ANXA1

Gene Symbol ANXA1
Entrez Gene 301
Alt Symbol ANX1, LPC1
Species Human
Gene Type protein-coding
Description annexin A1
Other Description annexin I (lipocortin I)|annexin-1|calpactin II|calpactin-2|chromobindin-9|phospholipase A2 inhibitory protein
Swissprots P04083
Accessions ADZ76495 EAW62545 EAW62546 EAW62547 EAW62548 EAW62549 EAW62550 P04083 AA039737 AB451274 BAG70088 AB451401 BAG70215 AK074480 AK296808 BAG59381 AK311684 BC001275 AAH01275 BC018683 BC034157 AAH34157 BC035993 AAH35993 BT019896 AAV38699 BT019916 AAV38719 BT019917 AAV38720 CR407684 CAG28612 CX870105 DQ891173 ABM82099 DQ894338 ABM85264 X05908 CAA29338 XM_011518608 XP_011516910 XM_011518609 XP_011516911 NM_000700 NP_000691
Function Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
Subcellular Location Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}. Cell projection, cilium {ECO:0000250}. Basolateral cell membrane {ECO:0000250}. Note=Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium. Found in the cytoplasm of type II pneumocytes and alveolar macrophages. {ECO:0000250}.

Annexin A1 (D16A10) Rabbit mAb - 8691 from Cell Signaling Technology

Prices $239.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D16A10
Western blot analysis of extracts from various cell lines using Annexin A1 (D16A10) Rabbit mAb.

Annexin A1 Antibody - 3299 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using Annexin A1 Antibody.

Annexin I (EH17a) - sc-12740 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Annexin I (EH17a): sc-12740. Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic localization.

Annexin I (H-65) - sc-11387 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Annexin I (H-65): sc-11387. Western blot analysis of Annexin I expression in K-562 whole cell lysate.

Annexin I (ANEX 5E4/1) - sc-53158 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Annexin I (ANEX 5E4/1): sc-53158. Western blot analysis of Annexin I expression in SK-N-SH (A), K-562 (B), U-937 (C) and PC-3 (D) whole cell lysates.

Annexin I (ANEX 6E4/3) - sc-53159 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Annexin I (ANEX 6E4/3): sc-53159. Western blot analysis of Annexin I expression in non-transfected: sc-117752 (A) and human Annexin I transfected: sc-110462 (B) 293T whole cell lysates.

Annexin I (N-19) - sc-1923 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Annexin I (N-19): sc-1923. Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic localization.

Annexin I (39) - sc-130305 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Annexin I (39): sc-130305. Western blot analysis of human recombinant Annexin I.

Annexin I (74/3) - sc-65872 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Annexin I (74/3): sc-65872. Western blot analysis of Annexin I expression in ECV304 (A) and K-562 (B) whole cell lysates.

p-Annexin I (86.Tyr 21) - sc-135751 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

p-Annexin I (295.Tyr 21) - sc-135750 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

Anti-Annexin A1 antibody - ab33061 from Abcam

Prices $392.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-Annexin A1 antibody - ab168468 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Annexin A1 antibody (ab168468) at 1 µg/mlLane 1 : Annexin A1 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.

Anti-Annexin A1 antibody - ab88865 from Abcam

Prices $384.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-Annexin A1 antibody (ab88865) at 1 µg/ml + human spleen tissue lysate at 50 µg

Anti-Annexin A1 antibody - ab97485 from Abcam

Prices $384.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-Annexin A1 antibody (ab97485) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

Anti-Annexin A1 antibody - ab47661 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Annexin A1 antibody (ab47661) at 1 µg/mlLane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate (ab7913)Lane 2 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-Annexin A1 antibody - ab88060 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-Annexin A1 antibody (ab88060) at 5 µg/ml + immunogen (recombinant full length tagged protein) at 0.2 µgSecondaryGoat Anti-Mouse IgG at 1/5000 dilution

Anti-Annexin A1 antibody - ab134691 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Annexin A1 antibody (ab134691) at 1/500 dilution + HeLa cell extract at 30 µg

Anti-Annexin A1 antibody - ab46686 from Abcam

Prices $376.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Anti-Annexin A1 antibody (ab46686) at 1/250 dilution + THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate (ab7913) at 10 µgSecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-Annexin A1 antibody - ab41830 from Abcam

Prices $376.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
Ab41830 staining human Annexin I in human placenta by formalin fixed paraffin embedded immunohistochemistry at 1/25 dilution.

Anti-Annexin A1 antibody [2F1] - ab135256 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2F1
Anti-Annexin A1 antibody [2F1] (ab135256) at 1/500 dilution + Human Annexin A1 recombinant protein.

Anti-Annexin A1 antibody [3A8] - ab118060 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3A8
All lanes : Anti-Annexin A1 antibody [3A8] (ab118060) at 1/2000 dilutionLane 1 : HEK293T cell lysate, transfected with pCMV6-ENTRY control Lane 2 : HEK293T cell lysate, transfected with pCMV6-ENTRY Annexin A1 Lysates/proteins at 5 µg per lane.

Anti-Annexin A1 antibody [6E4/3] - ab4635 from Abcam

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 6E4/3
Ab4635 staining Human normal esophagus. Staining is localized to the nucleus.Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Annexin A1 antibody, prediluted - ab41831 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
Ab41831 staining human Annexin A1 in human placenta by immunohistochemistry using formalin fixed, paraffin embedded tissue.

Anti-Annexin A1 antibody - Carboxyterminal end - ab65844 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Annexin A1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Annexin A1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab65844.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 39kDa; Annexin A1, non specific bands - 52kDa and in control (lane 2): We are unsure as to the identity of this extra band.
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