POLQ
| Gene Symbol | POLQ |
|---|---|
| Entrez Gene | 10721 |
| Alt Symbol | POLH, PRO0327 |
| Species | Human |
| Gene Type | protein-coding |
| Description | polymerase (DNA directed), theta |
| Other Description | DNA polymerase eta|DNA polymerase theta |
| Swissprots | Q6VMB5 O75417 O95160 |
| Accessions | EAW79510 EAW79511 EAW79512 EAW79513 EAW79514 O75417 AA767021 AB209867 BAD93104 AF043628 AAD05272 AF052573 AAC33565 AF090919 AAF24036 AW021928 AY032677 AAK39635 AY338826 AAR08421 BC034254 BC172289 AAI72289 BP365212 CR936627 CAI56770 XM_011512343 XP_011510645 XM_011512344 XP_011510646 XM_011512345 XP_011510647 XM_011512346 XP_011510648 XM_011512347 XP_011510649 XM_011512348 XP_011510650 XM_011512349 XP_011510651 XM_011512350 XP_011510652 XM_011512351 XP_011510653 XM_011512352 XP_011510654 XM_011512353 XP_011510655 XM_011512354 XP_011510656 XR_924091 XR_924092 XR_924093 NM_199420 NP_955452 |
| Function | DNA polymerase that promotes microhomology-mediated end- joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery triggered in response to double-strand breaks in DNA (PubMed:25642963, PubMed:25643323). MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation (PubMed:25642963, PubMed:25643323). POLQ acts as an inhibitor of homology-recombination repair (HR) pathway by limiting RAD51 accumulation at resected ends (PubMed:25642963). POLQ-mediated MMEJ may be required to promote the survival of cells with a compromised HR repair pathway, thereby preventing genomic havoc by resolving unrepaired lesions (By similarity). The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs. It then extends each strand from the base- |
| Subcellular Location | Nucleus {ECO:0000250|UniProtKB:O18475}. Chromosome {ECO:0000269|PubMed:25642963}. Note=Enriched in chromatin in response to ultaviolet (UV) light (PubMed:25642963). Binds to chromatin during early G1 (PubMed:24989122). {ECO:0000269|PubMed:24989122, ECO:0000269|PubMed:25642963}. |
| Tissue Specificity | Highly expressed in testis. {ECO:0000269|PubMed:14576298}. |
DNA pol θ CRISPR/Cas9 KO Plasmid (h) - sc-407414 from Santa Cruz Biotechnology
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DNA pol θ HDR Plasmid (h) - sc-407414-HDR from Santa Cruz Biotechnology
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DNA pol θ Double Nickase Plasmid (h) - sc-407414-NIC from Santa Cruz Biotechnology
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DNA pol θ Double Nickase Plasmid (h2) - sc-407414-NIC-2 from Santa Cruz Biotechnology
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DNA pol θ CRISPR Activation Plasmid (h) - sc-407414-ACT from Santa Cruz Biotechnology
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DNA pol θ CRISPR Activation Plasmid (h2) - sc-407414-ACT-2 from Santa Cruz Biotechnology
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DNA pol θ Lentiviral Activation Particles (h) - sc-407414-LAC from Santa Cruz Biotechnology
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DNA pol θ Lentiviral Activation Particles (h2) - sc-407414-LAC-2 from Santa Cruz Biotechnology
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DNA pol θ siRNA (h) - sc-37789 from Santa Cruz Biotechnology
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DNA pol θ shRNA Plasmid (h) - sc-37789-SH from Santa Cruz Biotechnology
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DNA pol θ shRNA (h) Lentiviral Particles - sc-37789-V from Santa Cruz Biotechnology
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XPV/DNA polymerase eta RNAi - H00005429-R01 from Novus Biologicals
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MISSION® esiRNA - EHU041581 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN17105 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND17105 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR07246 from Sigma-Aldrich
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POLQ siRNA (Human) - CRH7304 from Cohesion Biosciences
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shRNA set against Human POLQ (NM_199420.3) - SHH379308 from Creative biogene
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POLQ shRNA Plasmid (Human) - CHH7304 from Cohesion Biosciences
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POLQ miRNA 3'UTR clone - MiUTR1H-08069 from Creative biogene
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