USP18

Gene Symbol USP18
Entrez Gene 11274
Alt Symbol ISG43, UBP43
Species Human
Gene Type protein-coding
Description ubiquitin specific peptidase 18
Other Description 43 kDa ISG15-specific protease|ISG15-specific-processing protease|hUBP43|ubiquitin specific protease 18|ubl carboxyl-terminal hydrolase 18|ubl thioesterase 18|ubl thiolesterase 18
Swissprots Q53Y90 Q6IAD9 Q9NY71 Q9UMW8
Accessions EAW57790 EAW57791 Q9UMW8 AF176642 AAD49967 AI222587 AJ243526 CAB76398 AK313385 BAG36183 AL136690 CAB66625 AM393395 CAL38273 BC014896 AAH14896 BI752849 BT006835 AAP35481 BU584005 CR457216 CAG33497 CT841507 CAJ86437 CU013072 CAK54503 CU013360 CAK54802 XM_006724074 XP_006724137 NM_017414 NP_059110
Function Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms.
Subcellular Location Isoform 1: Cytoplasm.

USP18 (D4E7) Rabbit mAb - 4813 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D4E7
Western blot analysis of extracts from THP-1 cells, untreated or LPS-treated (1 µg/ml, 24 hours), using USP18 (D4E7) Rabbit mAb.

USP18 (E-5) - sc-374064 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
USP18 (E-5): sc-374064. Western blot analysis of USP18 expression in non-transfected (A) and human USP18 transfected (B) CMV whole cell lysates.

USP18 (H-300) - sc-98431 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
USP18 (H-300): sc-98431. Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear staining of cells in seminiferous ducts and Leydig cells.

USP18 (S-20) - sc-50017 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-UBP43 antibody - ab115618 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab115618, at 5 µg/ml, staining UBP43 in Formalin-fixed, Paraffin-embedded Human heart tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-UBP43 antibody - ab168478 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-UBP43 antibody (ab168478) at 1 µg/mlLane 1 : UBP43 transfected 293T cell line lysateLane 2 : Non-transfected 293T cell line lysateLysates/proteins at 15 µl per lane.

Anti-UBP43 antibody - ab137417 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-UBP43 antibody (ab137417) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg

Anti-UBP43 antibody - ab80815 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-UBP43 antibody (ab80815) at 1 µg/ml + HeLa cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-UBP43 antibody - ab4188 from Abcam

Prices $242.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot using ab4188 at 1/500. Lane 1: Hela nuclear Lane 2: Hela Lane 3: A431 Lane 4: Jurkat Lane 5: HEK293 Secondary ab: Goat polyclonal to Rabbit IgG H&L (IR680) Pre-Adsorbed (1/5000) 18µg of cell lysate per lane. Expected molecular weight: 43 kD. The strong band between the 37kD and 50kD markers is expected to be UBP43, however, we are not yet certain of this. The other bands are probably non-specific.

Anti-UBP43 / USP18 Antibody - LS-C132833 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal

Anti-UBP43 / USP18 Antibody (aa110-159) - LS-C80869 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Hela cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-UBP43 / USP18 Antibody (aa40-49) IHC-plus™ - LS-B1182 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-USP18 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1182 concentration 5 ug/ml.

USP18 Antibody - NBP1-92566 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

USP18 Antibody - NBP1-33675 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

USP18 Antibody - NB600-893 from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: USP18 Antibody [NB600-893] - Analysis of a band 43 kDa band (arrowhead) believed to be to UBP43 in lysates from HeLa nuclear extracts (lane 1) and whole cell lysates from HeLa (lane 2), A431 (lane 3, Jurkat (Lane 4) and HEK293 (lane 5). Approximately 18 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-UBP43 antibody. Signal was detected using standard techniques. The strong band between the 37kD and 50kD markers is expected to be UBP43. The other bands are probably non-specific.

USP18 Antibody - H00011274-B02 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: USP18 Antibody [H00011274-B02] - analysis of USP18 expression in transfected 293T cell line by USP18 MaxPab polyclonal antibody (B02). Lane 1: USP18 transfected lysate (43.00 KDa). Lane 2: Non-transfected lysate.

USP18 Antibody - H00011274-B02P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: USP18 Antibody [H00011274-B02P] - Analysis of USP18 expression in transfected 293T cell line by USP18 polyclonal antibody. Lane 1: USP18 transfected lysate(40.92 KDa). Lane 2: Non-transfected lysate.

USP18 Antibody - H00011274-B03P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: USP18 Antibody [H00011274-B03P] - Analysis of USP18 expression in transfected 293T cell line. Lane 1: USP18 transfected lysate (40.92 KDa). Lane 2: Non-transfected lysate.

USP18 Antibody - H00011274-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: USP18 Antibody [H00011274-B01] - analysis of USP18 expression in transfected 293T cell line by USP18 MaxPab polyclonal antibody (B01). Lane 1: USP18 transfected lysate (40.66 KDa). Lane 2: Non-transfected lysate.

USP18 Antibody - H00011274-D01 from Novus Biologicals

Prices $399.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: USP18 Antibody [H00011274-D01] - Analysis of USP18 expression in transfected 293T cell line by USP18 polyclonal antibody.Lane 1: USP18 transfected lysate(40.92 KDa).Lane 2: Non-transfected lysate.

USP18 Antibody - H00011274-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: USP18 Antibody [H00011274-D01P] - Analysis of USP18 expression in transfected 293T cell line by USP18 polyclonal antibody.Lane 1: USP18 transfected lysate(40.92 KDa).Lane 2: Non-transfected lysate.

Anti-USP18 antibody produced in rabbit - SAB1410177 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-USP18: <B>Cat. No. SAB1410177</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~40.9 kDa using USP18 transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-USP18 antibody produced in rabbit - SAB2900247 from Sigma-Aldrich

Prices $464.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-USP18: <B>Cat. No.SAB2900247</B>: Heart: Formalin-Fixed Paraffin-Embedded (FFPE).

Anti-USP18 antibody produced in rabbit - SAB2102661 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-USP18: <B>Cat. No.SAB2102661</B>: USP18 in HeLa cell lysate with USP18 antibody at 1.0 μg/mL.

Anti-USP18 antibody produced in mouse - SAB1407126 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-USP18: <B>Cat. No. SAB1407126</B>: Antibody concentration: 10 μg/mL using HeLa cell.
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