GCH1

Gene Symbol GCH1
Entrez Gene 2643
Alt Symbol DYT14, DYT5, DYT5a, GCH, GTP-CH-1, GTPCH1, HPABH4B
Species Human
Gene Type protein-coding
Description GTP cyclohydrolase 1
Other Description GTP cyclohydrolase I|GTP-CH-I|dystonia 14|guanosine 5'-triphosphate cyclohydrolase I
Swissprots Q9Y4I8 P30793 Q6FHY7
Accessions AAB42186 AAB60633 CAA83213 EAW80647 EAW80648 EAW80649 EAW80650 EAW80651 EAW80652 EAW80653 P30793 AF321276 AAK51704 AY137463 AAN17459 AY137464 AAN17460 AY137465 AAN17461 BC025415 AAH25415 BM971258 CR536551 CAG38788 DQ892998 ABM83924 DQ896245 ABM87244 S43856 AAB23166 S44049 AAB23164 S44053 AAB23165 U19523 AAB16861 U66095 AAD38866 U66097 AAD38868 Z16418 CAA78908 Z29433 CAB77391 Z29434 CAB77392 XM_005267530 XP_005267587 XM_011536643 XP_011534945 NM_000161 NP_000152 NM_001024024 NP_001019195 NM_001024070 NP_001019241 NM_001024071 NP_001019242
Function Positively regulates nitric oxide synthesis in umbilical vein endothelial cells (HUVECs). May be involved in dopamine synthesis. May modify pain sensitivity and persistence. Isoform GCH-1 is the functional enzyme, the potential function of the enzymatically inactive isoforms remains unknown. {ECO:0000269|PubMed:12176133, ECO:0000269|PubMed:16338639, ECO:0000269|PubMed:17057711, ECO:0000269|PubMed:8068008, ECO:0000269|PubMed:9445252}.
Subcellular Location Cytoplasm. Nucleus.
Tissue Specificity In epidermis, expressed predominantly in basal undifferentiated keratinocytes and in some but not all melanocytes (at protein level). {ECO:0000269|PubMed:16778797}.
Top Pathways Folate biosynthesis

GCH-I (C-4) - sc-271482 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GCH-I (C-4): sc-271482. Western blot analysis of GCH-I expression in non-transfected: sc-117752 (A) and mouse GCH-I transfected: sc-120450 (B) 293T whole cell lysates.

GCH-I (FL-250) - sc-134574 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GCH-I (FL-250): sc-134574. Western blot analysis of GCH-I expression in non-transfected: sc-117752 (A) and mouse GCH-I transfected: sc-120450 (B) 293T whole cell lysates.

GCH-I (G-8) - sc-376483 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GCH-I (G-8): sc-376483. Western blot analysis of GCH-I expression in non-transfected 293T: sc-117752 (A), human GCH-I transfected 293T: sc-159287 (B) and IMR-32 (C) whole cell lysates.

GCH-I (K-15) - sc-48510 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GCH-I (K-15): sc-48510. Western blot analysis of GCH-I expression in non-transfected: sc-117752 (A) and mouse GCH-I transfected: sc-120450 (B) 293T whole cell lysates.

Anti-GTP cyclohydrolase 1 antibody - ab61858 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab61858 stained PANC-1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab61858 at 5 ug/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GTP cyclohydrolase 1 antibody - ab106826 from Abcam

Prices $370.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-GTP cyclohydrolase 1 antibody (ab106826) at 1 µg/ml + Human thymus lysate in RIPA buffer at 35 µgdeveloped using the ECL technique

Anti-GTP cyclohydrolase 1 antibody - ab69962 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-GTP cyclohydrolase 1 antibody (ab69962) at 1/500 dilutionLane 1 : GCH1 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-GTP cyclohydrolase 1 antibody - ab171920 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GTP cyclohydrolase 1 antibody (ab171920) at 1 µg/mlLane 1 : GTP cyclohydrolase 1-transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Rabbit IgG (H+L) at 1/7500 dilutiondeveloped using the ECL technique

Anti-GTP cyclohydrolase 1 antibody - N-terminal - ab186633 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-GTP cyclohydrolase 1 antibody - N-terminal (ab186633) at 1 µg/ml + Human fetal heart tissue lysate at 10 µgdeveloped using the ECL technique

Anti-GTPCH1 / GCH1 Antibody (clone 4A12) - LS-C133216 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4A12
Western blot of GCH1 expression in transfected 293T cell line by GCH1 monoclonal antibody (M01), clone 4A12.

Anti-GTPCH1 / GCH1 Antibody (aa150-161) - LS-C87242 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
GCH1 antibody (1 ug/ml) staining of Human Thymus lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-GTPCH1 / GCH1 Antibody (aa39-67, AP) - LS-C235572 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GTPCH1 / GCH1 Antibody (aa39-67, APC) - LS-C235574 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GTPCH1 / GCH1 Antibody (C-Terminus) - LS-C135566 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
GCH1 antibody LS-C135566 Western blot of COLO205 cell lysate.

Anti-GTPCH1 / GCH1 Antibody (aa39-67, Biotin) - LS-C235575 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GTPCH1 / GCH1 Antibody (aa39-67, FITC) - LS-C235576 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GTPCH1 / GCH1 Antibody (aa39-67, PE) - LS-C235578 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GTPCH1 / GCH1 Antibody (aa39-67, Azide-free, HRP) - LS-C249046 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GTPCH1 / GCH1 Antibody (aa84-173, Azide-free) - LS-C327499 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-GTPCH1 / GCH1 Antibody (aa46-75) - LS-C321578 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GTPCH1 / GCH1 Antibody - LS-C307657 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-GTPCH1 / GCH1 Antibody (aa14-250) - LS-C311505 from LifeSpan Bioscience

Prices $250.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-GTPCH1 / GCH1 Antibody - LS-C311166 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GTPCH1 / GCH1 Antibody (aa39-88) - LS-C205829 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
GTPCH1 / GCH1 antibody Western Blot of Fetal Heart. Antibody dilution: 1 ug/ml.

Anti-GTPCH1 / GCH1 Antibody (aa39-67) - LS-C166411 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
GCH1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human pancreas followed by peroxidase-conjugated secondary antibody and DAB staining.
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