NPPA

Gene Symbol NPPA
Entrez Gene 4878
Alt Symbol ANF, ANP, ATFB6, ATRST2, CDD, CDD-ANF, CDP, PND
Species Human
Gene Type protein-coding
Description natriuretic peptide A
Other Description atriopeptin|cardiodilatin|cardiodilatin-related peptide|cardionatrin|natriuretic peptide precursor A|natriuretic peptides A|prepronatriodilatin
Swissprots Q5JZE1 P01160 Q13766
Accessions AAA35528 AAA35529 AAB59379 ACA05916 CAA25699 CAA25700 EAW71717 P01160 AA779538 BC005893 AAH05893 DQ893115 ABM84041 DQ896389 ABM87388 EU176780 ABW03581 K02044 AAA51730 M30262 AAA35669 NM_006172 NP_006163
Function Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. {ECO:0000269|PubMed:1672777}.
Subcellular Location Secreted.

ANP (FL-153) - sc-20158 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

ANP (N-20) - sc-18811 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ANP (N-20): sc-18811. Western blot analysis of ANP expression in Caki-1 (A) and A-10 (B) whole cell lysates and rat heart (C) and mouse heart (D) tissue extracts.

ANP (23/1) - sc-80686 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
ANP (23/1): sc-80686. Western blot analysis of ANP expression in rat heart tissue extract.

Anti-ANP [EPR15838] antibody - C-terminal - ab191398 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR15838
All lanes : Anti-ANP [EPR15838] antibody - C-terminal (ab191398) at 1/10000 dilutionLane 1 : Human fetal heart lysateLane 2 : Human skeletal muscle lysateLane 3 : PC3 cell lysateLane 4 : A673 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Anti-ANP antibody - ab40791 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab40791 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab40791 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-ANP antibody - ab76743 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC image of ab76743 staining in Human Heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab76743, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-ANP antibody - ab47698 from Abcam

Prices $370.00
Sizes 150 µg
Host Rabbit
Clonality Polyclonal

Anti-ANP antibody [M622709] - ab2093 from Abcam

Prices $372.00
Sizes 500 µg
Host Mouse
Clonality Monoclonal
Clone M622709

Anti-ANP antibody - N-terminal - ab135135 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-ANP antibody - N-terminal (ab135135) at 1/1000 dilution + Mouse heart total cell extract

Anti-Cardiodilatin antibody - ab47687 from Abcam

Prices $370.00
Sizes 150 µg
Host Rabbit
Clonality Polyclonal

Anti-Cardiodilatin antibody (Biotin) - ab47862 from Abcam

Prices $370.00
Sizes 150 µg
Host Rabbit
Clonality Polyclonal

Anti-proANP antibody - ab180649 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-proANP antibody (ab180649) at 1/500 dilution + PC3 cell line extract

Anti-proANP antibody - ab126149 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-proANP antibody (ab126149) at 1/1000 dilutionLane 1 : NT2D1 whole cell lysateLane 2 : PC-3 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-proANP antibody - ab125276 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-proANP antibody (ab125276) at 1/500 dilution + 134 aa Human BNP recombinant protein

Anti-proANP antibody - ab47692 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-proANP antibody - ab85306 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of proANP staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85306, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-proANP antibody - ab14160 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-NPPA / ANP Antibody - LS-C12038 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NPPA / ANP Antibody (aa4-28, clone 23/1) - LS-C11224 from LifeSpan Bioscience

Prices $605.00
Sizes 1 mg
Host Mouse
Clonality Monoclonal
Clone 23/1

Anti-NPPA / ANP Antibody - LS-C12037 from LifeSpan Bioscience

Prices $995.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NPPA / ANP Antibody - LS-C12039 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NPPA / ANP Antibody - LS-C12040 from LifeSpan Bioscience

Prices $995.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NPPA / ANP Antibody - LS-C35071 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NPPA / ANP Antibody - LS-C35072 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NPPA / ANP Antibody - LS-C35073 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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