Cd8a

Gene Symbol Cd8a
Entrez Gene 12525
Alt Symbol BB154331, Ly-2, Ly-35, Ly-B, Lyt-2
Species Mouse
Gene Type protein-coding
Description CD8 antigen, alpha chain
Other Description Lyt-2.1 lymphocyte differentiation antigen (AA at 100)|T-cell surface glycoprotein CD8 alpha chain|T-cell surface glycoprotein Lyt-2
Swissprots P01731
Accessions AAA39474 AAA39475 AAA39665 CAA68352 EDK98938 EDK98939 P01731 AJ131778 AK017889 AK037429 AK037467 BAC29812 AK088128 BAC40163 AK088214 BAC40215 AK088414 BAC40342 AK088447 AK137937 BAE23516 BC030679 AAH30679 M12052 AAA39478 M12825 AAA39476 M16981 AAA39477 U34881 AAA92533 XM_006505464 XP_006505527 XM_006505465 XP_006505528 NM_001081110 NP_001074579 NM_009857 NP_033987
Function Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Subcellular Location Membrane; Single-pass type I membrane protein.
Top Pathways Hematopoietic cell lineage, T cell receptor signaling pathway, Cell adhesion molecules (CAMs), Primary immunodeficiency, Antigen processing and presentation

CD8α (53-6.7) Rat mAb - 13989 from Cell Signaling Technology

Prices $239.00
Sizes 100 µl (100 tests)
Host Rat
Clonality Monoclonal
Clone 53-6.7
Flow cytometric analysis of fixed murine splenocytes using CD8α (53-6.7) Rat mAb co-stained with CD4-APC, showing a distinct CD8α positive population with no cross reactivity on CD4 positive cells. A mouse anti-rat IgG2a PE conjugate was used as a secondary antibody.

CD8 (2.43) - sc-18860 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rat
Clonality Monoclonal
CD8 (2.43) FITC: sc-18860 FITC. FCM analysis of mouse peripheral blood leukocytes. Quadrant markers were set based on the isotype control, normal rat IgG<sub>2b</sub>: sc-2835.

CD8-α (53-6.7) - sc-18913 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rat
Clonality Monoclonal
CD8-α (53-6.7) FITC: sc-18913 FITC. FCM analysis of mouse peripheral blood leukocytes. Quadrant markers were set based on the isotype control, normal rat IgG<sub>2a</sub>: sc-2831.

CD8-α (KT15) - sc-53473 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rat
Clonality Monoclonal

CD8-α (R-15) - sc-1142 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-CD8 alpha antibody [53-6.7] - ab25478 from Abcam

Prices $388.00
Sizes 100 µg
Host Rat
Clonality Monoclonal
Clone 53-6.7
ab25478 staining CD8 alpha in mouse spleen tissue by Immunohistochemistry (frozen sections). Tissue was fixed with acetone and then blocked with 5% serum for 1 hour at 25°C followed by incubation with the primary antibody at a 1/250 dilution for 12 hours at 4°C. A goat anti-rat IgG conjugated to Alexa Fluor®594 was used as secondary antibody at a 1/250 dilution.See Abreview

Anti-CD8 alpha antibody [53-6.7] (Alexa Fluor® 488) - ab187774 from Abcam

Prices $370.00
Sizes 50 µg
Host Rat
Clonality Monoclonal
Clone 53-6.7

Anti-CD8 alpha antibody [53-6.7] (Allophycocyanin) - ab25499 from Abcam

Prices $373.00
Sizes 50 µg
Host Rat
Clonality Monoclonal
Clone 53-6.7

Anti-CD8 alpha antibody [53-6.7] (Allophycocyanin/Cy5.5 ® ) - ab82005 from Abcam

Prices $189.00
Sizes 50 µg
Host Rat
Clonality Monoclonal
Clone 53-6.7

Anti-CD8 alpha antibody [53-6.7] (Allophycocyanin/Cy7 ®) - ab25456 from Abcam

Prices $372.00
Sizes 50 µg
Host Rat
Clonality Monoclonal
Clone 53-6.7

Anti-CD8 alpha antibody [53-6.7] (Biotin) - ab25176 from Abcam

Prices $372.00
Sizes 100 µg
Host Rat
Clonality Monoclonal
Clone 53-6.7

Anti-CD8 alpha antibody [53-6.7] (Cy5 ®) - ab39850 from Abcam

Prices $372.00
Sizes 50 tests
Host Rat
Clonality Monoclonal
Clone 53-6.7

Anti-CD8 alpha antibody [53-6.7] (FITC) - ab25676 from Abcam

Prices $189.00
Sizes 50 µg
Host Rat
Clonality Monoclonal
Clone 53-6.7
Cells from BALB/c mesenteric lymph nodes were double stained with ab25676 (1µg for 106 cells) and antimouse CD4 PE conjugated, gated on small lymphocytes and analyzed on a flow cytometer.

Anti-CD8 alpha antibody [53-6.7] (PE/Cy5®) - ab95588 from Abcam

Prices $191.00
Sizes 50 µg
Host Rat
Clonality Monoclonal
Clone 53-6.7
Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.06 µg of ab95588 (filled histogram). Total cells were used for analysis.

Anti-CD8 alpha antibody [53-6.7] (PE/Cy5.5 ®) - ab37928 from Abcam

Prices $372.00
Sizes 50 µg
Host Rat
Clonality Monoclonal
Clone 53-6.7

Anti-CD8 alpha antibody [53-6.7] (PE/Cy7 ®) - ab39853 from Abcam

Prices $373.00
Sizes 50 tests
Host Rat
Clonality Monoclonal
Clone 53-6.7

Anti-CD8 alpha antibody [53-6.7] (PerCP) - ab107599 from Abcam

Prices $370.00
Sizes 50 µg
Host Rat
Clonality Monoclonal
Clone 53-6.7

Anti-CD8 alpha antibody [53-6.7] (Phycoerythrin) - ab25498 from Abcam

Prices $190.00
Sizes 50 µg
Host Rat
Clonality Monoclonal
Clone 53-6.7
BALB/c mesenteric lymph node cells were first labeled with anti Integrin alpha E (ab25211)  at 0.1 µg/106 cells, and then double-stained with streptavidin-FITC and ab25498.  Small lymphocytes were gated and analyzed on a FACScan flow cytometer  (BDIS, San Jose, CA).

Anti-CD8 alpha antibody [53-6.7] (Phycoerythrin -Texas Red®) - ab25294 from Abcam

Prices $189.00
Sizes 50 µg
Host Rat
Clonality Monoclonal
Clone 53-6.7

Anti-CD8 alpha antibody [53-6.7] - Low endotoxin, Azide free - ab174591 from Abcam

Prices $370.00
Sizes 100 µg
Host Rat
Clonality Monoclonal
Clone 53-6.7

Anti-CD8 alpha antibody [IBL-3/25] - ab3081 from Abcam

Prices $373.00
Sizes 100 µg
Host Rat
Clonality Monoclonal
Clone IBL-3/25

Anti-CD8 alpha antibody [KT15] (FITC) - ab22504 from Abcam

Prices $185.00
Sizes 50 tests
Host Rat
Clonality Monoclonal
Clone KT15

Anti-CD8 antibody [YTS169.4] - ab22378 from Abcam

Prices $400.00
Sizes 100 µg
Host Rat
Clonality Monoclonal
Clone YTS169.4
ab22378 staining CD8 in murine bone marrow cells by ICC/IF (immunocytochemistry/immunofluorescence). Cells were formaldehyde fixed prior to blocking in 2% BSA  for 30 minutes at 20°C. The primary antibody (1/200) was incubated with the sample for 9 hours at 4°C. An undiluted Alexa Fluor 555®-conjugated goat anti-rat polyclonal was used as the secondary. The nuclei were stained with DAPI.See Abreview

Anti-CD8alpha antibody [AD4(15)] (FITC) - ab112164 from Abcam

Prices $188.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone AD4(15)
Cell Source: Thymus Percentage of cells stained above control: 66.0%, ab112164 at 2.0 µg/106 cells

Anti-CD8alpha antibody [YTS 169.4] (Biotin) - ab112195 from Abcam

Prices $185.00
Sizes 50 µg
Host Rat
Clonality Monoclonal
Clone YTS 169.4
Search more