Antibody (aa290-339) IHC-plus™ - LS-B6788 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-TIMELESS antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6788 concentration 5 ug/ml.

Timeless Antibody - NB100-40853 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: Timeless Antibody [NB100-40853] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-Timeless used at a dilution of 1:100. Detection: Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG used at a dilution of 1:500.
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