DSG3

Gene Symbol DSG3
Entrez Gene 1830
Alt Symbol CDHF6, PVA
Species Human
Gene Type protein-coding
Description desmoglein 3
Other Description 130 kDa pemphigus vulgaris antigen|130-kD pemphigus vulgaris antigen|cadherin family member 6|desmoglein-3|pemphigus vulgaris antigen
Swissprots P32926 A8K2V2
Accessions CAJ46922 EAX01258 EAX01259 P32926 AI608626 AK290367 BAF83056 BC063544 BC105997 BX110234 BX507120 BX538327 CAD98098 DA447747 DA458544 M76482 AAA60230 U51696 XM_011525850 XP_011524152 NM_001944 NP_001935
Function Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
Subcellular Location Cell membrane {ECO:0000250}; Single-pass type I membrane protein {ECO:0000250}. Cell junction, desmosome {ECO:0000250}.
Tissue Specificity Epidermis, tongue, tonsil, esophagus and carcinomas.

dsg3 (5G11) - sc-53487 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
dsg3 (5G11): sc-53487. Western blot analysis of dsg3 expression in SCC-25 whole cell lysate.

dsg3 (5H10) - sc-23912 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
dsg3 (5H10): sc-23912. Western blot analysis of dsg3 expression in SCC-25 whole cell lysate.

dsg3 (C-16) - sc-14867 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
dsg3 (C-16): sc-14867. Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing membrane and cytoplasmic staining of squamous epithelial cells.

dsg3 (H-145) - sc-20116 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
dsg3 (H-145): sc-20116. Western blot analysis of dsg3 expression in SCC-4 (A), U-698-M (B) and HeLa (C) whole cell lysates.

dsg3 (I-16) - sc-14866 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

dsg3 (3G133) - sc-59776 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
dsg3 (3G133): sc-59776. Western blot analysis of dsg3 expression in SCC-25 whole cell lysate.

Anti-Desmoglein 3 antibody - ab78448 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Desmoglein 3 antibody (ab78448) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Desmoglein 3 antibody [3G133] - ab14416 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3G133
IHC image of Desmoglein 3 staining in Human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab14416, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Desmoglein 3 antibody [7B9] - ab125710 from Abcam

Prices $379.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 7B9
Overlay histogram showing A431 cells stained with ab125710 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab125710, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed A431 cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-Desmoglein 3 antibody [EPR14101] - ab183743 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR14101
Immunohistochemical analysis of Human esophagus, staining Desmoglein 3 with ab183743 at 1/250 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

Anti-Desmoglein 3 antibody [EPR7420] - ab128927 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7420
All lanes : Anti-Desmoglein 3 antibody [EPR7420] (ab128927) at 1/1000 dilutionLane 1 : Human tonsil lysateLane 2 : A431 lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-Desmoglein 3 antibody [MM0245- 4W25] - ab89177 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone MM0245- 4W25
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placental tissue section (4µm) labelling Desmoglein 3 with ab89177. Tissue was treated with 15µg/ml Protenase K for antigen retrieval and fixed with 4% PFA. A HRP-conjugated anti-mouse IgG was used as the secondary antibody (30 minutes at room temperature) followed by DAB color development.

Anti-Desmoglein 3 antibody - C-terminal - ab191044 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Desmoglein 3 antibody - C-terminal (ab191044) at 0.5 µg/mlLane 1 : A431 cell lysateLane 2 : CEM cell lysate

Anti-DSG3 / Desmoglein 3 Antibody - LS-C44528 from LifeSpan Bioscience

Prices $580.00
Sizes 200 µg
Host Mouse
Clonality Monoclonal

Anti-DSG3 / Desmoglein 3 Antibody - LS-C149923 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-DSG3 / Desmoglein 3 Antibody (clone 5H10) - LS-C150722 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5H10

Anti-DSG3 / Desmoglein 3 Antibody (clone 5G11, Biotin) - LS-C180269 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5G11

Anti-DSG3 / Desmoglein 3 Antibody (clone 5G11, DY650) - LS-C180270 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5G11

Anti-DSG3 / Desmoglein 3 Antibody (clone 5G11, HRP) - LS-C180272 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5G11

Anti-DSG3 / Desmoglein 3 Antibody (clone 5G11, DY488) - LS-C180271 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5G11

Anti-DSG3 / Desmoglein 3 Antibody (clone 5H10, Biotin) - LS-C180966 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5H10

Anti-DSG3 / Desmoglein 3 Antibody (clone 5G11, DY550) - LS-C180273 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5G11

Anti-DSG3 / Desmoglein 3 Antibody (clone 5H10, DY650) - LS-C180967 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5H10

Anti-DSG3 / Desmoglein 3 Antibody (aa50-615, clone 216519) - LS-C149924 from LifeSpan Bioscience

Prices $385.00
Sizes 500 µg
Host Mouse
Clonality Monoclonal
Clone 216519
Desmoglein-3 in Human Squamous Cell Carcinoma. Desmoglein-3 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human Desmoglein-3 Monoclonal Antibody at 8 ug/ml overnight at 4°C. Tissue was stained using Anti-Mouse HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of keratinocytes.

Anti-DSG3 / Desmoglein 3 Antibody (aa854-903) - LS-C135953 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
DSG3 antibody LS-C135953 Western blot of HepG2 Cell lysate. Antibody concentration 1 ug/ml.
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