NPL

Gene Symbol NPL
Entrez Gene 80896
Alt Symbol C112, C1orf13, NAL, NPL1
Species Human
Gene Type protein-coding
Description N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase)
Other Description N-acetylneuraminate lyase|N-acetylneuraminic acid aldolase|NALase|dihydrodipicolinate synthetase homolog 1|sialate-pyruvate lyase|sialic acid aldolase
Swissprots Q6PEL0 Q4G0Z2 Q4G0Q8 B2R839 Q9BXD5 Q64L88
Accessions EAW91132 EAW91133 EAW91134 EAW91135 Q9BXD5 AF338436 AAK25795 AI249145 AK297017 BAG59549 AK301068 BAG62675 AK313225 BAG36036 AL525867 AY336748 AAQ82432 BC034966 BC042003 BC058003 AAH58003 BC125051 AAI25052 CB961482 NM_001200050 NP_001186979 NM_001200051 NP_001186980 NM_001200052 NP_001186981 NM_001200056 NP_001186985 NM_030769 NP_110396
Function Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate. It prevents sialic acids from being recycled and returning to the cell surface. Involved in the N- glycolylneuraminic acid (Neu5Gc) degradation pathway. Although human is not able to catalyze formation of Neu5Gc due to the inactive CMAHP enzyme, Neu5Gc is present in food and must be degraded (By similarity). {ECO:0000250}.
Subcellular Location Cytoplasm {ECO:0000250}.
Tissue Specificity Isoform 2 is expressed in placenta, liver, kidney, pancreas, spleen, thymus, ovary, small intestine and peripheral blood leukocyte. {ECO:0000269|PubMed:16147865}.
Top Pathways Amino sugar and nucleotide sugar metabolism

NPL (C-19) - sc-243628 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

NPL (P-19) - sc-243627 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-NPL antibody - ab121981 from Abcam

Prices $443.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab121981, at a 1/15 dilution, staining Human NPL in adrenal gland, using Immunohistochemistry, paraffin-embedded tissue.

Anti-NPL antibody - N-terminal - ab139057 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-NPL antibody - N-terminal (ab139057) at 1/100 dilution + U251 cell line lysate at 35 µg

Anti-NPL / C112 Antibody (aa49-77, Azide-free, HRP) - LS-C223496 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NPL / C112 Antibody (aa49-77, PE) - LS-C223497 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NPL / C112 Antibody (aa49-77, AP) - LS-C226925 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NPL / C112 Antibody (aa49-77, APC) - LS-C226926 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NPL / C112 Antibody (aa49-77, FITC) - LS-C226928 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NPL / C112 Antibody (aa49-77, Biotin) - LS-C226927 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NPL / C112 Antibody (aa48-77) - LS-C323415 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NPL / C112 Antibody (aa1-237) - LS-C311895 from LifeSpan Bioscience

Prices $250.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NPL / C112 Antibody (aa49-77) - LS-C158417 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
NPL Antibody western blot of U251 cell line lysates (35 ug/lane). The NPL antibody detected the NPL protein (arrow).

Anti-NPL / C112 Antibody (aa61-110) - LS-C146312 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
NPL antibody LS-C146312 Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml.

NPL Antibody - NBP1-88482 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: NPL Antibody [NBP1-88482] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11<br/>Lane 2: Human cell line RT-4

Anti-NPL (216-230) antibody produced in rabbit - SAB1103183 from Sigma-Aldrich

Prices $298.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells was separated on SDS-PAGE, blotted with Anti-NPL (216-230) (<B>Cat. No. SAB1103183</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:1,000

Anti-NPL (287-297) antibody produced in rabbit - SAB1103184 from Sigma-Aldrich

Prices $298.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human NPL was separated on SDS-PAGE, blotted with Anti-NPL (287-297) (<B>Cat. No. SAB1103184</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:1,000 <BR/>2. Antibody dilution 1:1,000 + NPL immunizing peptide (human, 287-297)

Anti-NPL antibody produced in rabbit - HPA028345 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-NPL: <B>Cat. No. HPA028345</B>: Immunohistochemical staining of human adrenal gland shows distinct positivity in cortical cells.

NPL antibody - N-terminal region (ARP63210_P050) - ARP63210_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

NPL-Antibody-N-term - AP18910a from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
NPL Antibody (N-term)(Cat. #AP18910a) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the NPL antibody detected the NPL protein (arrow).

NPL antibody - GTX120940 from GeneTex

Prices $149.00
Sizes 100 μl
Host Rabbit
Clonality Polyclonal

NPL Antibody - N-terminal region (OAAB14448) - OAAB14448 from Aviva Systems Biology

Prices $289.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal

NPL antibody - 70R-18938 from Fitzgerald

Prices $375.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

NPL Antibody - abx030633 from Abbexa

Host Rabbit
Clonality Polyclonal

NPL antibody - 16715-1-AP from Acris Antibodies

Prices $270.00
Sizes 150 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of MCF-7 cells, using NPL antibody 16715-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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