DDX20

Gene Symbol DDX20
Entrez Gene 11218
Alt Symbol DP103, GEMIN3
Species Human
Gene Type protein-coding
Description DEAD (Asp-Glu-Ala-Asp) box polypeptide 20
Other Description DEAD box protein 20|DEAD box protein DP 103|DEAD-box protein DP103|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 20, 103kD|SMN-interacting protein|component of gems 3|gemin-3|probable ATP-dependent RNA helicase DDX20
Swissprots Q9UIY0 Q9NVM3 Q9Y659 Q9UHI6 Q9UF59 Q96F72 B4DWV7
Accessions EAW56507 EAW56508 Q9UHI6 AF106019 AAD42744 AF155803 AAK56799 AF171063 AAF14544 AK001506 BAA91727 AK301697 BAG63169 AK314312 AL133598 CAB63734 AY007146 AAG02003 BC011556 AAH11556 BC031062 AAH31062 BC034953 AAH34953 DB072947 DQ892249 ABM83175 DQ895449 ABM86375 NM_007204 NP_009135
Function The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs). {ECO:0000269|PubMed:18984161}.
Subcellular Location Cytoplasm. Nucleus, gem. Note=Localized in subnuclear structures next to coiled bodies, called Gemini of Cajal bodies (Gems).
Tissue Specificity Ubiquitous.
Top Pathways RNA transport

Gemin3 (C-5) - sc-374373 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Gemin3 (C-5): sc-374373. Western blot analysis of Gemin3 expression in Jurkat whole cell lysate.

Gemin3 (12H12) - sc-57007 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Gemin3 (12H12): sc-57007. Western blot analysis of Gemin3 expression in Jurkat (A), HeLa (B) and NTERA-2 cl.D1 (C) whole cell lysates and CCRF-CEM (D) and HeLa (E) nuclear extracts.

Gemin3 (D-5) - sc-271853 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Gemin3 (D-5): sc-271853. Western blot analysis of Gemin3 expression in Jurkat whole cell lysate.

Gemin3 (H-145) - sc-50405 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Gemin3 (H-145): sc-50405. Western blot analysis of Gemin3 expression in HeLa (A), Jurkat (B) and CCRF-CEM (C) nuclear extracts and Jurkat whole cell lysate (D).

Gemin3 (N-20) - sc-21430 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Gemin3 (N-20): sc-21430. Western blot analysis of Gemin3 expression in Jurkat whole cell lysate.

Gemin3 (2) - sc-135919 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/500 µl
Host Mouse
Clonality Monoclonal
Gemin3 (2): sc-135919. Western blot analysis of Gemin3 expression in Jurkat whole cell lysate.

Anti-Gemin 3 antibody - ab189850 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Gemin 3 antibody (ab189850) at 1/500 dilutionLane 1 : MCF7 cell extractLane 2 : ES-2 cell extractLane 3 : 293T cell extractLane 4 : HepG2 cell extractLane 5 : HeLa cell extractLane 6 : Mouse spleen extractLane 7 : Rat testis extractLane 8 : Mouse ovary extract

Anti-Gemin 3 antibody - ab70896 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Gemin 3 with ab70896 at 1/200 (1µg/ml). Detection: DAB.

Anti-Gemin 3 antibody - ab168512 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Anti-Gemin 3 antibody (ab168512) at 1/500 dilution + Human placenta tissue lysate at 50 µg

Anti-Gemin 3 antibody - ab88863 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-Gemin 3 antibody (ab88863) at 1 µg/ml + Human placenta tissue lysate at 50 µg

Anti-Gemin 3 antibody - ab126196 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-Gemin 3 antibody (ab126196) at 1/500 dilution + HeLa whole cell lysate at 30 µg

Anti-Gemin 3 antibody - ab123557 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-Gemin 3 antibody (ab123557) at 1/100 dilution + 293 cell lysate at 35 µg

Anti-Gemin 3 antibody - ab136107 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Gemin 3 antibody (ab136107) at 1/500 dilution + K562 cell lysate at 30 µg

Anti-Gemin 3 antibody [12H12] - ab10305 from Abcam

Prices $380.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 12H12
Overlay histogram showing HeLa cells stained with ab10305 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10305, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Gemin 3 antibody [EPR11283] - ab166913 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR11283
All lanes : Anti-Gemin 3 antibody [EPR11283] (ab166913) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLysates/proteins at 10 µg per lane.

Anti-DDX20 / GEMIN3 Antibody (aa600-650) - LS-C64593 from LifeSpan Bioscience

Prices $545.00
Sizes 20 µg
Host Rabbit
Clonality Polyclonal

Anti-DDX20 / GEMIN3 Antibody (aa675-725) - LS-C64594 from LifeSpan Bioscience

Prices $545.00
Sizes 20 µg
Host Rabbit
Clonality Polyclonal

Anti-DDX20 / GEMIN3 Antibody (C-Terminus, clone 12H12) - LS-C82218 from LifeSpan Bioscience

Prices $450.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 12H12

Anti-DDX20 / GEMIN3 Antibody (C-Terminus, clone 12H12) - LS-C82260 from LifeSpan Bioscience

Prices $380.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 12H12

Anti-DDX20 / GEMIN3 Antibody (aa713-741, FITC) - LS-C222045 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DDX20 / GEMIN3 Antibody (aa713-741, Azide-free, HRP) - LS-C222047 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DDX20 / GEMIN3 Antibody (aa713-741, PE) - LS-C222048 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DDX20 / GEMIN3 Antibody (aa713-741, AP) - LS-C226722 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DDX20 / GEMIN3 Antibody (aa651-700) - LS-C170128 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
DDX20 / GEMIN3 antibody Western Blot of Rat Kidney. Antibody dilution: 1 ug/ml.

Anti-DDX20 / GEMIN3 Antibody (aa713-741, Biotin) - LS-C226723 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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