CTNNB1
| Gene Symbol | CTNNB1 |
|---|---|
| Entrez Gene | 1499 |
| Alt Symbol | CTNNB, MRD19, armadillo |
| Species | Human |
| Gene Type | protein-coding |
| Description | catenin (cadherin-associated protein), beta 1, 88kDa |
| Other Description | catenin beta-1 |
| Swissprots | Q8NEW9 P35222 Q9H391 A8K1L7 Q8NI94 |
| Accessions | AAD32267 AAL89457 AAR18817 ACD11099 ACD11101 AEW16366 CCH22596 EAW64625 EAW64626 EAW64627 EAW64628 P35222 AB062292 BAB93475 AB451264 BAG70078 AB451392 BAG70206 AK095242 AK289932 BAF82621 AK294778 BAG57905 AK296790 BAG59368 AK299954 BAG61781 AK314776 BC058926 AAH58926 BX648430 DA216720 EU446854 ABZ92383 U38440 X87838 CAA61107 Z19054 CAA79497 XM_005264886 XP_005264943 XM_006712983 XP_006713046 XM_006712984 XP_006713047 XM_006712985 XP_006713048 NM_001098209 NP_001091679 NM_001098210 NP_001091680 NM_001904 NP_001895 |
| Function | Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:212 |
| Subcellular Location | Cytoplasm. Nucleus {ECO:0000269|PubMed:24342833}. Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell junction. Cell membrane {ECO:0000269|PubMed:24342833}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Note=Colocalized with RAPGEF2 and TJP1 at cell-cell contacts (By similarity). Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. The majority of beta-catenin is localized to the cell membrane. In interphase, colocalizes with CROCC between CEP250 puncta at the proximal end of centrioles, and this localization is dependent on CROCC and CEP250. In mitosis, when NEK2 activity increases, it localizes to centrosomes at spindle poles independent of CROCC. Colocalizes with CDK5 in the cell-cell |
| Tissue Specificity | Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level). {ECO:0000269|PubMed:11703283, ECO:0000269|PubMed:17009320, ECO:0000269|PubMed:17289029}. |
| Top Pathways | Proteoglycans in cancer, HTLV-I infection, Hippo signaling pathway, Signaling pathways regulating pluripotency of stem cells, Rap1 signaling pathway |
SignalSilence ® β-Catenin siRNA II - 6238 from Cell Signaling Technology
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SignalSilence ® β-Catenin siRNA I - 6225 from Cell Signaling Technology
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β-catenin siRNA (h) - sc-29209 from Santa Cruz Biotechnology
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β-catenin shRNA Plasmid (h) - sc-29209-SH from Santa Cruz Biotechnology
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β-catenin shRNA (h) Lentiviral Particles - sc-29209-V from Santa Cruz Biotechnology
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β-catenin siRNA (h2) - sc-44252 from Santa Cruz Biotechnology
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β-catenin shRNA Plasmid (h2) - sc-44252-SH from Santa Cruz Biotechnology
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β-catenin shRNA (h2) Lentiviral Particles - sc-44252-V from Santa Cruz Biotechnology
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beta-Catenin RNAi - H00001499-R01V from Novus Biologicals
![Western Blot: beta-Catenin RNAi [H00001499-R01V] - Analysis of CTNNB1 over-expressed 293 cell line, cotransfected with CTNNB1 Validated Chimera RNAi or non-transfected control. Blot probed with H00001499-M02. GAPDH (36.1 kDa) used as loading control.](http://www.bioprodhub.com/system/product_images/rna_products/5/sub_1/15691_beta-Catenin-RNAi-Western-Blot-H00001499-R01V-img0002.jpg)
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MISSION® esiRNA - EHU139421 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN1338 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND1338 from Sigma-Aldrich
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CTNNB1 siRNA (Human) - CRH1060 from Cohesion Biosciences
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beta-Catenin RNAi - H00001499-R01 from Novus Biologicals
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CTNNB1 - MBS8223926 from MyBioSource
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shRNA set against Human CTNNB1 (NM_001904.3) - SHH271309 from Creative biogene
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shRNA set against Human CTNNB1(NM_001904.3) - SHG171685 from Creative biogene
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Human CTNNB1 siRNA - orb261562 from Biorbyt
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CTNNB1 shRNA Plasmid (Human) - CHH1060 from Cohesion Biosciences
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CTNNB1 miRNA 3'UTR clone - MiUTR1H-02525 from Creative biogene
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CTNNB1 miRNA 3'UTR clone - MiUTR1H-02526 from Creative biogene
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CTNNB1 miRNA 3'UTR clone - MiUTR1H-02527 from Creative biogene
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