SMYD1

Gene Symbol	SMYD1
Entrez Gene	150572
Alt Symbol	BOP, KMT3D, ZMYND18, ZMYND22
Species	Human
Gene Type	protein-coding
Description	SET and MYND domain containing 1
Other Description	CD8 beta opposite\|SET and MYND domain-containing protein 1\|histone-lysine N-methyltransferase SMYD1\|zinc finger, MYND domain containing 18
Swissprots	A0AV30 Q8NB12 A6NE13
Accessions	EAW77077 EAW77078 EAW77079 EAW77080 Q8NB12 AF086123 AK022290 AK091724 BAC03732 AL832035 CAI46139 AY518933 AAT44535 AY518934 AAT44536 BC017915 BC126191 AAI26192 BX647878 CAI46077 EU831908 ACE87349 EU832002 ACE86665 HQ258264 ADR83018 XM_005264156 XP_005264213 NM_198274 NP_938015
Function	Methylates histone H3 at 'Lys-4' (H3K4me), seems able to perform both mono-, di-, and trimethylation. Acts as a transcriptional repressor. Essential for cardiomyocyte differentiation and cardiac morphogenesis.
Subcellular Location	Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
Tissue Specificity	Expression seems mostly restricted to heart and skeletal muscle. {ECO:0000269\|PubMed:19783823}.

SMYD1 (C-19) - sc-79080 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

SMYD1 (C-19): sc-79080. Western blot analysis of SMYD1 expression in non-transfected: sc-117752 (A) and human SMYD1 transfected: sc-177956 (B) 293T whole cell lysates.

SMYD1 (H-300) - sc-366895 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

SMYD1 (H-300): sc-366895. Western blot analysis of SMYD1 expression in human heart tissue extract.

Anti-SMYD1 antibody - ab127570 from Abcam

Prices	$384.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-SMYD1 antibody (ab127570) at 1/10000 dilutionLane 1 : 293T whole cell lysateLane 2 : A431 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-SMYD1 antibody - ab183878 from Abcam

Prices	$376.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-SMYD1 antibody (ab183878) at 1/1000 dilution + mouse heart whole cell lysate at 50 µg

Anti-SMYD1 antibody [EPR13573] - ab185969 from Abcam

Prices	$376.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR13573

All lanes : Anti-SMYD1 antibody [EPR13573] (ab185969) at 1/1000 dilutionLane 1 : Mouse heart lysateLane 2 : Rat heart lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique

Anti-SMYD1 antibody [EPR13574(B)-30] - ab181372 from Abcam

Prices	$384.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR13574(B)-30

All lanes : Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372) at 1/1000 dilutionLane 1 : Human fetal heart lysatesLane 2 : Human skeletal muscle lysatesLysates/proteins at 10 µg per lane.

Anti-SMYD1 antibody [none] - ab150718 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	none

Anti-SMYD1 antibody [none] (ab150718) at 1/2000 dilution + rat skeletal muscle lysates

Anti-SMYD1 antibody - N-terminal - ab176370 from Abcam

Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-SMYD1 antibody - N-terminal (ab176370) at 1/1000 dilutionLane 1 : HeLa total cell extractLane 2 : Jurkat total cell extract

Anti-SMYD1 Antibody - LS-C346351 from LifeSpan Bioscience

Prices	$255.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Immunofluorescence blot of MCF7 cell using SMYD1 antibody. Blue: DAPI for nuclear staining.

Anti-SMYD1 Antibody (aa420-449) - LS-C161331 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

SMYD1 Antibody western blot of HeLa cell line lysates (35 ug/lane). The SMYD1 antibody detected the SMYD1 protein (arrow).

Anti-SMYD1 Antibody (N-Terminus) - LS-C30100 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-SMYD1 Antibody (aa420-449, APC) - LS-C241038 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-SMYD1 Antibody (aa420-449, AP) - LS-C241037 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-SMYD1 Antibody (aa107-156) - LS-C107796 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

SMYD1 antibody LS-C107796 Western blot of ACHN lysate.

Anti-SMYD1 Antibody (aa420-449, Biotin) - LS-C241039 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-SMYD1 Antibody (aa420-449, PE) - LS-C241042 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-SMYD1 Antibody (aa420-449, FITC) - LS-C241041 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-SMYD1 Antibody (aa420-449, Azide-free, HRP) - LS-C248208 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-SMYD1 Antibody (aa420-449) - LS-C325219 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-SMYD1 Antibody - LS-C342273 from LifeSpan Bioscience

Prices	$385.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

Anti-SMYD1 Antibody (aa395-444) - LS-C107797 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

SMYD1 antibody LS-C107797 Western blot of HeLa lysate.

SmyD1 Antibody - NBP2-20428 from Novus Biologicals

Prices	$329.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: SmyD1 Antibody [NBP2-20428] - Sample (50 ug of whole cell lysate) A: Mouse Heart, 7. 5% SDS PAGE gel, diluted at 1:10000.

SmyD1 Antibody - NBP2-38957 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: SmyD1 Antibody [NBP2-38957] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Skeletal muscle tissue

Anti-SMYD1 antibody produced in rabbit - HPA040790 from Sigma-Aldrich

Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-SMYD1: <B>Cat. No. HPA040790</B>: Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.

Anti-SMYD1 antibody produced in rabbit - SAB2104994 from Sigma-Aldrich

Prices	$347.50
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-SMYD1, antibody produced in rabbit: <B>Cat. No. SAB2104994</B>: Immunoblotting analysis of SMYD1 in HeLa cell lysate with SMYD1 anitbody at 1 μg/mL.

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