DCTN3

Gene Symbol DCTN3
Entrez Gene 11258
Alt Symbol DCTN-22, DCTN22
Species Human
Gene Type protein-coding
Description dynactin 3 (p22)
Other Description dynactin complex subunit 22 kDa subunit|dynactin light chain|dynactin subunit 3
Swissprots Q5T8G3 Q5T1I5 A6NII7 Q5T1I7 Q9BPU8 B2RBM5 O75935
Accessions EAW58439 EAW58440 EAW58441 EAW58442 O75935 AF082513 AAC61280 AK055199 AK091661 BAC03716 AK314730 BAG37272 AW953496 BC000319 AAH00319 BC003004 AAH03004 BC107697 AAI07698 BG715167 BI596981 BI837796 CN257020 CR541889 CAG46687 DA212054 DQ891980 ABM82906 EU176665 ABW03466 NM_001281425 NP_001268354 NM_001281426 NP_001268355 NM_001281427 NP_001268356 NM_007234 NP_009165 NM_024348 NP_077324
Function Together with dynein may be involved in spindle assembly and cytokinesis. {ECO:0000269|PubMed:9722614}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:9722614}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:9722614}. Chromosome, centromere, kinetochore {ECO:0000269|PubMed:9722614}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:9722614}. Cleavage furrow {ECO:0000269|PubMed:9722614}. Midbody {ECO:0000269|PubMed:9722614}. Note=Localizes to punctate cytoplasmic structures and to the centrosome during interphase, and to kinetochores and to spindle poles throughout mitosis. Colocalizes with dynein to the cleavage furrow and to midbody of dividing cells.
Tissue Specificity Ubiquitously expressed. Highly expressed in muscle and pancreas and detected at lower levels in brain. {ECO:0000269|PubMed:9722614}.

Dynactin 3 (C-14) - sc-162754 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Dynactin 3 (D-14) - sc-162755 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Dynactin 3 (D-14): sc-162755. Western blot analysis of Dynactin 3 expression in rat skeletal muscle tissue extract.

Anti-DCTN3 antibody - ab168504 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-DCTN3 antibody (ab168504) at 1 µg/mlLane 1 : DCTN3 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.

Anti-DCTN3 antibody - ab106345 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DCTN3 antibody - ab67598 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-DCTN3 antibody (ab67598) at 1/500 dilutionLane 1 : DCTN3 transfected 293T cell lysateLane 2 : Non transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-DCTN3 antibody [EPR5097] - ab124674 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5097
All lanes : Anti-DCTN3 antibody [EPR5097] (ab124674) at 1/10000 dilution (purified)Lane 1 : 293T cell lysateLane 2 : SH-SY5Y cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-Dynactin 3 / DCTN3 Antibody (Internal) - LS-C145063 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-Dynactin 3 / DCTN3 Antibody (aa46-55) - LS-C154798 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-Dynactin 3 / DCTN3 Antibody - LS-C307193 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-Dynactin 3 / DCTN3 Antibody (aa41-52) - LS-C139670 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
DCTN3 antibody (0.03 ug/ml) staining of Human Kidney lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Dynactin 3 / DCTN3 Antibody (C-Terminus) - LS-C145824 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
DCTN3 antibody LS-C145824 Western blot of 293T Cell lysate. Antibody concentration 1 ug/ml.

Anti-Dynactin 3 / DCTN3 Antibody (Internal, clone EPR5097) - LS-C138732 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5097
IHC of paraffin-embedded human breast tissue using anti-DCTN3 antibody.

DCTN3 Antibody - NBP1-91823 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: DCTN3 Antibody [NBP1-91823] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

DCTN3 Antibody - H00011258-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: DCTN3 Antibody [H00011258-B01] - analysis of DCTN3 expression in transfected 293T cell line by DCTN3 MaxPab polyclonal antibody (B01). Lane 1: DCTN3 transfected lysate (19.50 KDa). Lane 2: Non-transfected lysate.

DCTN3 Antibody - H00011258-B01P from Novus Biologicals

Prices $349.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: DCTN3 Antibody [H00011258-B01P] - analysis of DCTN3 expression in transfected 293T cell line by DCTN3 MaxPab polyclonal antibody. Lane 1: DCTN3 transfected lysate (19.36 KDa). Lane 2: Non-transfected lysate.

DCTN3 Antibody - NBP1-52105 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: DCTN3 Antibody [NBP1-52105] - Analysis of DCTN3 in Human Kidney lysate (35ug protein in RIPA buffer) using NBP1-52105 at 0.03 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

DCTN3 Antibody - NBP2-16114 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: DCTN3 Antibody [NBP2-16114] - Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE gel, diluted at 1:500.

DCTN3 Polyclonal Antibody - bs-7746R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with DCTN3 Polyclonal Antibody (bs-7746R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.

Anti-DCTN3 antibody produced in goat - SAB2501357 from Sigma-Aldrich

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-DCTN3 <B>Cat. No. SAB2501357</B>: (0.03 μg/mL) staining of Human Kidney lysate (35 μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-DCTN3 antibody produced in rabbit - SAB2107711 from Sigma-Aldrich

Prices $341.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-DCTN3: <B>Cat. No. SAB2107711</B>: Western blot analysis of DCTN3 in 293T with DCTN3 (dynactin 3, p22) antibody, against the C terminal of DCTN3 (50 μg) antibody at 1.0 μg/mL.

Anti-DCTN3 antibody produced in mouse - SAB1400426 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Polyclonal Anti-DCTN3, antibody produced in mouse, purified immunoglobulin: <B>Cat. No. SAB1400426</B>: Antibody concentration: 1.0 μg/mL using HeLa cells.

Anti-DCTN3 antibody produced in rabbit - HPA044905 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-DCTN3: <B>Cat. No. HPA044905</B>: Immunohistochemical staining of human pancreas shows distinct cytoplasmic positivity in exocrine glandular cells.

DCTN3 antibody - C-terminal region (ARP62445_P050) - ARP62445_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

DCTN3-Antibody-internal-region - AF3366a from Abgent, a WuXi AppTec company

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
AF3366a (0.03 µg/ml) staining of Human Kidney lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

DCTN3-Antibody-internal-region - AF3365a from Abgent, a WuXi AppTec company

Prices $325.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
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