BACE1

Gene Symbol BACE1
Entrez Gene 23621
Alt Symbol ASP2, BACE, HSPC104
Species Human
Gene Type protein-coding
Description beta-site APP-cleaving enzyme 1
Other Description APP beta-secretase|asp 2|aspartyl protease 2|beta-secretase 1|beta-secretase 1 precursor variant 1|beta-site APP cleaving enzyme 1|beta-site amyloid beta A4 precursor protein-cleaving enzyme|memapsin-2|membrane-associated aspartic protease 2|transmembrane aspartic proteinase Asp2
Swissprots Q9UJT5 Q9BYB9 E9PE65 P56817 B0YIU9 Q9ULS1 Q9BYC0 Q9BYC1 A0M8W7 H7BXJ9
Accessions AAO23912 AAY16982 ACA05927 CAI61888 EAW67308 EAW67309 EAW67310 EAW67311 EAW67312 EAW67313 EAW67314 EAW67315 EAW67316 EAW67317 P56817 AB032975 BAA86463 AB050436 BAB40931 AB050437 BAB40932 AB050438 BAB40933 AB089958 BAC81826 AB177847 BAD66825 AF161367 AAF28927 AF190725 AAF04142 AF200193 AAF13715 AF200343 AAF17079 AF201468 AAF18982 AF204943 AAF26367 AF324837 AAM54050 AF338816 AAK38374 AF338817 AAK38375 AF527782 AAM92013 AK075049 BAG52056 AK296015 BAH12238 AK296535 BAH12380 AL833810 CAD38674 BC036084 AAH36084 BC065492 AAH65492 BM996673 BQ005204 DC343475 DQ891509 ABM82435 DQ894700 ABM85626 NM_001207048 NP_001193977 NM_001207049 NP_001193978 NM_012104 NP_036236 NM_138971 NP_620427 NM_138972 NP_620428 NM_138973 NP_620429
Function Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. {ECO:0000269|PubMed:10677483, ECO:0000269|PubMed:20354142}.
Subcellular Location Membrane; Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Cytoplasmic vesicle membrane. Note=Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface.
Tissue Specificity Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata. {ECO:0000269|PubMed:10677483, ECO:0000269|PubMed:11083922}.
Top Pathways Alzheimer's disease

BACE (D10E5) Rabbit mAb - 5606 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D10E5
Western blot analysis of extracts from HeLa cells, untransfected and BACE transfected, and rat brain extracts using BACE (D10E5) Rabbit mAb.

BACE (A-12) - sc-365948 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
BACE (A-12): sc-365948. Western blot analysis of BACE expression in non-transfected: sc-117752 (A) and human BACE transfected: sc-159912 (B) 293T whole cell lysates.

BACE (61-3E7) - sc-33711 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
BACE (61-3E7): sc-33711. Western blot analysis of BACE expression in SH-SY5Y whole cell lysate.

BACE (H-10) - sc-365947 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
BACE (H-10): sc-365947. Western blot analysis of BACE expression in non-transfected: sc-117752 (A) and human BACE transfected: sc-159912 (B) 293T whole cell lysates.

BACE (D-16) - sc-10053 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
BACE (D-16): sc-10053. Western blot analysis of human recombinant BACE fusion protein.

BACE (C-15) - sc-10055 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
BACE (C-15): sc-10055. Western blot analysis of BACE expression in IMR-32 whole cell lysate.

BACE (M-83) - sc-10748 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
BACE (M-83): sc-10748. Western blot analysis of human recombinant BACE fusion protein.

Anti-BACE1 antibody - ab2077 from Abcam

Prices $392.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-BACE1 antibody (ab2077) at 1 µg/mlLane 1 : Human brain tissue lysate with absence of blocking peptideLane 2 : Human brain tissue lysate with BACE1 peptide (ab7883)Lane 3 : Mouse 3T3 cell lysateObserved band size : 70 kDa (why is the actual band size different from the predicted?)

Anti-BACE1 antibody - ab63954 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-BACE1 antibody (ab63954) at 1 µg/mlLane 1 : Lung (Rat) Tissue LysateLane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 3 : Kidney (Mouse) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-BACE1 antibody - ab62034 from Abcam

Host Rabbit
Clonality Polyclonal
ab62034 at 3µg/ml staining BACE1 in Human Alzheimer brain by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.

Anti-BACE1 antibody - ab11028 from Abcam

Prices $359.00
Sizes 50 µl
Host Goat
Clonality Polyclonal

Anti-BACE1 antibody - ab76019 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-BACE1 antibody (ab76019) at 1/50 dilution + Mouse cerebellum tissue lysates at 35 µg

Anti-BACE1 antibody - ab62048 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab62048 at 2.5µg/ml staining BACE1 in human pancreas by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.

Anti-BACE1 antibody - ab38892 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-BACE1 antibody [EPR3956] - ab108394 from Abcam

Prices $381.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3956
All lanes : Anti-BACE1 antibody [EPR3956] (ab108394) at 1/1000 dilutionLane 1 : Fetal brain lysateLane 2 : SH-SY5Y lysateLane 3 : Mouse brain lysateLane 4 : Rat brain lysateLysates/proteins at 10 µg per lane.

Anti-BACE1 antibody [MM0105-6J26] - ab89137 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone MM0105-6J26
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placental tissue section (4µm) labelling BACE1 with ab89137. Tissue was treated with 15µg/ml Protenase for antigen retrieval and fixed with 4% PFA. A HRP-conjugated anti-mouse IgG was used as the secondary antibody (30 minutes at room temperature) followed by DAB color development.

Anti-BACE1 antibody - Catalytic domain - ab38891 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab38891 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38891, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-BACE1 antibody - Cytoplasmic domain - ab38893 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-BACE1 antibody - N-terminal - ab79921 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-BACE1 antibody - N-terminal (ab79921) at 1/50 dilution + Mouse heart tissue lysate at 35 µg

Anti-BACE1 / BACE Antibody (clone 61-3E7) - LS-C180189 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 61-3E7

Anti-BACE1 / BACE Antibody (C-Terminus) IHC-plus™ - LS-A8549 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-BACE1 antibody LS-A8549 IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-BACE1 / BACE Antibody (aa72-121) IHC-plus™ - LS-B4664 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-BACE1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4664 concentration 5 ug/ml.

Anti-BACE1 / BACE Antibody - LS-C193929 from LifeSpan Bioscience

Prices $905.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-BACE1 / BACE Antibody - LS-C155223 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE BACE1 antibody diluted at 1:1000

Anti-BACE1 / BACE Antibody (Ser498, AP) - LS-C275348 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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