| Gene Symbol | DDX20 |
|---|---|
| Entrez Gene | 11218 |
| Alt Symbol | DP103, GEMIN3 |
| Species | Human |
| Gene Type | protein-coding |
| Description | DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 |
| Other Description | DEAD box protein 20|DEAD box protein DP 103|DEAD-box protein DP103|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 20, 103kD|SMN-interacting protein|component of gems 3|gemin-3|probable ATP-dependent RNA helicase DDX20 |
| Swissprots | Q9UIY0 Q9NVM3 Q9Y659 Q9UHI6 Q9UF59 Q96F72 B4DWV7 |
| Accessions | EAW56507 EAW56508 Q9UHI6 AF106019 AAD42744 AF155803 AAK56799 AF171063 AAF14544 AK001506 BAA91727 AK301697 BAG63169 AK314312 AL133598 CAB63734 AY007146 AAG02003 BC011556 AAH11556 BC031062 AAH31062 BC034953 AAH34953 DB072947 DQ892249 ABM83175 DQ895449 ABM86375 NM_007204 NP_009135 |
| Function | The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs). {ECO:0000269|PubMed:18984161}. |
| Subcellular Location | Cytoplasm. Nucleus, gem. Note=Localized in subnuclear structures next to coiled bodies, called Gemini of Cajal bodies (Gems). |
| Tissue Specificity | Ubiquitous. |
| Top Pathways | RNA transport |
Gemin3 CRISPR/Cas9 KO Plasmid (h) - sc-403271 from Santa Cruz Biotechnology
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Gemin3 HDR Plasmid (h) - sc-403271-HDR from Santa Cruz Biotechnology
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Gemin3 Double Nickase Plasmid (h) - sc-403271-NIC from Santa Cruz Biotechnology
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Gemin3 Double Nickase Plasmid (h2) - sc-403271-NIC-2 from Santa Cruz Biotechnology
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Gemin3 CRISPR Activation Plasmid (h) - sc-403271-ACT from Santa Cruz Biotechnology
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Gemin3 CRISPR Activation Plasmid (h2) - sc-403271-ACT-2 from Santa Cruz Biotechnology
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Gemin3 Lentiviral Activation Particles (h) - sc-403271-LAC from Santa Cruz Biotechnology
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Gemin3 Lentiviral Activation Particles (h2) - sc-403271-LAC-2 from Santa Cruz Biotechnology
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Gemin3 siRNA (h) - sc-43798 from Santa Cruz Biotechnology
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Gemin3 shRNA Plasmid (h) - sc-43798-SH from Santa Cruz Biotechnology
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Gemin3 shRNA (h) Lentiviral Particles - sc-43798-V from Santa Cruz Biotechnology
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Gemin 3 RNAi - H00011218-R02 from Novus Biologicals
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MISSION® esiRNA - EHU000701 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN7044 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND7044 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR05867 from Sigma-Aldrich
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DDX20 siRNA (Human) - CRH7687 from Cohesion Biosciences
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DDX20 - MBS8205789 from MyBioSource
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Gemin3 CRISPR/Cas9 KO Plasmid (h2) - sc-403271-KO-2 from Santa Cruz Biotechnology
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Gemin3 HDR Plasmid (h2) - sc-403271-HDR-2 from Santa Cruz Biotechnology
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shRNA set against Human DDX20 (NM_007204.4) - SHH275865 from Creative biogene
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Human DDX20 siRNA - orb268189 from Biorbyt
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DDX20 shRNA Plasmid (Human) - CHH7687 from Cohesion Biosciences
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DDX20 miRNA 3'UTR clone - MiUTR3H-08270 from Creative biogene
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