NFX1

Gene Symbol NFX1
Entrez Gene 4799
Alt Symbol NFX2, TEG-42, Tex42
Species Human
Gene Type protein-coding
Description nuclear transcription factor, X-box binding 1
Other Description nuclear transcription factor, X box-binding protein 1|transcriptional repressor NF-X1
Swissprots Q9BXI1 Q96EL5 Q12986 Q5VXW6 A8K6H8
Accessions AAB94787 EAW58507 EAW58508 EAW58509 EAW58510 EAW58511 Q12986 AF332009 AAK16545 AI679589 AK055847 AK225750 AK291643 BAF84332 AL832342 AW674001 BC006447 AAH06447 BC012151 AAH12151 BP871782 BX647337 DA093124 DQ893676 ABM84602 DQ895843 ABM86769 U15306 AAA69517 XM_005251473 XP_005251530 XM_005251474 XP_005251531 XM_005251475 XP_005251532 NM_002504 NP_002495 NM_147133 NP_667344 NM_147134 NP_667345
Function Binds to the X-box motif of MHC class II genes and represses their expression. May play an important role in regulating the duration of an inflammatory response by limiting the period in which MHC class II molecules are induced by interferon-gamma. Isoform 3 binds to the X-box motif of TERT promoter and represses its expression. Together with PABPC1 or PABPC4, isoform 1 acts as a coactivator for TERT expression. Mediates E2-dependent ubiquitination. {ECO:0000269|PubMed:10500182, ECO:0000269|PubMed:15371341, ECO:0000269|PubMed:17267499}.
Subcellular Location Nucleus.

NFX1 (E-15) - sc-163135 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

NFX1 (Y-14) - sc-100973 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
NFX1 (Y-14): sc-100973. Western blot analysis of NFX1 expression in HeLa nuclear extract.

Anti-NFX1 antibody - ab176733 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NFX1 antibody (ab176733) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgLane 5 : NIH3T3 whole cell lysate at 50 µgdeveloped using the ECL technique

Anti-NFX1 antibody - ab86207 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-NFX1 antibody (ab86207) at 1 µg/ml (5% skim milk/ PBS buffer) + Jurkat cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-NFX1 antibody - ab83833 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-NFX1 antibody (ab83833) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-NFX1 antibody - ab58347 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-NFX1 Antibody (aa1-50) - LS-C289623 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NFX1 Antibody (aa1070-1120) - LS-C289625 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NFX1 Antibody (aa250-300) - LS-C289624 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NFX1 Antibody (aa981-1081, Azide-free) - LS-C328071 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-NFX1 Antibody (N-Terminus) - LS-C101516 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
NFX1 antibody LS-C101516 Western blot of Jurkat lysate.

Anti-NFX1 Antibody (clone 1D12) - LS-C197777 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1D12
Immunofluorescence of monoclonal antibody to NFX1 on HeLa cell. [antibody concentration 30 ug/ml]

Anti-NFX1 Antibody (C-Terminus) IHC-plus™ - LS-B3576 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-NFX1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3576 concentration 5 ug/ml.

HDAC6 Antibody - NB100-56343 from Novus Biologicals

Prices $99.00, $299.00
Sizes 25 µg, 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: HDAC6 Antibody [NB100-56343] - analysis of HDAC-6 in NIH 3T3 cell lysate with this antibody.

NFX1 Antibody (1D12) - H00004799-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1D12
Western Blot: NFX1 Antibody (1D12) [H00004799-M01] - NFX1 monoclonal antibody (M01), clone 1D12 Analysis of NFX1 expression in Hela S3 NE.

NFX1 Antibody - NBP1-49933 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: NFX1 Antibody [NBP1-49933] - Detection of Human and Mouse NFX1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-NFX1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. NFX1 was also immunoprecipitated by rabbit anti-NFX1 antibodies which recognize upstream epitopes. For blotting immunoprecipitated NFX1 was used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

NFX1 Antibody - NBP1-49932 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunoprecipitation: NFX1 Antibody [NBP1-49932] - Detection of Human NFX1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NFX1 antibody used for IP at 3 mcg/mg lysate. NFX1 was also immunoprecipitated by rabbit anti-NFX1 antibodies which recognize other epitopes. For blotting immunoprecipitated NFX1 was used at 0.4 mcg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

NFX1 Antibody - NBP1-49931 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

NFX1 Antibody - H00004799-A01 from Novus Biologicals

Host Mouse
Clonality Polyclonal

NFX1 Polyclonal Antibody - bs-8377R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Monoclonal Anti-NFX1 antibody produced in mouse - WH0004799M1 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1D12
<B>Immunoblotting</B><BR/>Monoclonal Anti-NFX1, <B>Cat. No. WH0004799M1</B> used at the antibody concentration: 1 ~ 5 μg/mL. Specific band of ~123.1 kDa using Hela NE lysate.

Anti-NFX1 (231-245) antibody produced in rabbit - SAB1100154 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-NFX1 (231-245), <B>Cat. No. SAB1100154</B>: Immunofluorescence of HUVEC cells using Anti-NFX1 (231-245), SAB1100154 (red), taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Anti-NFX1 (ab2) antibody produced in rabbit - SAB2101586 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-NFX1 (ab2): <B>Cat. No.SAB2101586</B>: NFX1 in Jurkat cell lysate with NFX1 antibody at 1.0 μg/mL.

Anti-NFX1 (ab1) antibody produced in rabbit - SAB2101585 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-NFX1 (ab1): <B>Cat. No. SAB2101585</B>: Immunohistochemistry of NFX1 in Human Testis tissue with NFX1 antibody at 4-8 μg/mL.

NFX1 antibody - N-terminal region (ARP34770_P050) - ARP34770_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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