CHD8

Gene Symbol CHD8
Entrez Gene 57680
Alt Symbol AUTS18, HELSNF1
Species Human
Gene Type protein-coding
Description chromodomain helicase DNA binding protein 8
Other Description ATP-dependent helicase CHD8|axis duplication inhibitor|chromodomain-helicase-DNA-binding protein 8|duplin|helicase with SNF2 domain 1
Swissprots Q8NCY4 Q96F26 Q8TBR9 Q6ZNL7 Q9HCK8 Q6P440 Q4G0D8 Q68DQ0 Q6DKH9 Q8N3Z9
Accessions CCQ43659 EAW66385 Q9HCK8 AB046784 BAB13390 AK056639 AK098408 AK122868 AK122910 AK131077 BAC85127 AL834524 CAD39180 AW327785 BC011695 AAH11695 BC025964 AAH25964 BC036920 AAH36920 BC040636 BC063693 AAH63693 BC073903 AAH73903 BC098452 AAH98452 BC156439 AAI56440 CN290520 CR749315 CAH18170 DA327823 DB344386 U00930 NM_001170629 NP_001164100 NM_020920 NP_065971
Function DNA helicase that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1- targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating in efficient U6 RNA polymerase III transcription. {ECO:0000269|PubMed:17938208, ECO:0000269|PubMed:18378692}.
Subcellular Location Nucleus {ECO:0000269|PubMed:18378692, ECO:0000269|PubMed:20453063}. Note=Localizes to the promoter regions of several CTNNB1-responsive genes. Also present at known CTCF target sites.
Top Pathways Wnt signaling pathway

CHD8 Antibody - 7656 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from SH-SY5Y and HeLa cells using CHD8 Antibody.

CHD8 (D3C1) Rabbit mAb - 11891 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D3C1
Western blot analysis of extracts from OVCAR8 and SR cells using CHD8 (D3C1) Rabbit mAb.

CHD8 (C-16) - sc-104835 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

CHD8 (N-12) - sc-104836 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-CHD8 antibody - ab84527 from Abcam

Prices $390.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab84527, at 1/100 dilution, staining CHD8 in formalin-fixed, paraffin-embedded human breast carcinoma by Immunohistochemistry, using DAB staining.

Anti-CHD8 antibody - ab114126 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling CHD8 with ab114126 at 1/1000 (1µg/ml). Detection: DAB.

Anti-CHD8 Antibody (aa325-350) - LS-C79115 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-CHD8 Antibody (aa2252-2302) - LS-C79116 from LifeSpan Bioscience

Prices $545.00
Sizes 20 µg
Host Rabbit
Clonality Polyclonal

Anti-CHD8 Antibody (aa1980-2079) - LS-C306932 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-CHD8 Antibody (aa2252-2302) - LS-C288034 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human CHD8 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHD8 used at a dilution of 1:200 (1 ug/ml).

Anti-CHD8 Antibody (aa325-350) - LS-C288046 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human CHD8 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CHD8 used at a dilution of 1:1000 (1 Detection: DAB.

Anti-CHD8 Antibody (aa2252-2302) - LS-C286494 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Mouse CHD8 by Immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-CHD8 used at a dilution of 1:100.

CHD8 Antibody - NB100-60418 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: CHD8 Antibody [NB100-60418] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-60418 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. CHD8 was also immunoprecipitated byNB100-60417, which recognizes an upstream epitope.

CHD8 Antibody - NB100-60417 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry: CHD8 Antibody [NB100-60417] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CHD8 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

CHD8 Antibody (2C8) - H00057680-M11 from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2C8
Western Blot: CHD8 Antibody (2C8) [H00057680-M11] - Western Blot detection against Immunogen (36.63 KDa)

CHD8 Antibody - H00057680-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

CHD8 Antibody (4F2) - H00057680-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4F2

Monoclonal Anti-CHD8 antibody produced in mouse - SAB1405222 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4F2
<B>Immunoblotting</B><BR/>Monoclonal Anti-CHD8: <B>Cat. No. SAB1405222</B>: Antibody concentration: 1 μg/mL. Specific band of ~37 kDa using immunogen protein lysate.

Monoclonal Anti-CHD8 antibody produced in mouse - WH0057680M11 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2C8
<B>Immunoblotting</B><BR/>Monoclonal Anti-CHD8, <B>Cat. No. WH0057680M11</B> used at the antibody concentration: 1 μg/mL. Specific band of ~37 kDa using immunogen protein lysate.

CHD8 antibody - H00057680-A01 from Acris Antibodies

Prices $300.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

CHD8 antibody - H00057680-M01 from Acris Antibodies

Prices $360.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4F2

CHD8 antibody - H00057680-M01A from Acris Antibodies

Prices $360.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone 4F2
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