NSFL1C

Gene Symbol NSFL1C
Entrez Gene 55968
Alt Symbol P47, UBX1, UBXD10, UBXN2C, dJ776F14.1
Species Human
Gene Type protein-coding
Description NSFL1 (p97) cofactor (p47)
Other Description NSFL1 cofactor p47|SHP1 homolog|UBX domain-containing protein 2C|p97 cofactor p47
Swissprots Q7Z533 Q5JXA5 Q9NVL9 A2A2L1 B2RD74 Q9UNZ2 Q5JXA4 Q9UI06 Q9H102
Accessions EAX10627 EAX10628 EAX10629 EAX10630 EAX10631 EAX10632 Q9UNZ2 AA948047 AF078856 AAD44488 AF086909 AAP97139 AF112211 AAF17199 AF283774 AK001511 BAA91731 AK023585 AK093845 BAG52771 AK128722 BAC87590 AK223334 BAD97054 AK223341 BAD97061 AK297403 BAG59842 AK315433 BAG37821 BC002801 AAH02801 BE795675 BQ007132 DB173022 DB202404 DQ893189 ABM84115 DQ896513 ABM87512 XM_006723592 XP_006723655 XM_006723593 XP_006723656 XM_006723594 XP_006723657 XM_011529300 XP_011527602 XM_011529301 XP_011527603 XM_011529302 XP_011527604 XM_011529303 XP_011527605 XM_011529304 XP_011527606 XM_011529305 XP_011527607 XM_011529306 XP_011527608 NM_001206736 NP_001193665 NM_016143 NP_057227 NM_018839 NP_061327 NR_038164 NM_182483
Function Reduces the ATPase activity of VCP. Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP- mediated formation of transitional endoplasmic reticulum (tER) (By similarity). Inhibits the activity of CTSL (in vitro). {ECO:0000250}.
Subcellular Location Nucleus {ECO:0000250}. Golgi apparatus, Golgi stack {ECO:0000250}. Chromosome {ECO:0000250}. Note=Predominantly nuclear in interphase cells. Bound to the axial elements of sex chromosomes in pachytene spermatocytes. A small proportion of the protein is cytoplasmic, associated with Golgi stacks (By similarity). {ECO:0000250}.
Top Pathways Protein processing in endoplasmic reticulum

p47 (D-9) - sc-365215 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p47 (D-9): sc-365215. Western blot analysis of p47 expression in non-transfected: sc-117752 (A) and human p47 transfected: sc-175045 (B) 293T whole cell lysates.

p47 (E-16) - sc-86190 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p47 (E-16): sc-86190. Western blot analysis of p47 expression in 293T whole cell lysate.

p47 (E-7) - sc-376614 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p47 (E-7): sc-376614. Western blot analysis of p47 expression in non-transfected: sc-117752 (A) and human p47 transfected: sc-175045 (B) 293T whole cell lysates.

p47 (G-16) - sc-86191 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p47 (G-16): sc-86191. Western blot analysis of p47 expression in non-transfected: sc-117752 (A) and human p47 transfected: sc-175045 (B) 293T whole cell lysates.

p47 (H-85) - sc-134945 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
p47 (H-85): sc-134945. Western blot analysis of p47 expression in non-transfected: sc-117752 (A) and human p47 transfected: sc-175045 (B) 293T whole cell lysates.

Anti-NSFL1C antibody - ab88685 from Abcam

Host Mouse
Clonality Polyclonal
Anti-NSFL1C antibody (ab88685) at 1 µg/ml + Human placenta tissue lysate at 50 µg

Anti-NSFL1C Antibody - LS-C334867 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using NSFL1C antibody.

Anti-NSFL1C Antibody (aa21-302) - LS-C185679 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded differential N/T pair Lung SCC cancer xenograft using NSFL1C antibody at 1:100 dilution.

NSFL1C Antibody - H00055968-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: NSFL1C Antibody [H00055968-B01P] - Analysis of NSFL1C expression in human placenta.

NSFL1C Antibody - NBP1-31402 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

NSFL1C Antibody - NBP2-36529 from Novus Biologicals

Prices $349.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: NSFL1C Antibody [NBP2-36529] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NSFL1C antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

NSFL1C Antibody - NBP2-13677 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

NSFL1C Antibody - NBP2-13676 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: NSFL1C Antibody [NBP2-13676] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp

NSFL1C Antibody - H00055968-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-NSFL1C antibody produced in rabbit - HPA047108 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-NSFL1C:<B>Cat. No. HPA047108</B>: Immunohistochemical staining of human testis shows distinct nuclear and cytoplasmic positivity in cells in seminiferus ducts.

Anti-NSFL1C antibody produced in mouse - SAB1400638 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Polyclonal Anti-NSFL1C, antibody produced in mouse, purified immunoglobulin: <B>Cat. No. SAB1400638</B>: Antibody concentration: 1.0 ~ 5.0 μg/mL. Specific band of ~40.6 kDa using human placenta lysate.

Anti-NSFL1C (136-150) antibody produced in rabbit - SAB1101878 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human NSFL1C was separated on SDS-PAGE, blotted with Anti- NSFL1C (136-150) (<B>Cat. No. SAB1101878</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500 <BR/>2. Antibody dilution 1:500 + NSFL1C immunizing peptide (human, 136-150)

Anti-NSFL1C antibody produced in rabbit - HPA050628 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-NSFL1C:<B>Cat. No. HPA050628</B>: Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferus ducts and Leydig cells.

NSFL1C antibody [N1C2] - GTX105035 from GeneTex

Prices $119.00
Sizes 25 μl
Host Rabbit
Clonality Polyclonal
Anti-NSFL1C antibody [N1C2] used in Western blot. GTX105035

NSFL1 cofactor p47 antibody - orb69677 from Biorbyt

Prices $723.00
Sizes 100 μl
Host Rabbit
Clonality Polyclonal
Western blot analysis of whole cell lysate using NSFL1 cofactor p47 antibody

NSFL1C antibody - 70R-12870 from Fitzgerald

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

NSFL1C Polyclonal Antibody - A6677 from ABclonal

Prices $130.00, $220.00, $380.00
Sizes 50 µl, 100 µl, 200 µl
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using NSFL1C antibody.

NSFL1C antibody (OAGA00373) - OAGA00373 from Aviva Systems Biology

Prices $340.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

NSFL1C Antibody - abx005119 from Abbexa

Host Rabbit
Clonality Polyclonal

NSFL1C antibody - 15620-1-AP from Acris Antibodies

Prices $270.00
Sizes 150 µl
Host Rabbit
Clonality Polyclonal
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15620-1-AP(NSFL1C antibody) at dilution of 1:800
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