MED25

Gene Symbol MED25
Entrez Gene 81857
Alt Symbol ACID1, ARC92, CMT2B2, P78, PTOV2, TCBAP0758
Species Human
Gene Type protein-coding
Description mediator complex subunit 25
Other Description ARC/mediator transcriptional coactivator subunit|activator interaction domain-containing protein 1|activator-recruited cofactor 92 kDa component|mediator of RNA polymerase II transcription subunit 25|mediator of RNA polymerase II transcription, subunit 25 homolog|prostate-derived protein 78 kDa
Swissprots Q9HB34 Q707U4 O95783 Q71SY5 Q8TB55 B9TX30 Q6P143 Q9H0L5 Q6QMH5 A8K095
Accessions AAD15565 EAW52544 EAW52545 EAW52546 EAW52547 EAW52548 EAW52549 EAW52550 EAW52551 Q71SY5 AF261072 AAM20739 AF283769 AAG15589 AF447873 AAQ04648 AJ617479 CAE84581 AK055844 BAG51583 AK289460 BAF82149 AK298897 BAG61008 AK307330 AL136746 CAB66680 AY533507 AAS45401 BC021543 BC024312 AAH24312 BC065297 AAH65297 BG770032 BI520216 DC410997 EU392499 ACB88861 EU392500 ACB88862 EU392501 ACB88863 EU392502 ACB88864 EU392503 ACB88865 EU392504 ACB88866 EU392505 ACB88867 EU392506 ACB88868 EU392507 ACB88869 EU392508 ACB88870 EU392509 ACB88871 EU392510 ACB88872 EU392511 ACB88873 EU392512 ACB88874 EU392513 ACB88875 EU392514 ACB88876 EU392515 ACB88877 EU392516 ACB88878 EU392517 ACB88879 EU392518 ACB88880 EU392519 EU832505 ACE87677 EU832586 ACE86988 XM_011527353 XP_011525655 NM_030973 NP_112235
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription. {ECO:0000269|PubMed:14657022, ECO:0000269|PubMed:14983011, ECO:0000269|PubMed:17641689}.
Subcellular Location Nucleus {ECO:0000269|PubMed:14657022}.
Tissue Specificity Ubiquitously expressed. Highest levels in brain, heart, kidney, peripheral leukocytes, placenta, skeletal muscle and spleen. {ECO:0000269|PubMed:14657022, ECO:0000269|PubMed:14983011}.

Med25 (A-7) - sc-393759 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Med25 (A-7): sc-393759. Western blot analysis of Med25 expression in IMR-32 nuclear extract (A) and MCF7 whole cell lysate (B).

Med25 (E-13) - sc-161111 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Med25 (H-194) - sc-366990 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Med25 (N-15) - sc-161112 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-MED25 antibody - ab107551 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-MED25 antibody (ab107551) at 1/100 dilution + Mouse kidney tissue lysates at 35 µg

Anti-MED25 antibody - C-terminal - ab187170 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MED25 antibody - C-terminal (ab187170) at 1/1000 dilutionLane 1 : Mouse brain total cell extractLane 2 : HeLa total cell extractLane 3 : Jurkat total cell extract

Anti-MED25 Antibody (aa112-140, APC) - LS-C237519 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MED25 Antibody (aa112-140, AP) - LS-C237520 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MED25 Antibody (aa112-140, PE) - LS-C237523 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MED25 Antibody (aa112-140, FITC) - LS-C237521 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MED25 Antibody (aa112-140, Azide-free, HRP) - LS-C248792 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MED25 Antibody (aa112-140, Biotin) - LS-C237522 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MED25 Antibody (aa33-82) - LS-C117068 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
MED25 antibody LS-C117068 Western blot of Fetal Heart lysate.

Anti-MED25 Antibody (C-Terminus) - LS-C201584 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of Human OVCAR-3. MED25 antibody dilution 1.0 ug/ml.

Anti-MED25 Antibody (aa119-148) - LS-C322940 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MED25 Antibody (aa112-140) - LS-C164119 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
MED25 antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-MED25 antibody produced in rabbit - SAB2106906 from Sigma-Aldrich

Prices $341.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-MED25: <B>Cat. No. SAB2106906</B>: Western blot analysis of MED25 with MED25 (mediator complex subunit 25) antibody against the N terminal of MED25 (50 μg) antibody at 1.0 μg/mL.

ANTI-MED25 (N-TERM) antibody produced in rabbit - SAB1301462 from Sigma-Aldrich

Prices $326.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-MED25 (N-term): <B>Cat. No. SAB1301462 </B>: MED25 Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35 μg/lane).

MED25 antibody - N-terminal region (ARP50698_P050) - ARP50698_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

MED25 Antibody - C-terminal region (ARP50699_P050) - ARP50699_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

MED25 Antibody - N-terminal region (OAAB00209) - OAAB00209 from Aviva Systems Biology

Prices $289.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal

MED25-Antibody-N-term - AP10203a from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
MED25 Antibody (N-term) (Cat. #AP10203a) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the MED25 antibody detected the MED25 protein (arrow).

MED25 antibody - GTX53612 from GeneTex

Prices $319.00
Sizes 400 μl
Host Rabbit
Clonality Polyclonal
Anti-MED25 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX53612

MED25 antibody, N-term - GTX49103 from GeneTex

Prices $329.00
Sizes 50 μg
Host Rabbit
Clonality Polyclonal
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