AKAP12

Gene Symbol AKAP12
Entrez Gene 9590
Alt Symbol AKAP250, SSeCKS
Species Human
Gene Type protein-coding
Description A kinase (PRKA) anchor protein 12
Other Description A-kinase anchor protein 12|A-kinase anchor protein, 250kDa|AKAP 250|Src-Suppressed C Kinase Substrate|kinase scaffold protein gravin|myasthenia gravis autoantigen gravin
Swissprots Q68D82 Q5TGN1 Q4LE68 O00310 Q99970 Q5SZ80 Q02952 O00498
Accessions EAW47753 EAW47754 EAW47755 Q02952 AB003476 BAA19927 AB210003 BAE06085 AF001504 AAB58938 AF086250 AK024598 AK307578 AK307673 AK307739 BC000188 BC015830 BC022814 BC033829 BC046095 BC047728 AAH47728 BC070151 AAH70151 BC117679 BC117680 BC156286 AAI56287 BC157067 AAI57068 CN480611 CR749527 CAH18338 CR936604 CAI56750 DA055364 DQ584338 M96322 AAA35931 U81607 AAC51366 XM_005267233 XP_005267290 XM_005267235 XP_005267292 NM_005100 NP_005091 NM_144497 NP_653080
Function Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).
Subcellular Location Cytoplasm, cell cortex {ECO:0000305}. Cytoplasm, cytoskeleton {ECO:0000305}. Note=May be part of the cortical cytoskeleton.
Tissue Specificity Expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells.

AKAP 12 (C-12) - sc-376740 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
AKAP 12 (C-12): sc-376740. Western blot analysis of AKAP 12 expression in HCT 116 whole cell lysate.

AKAP 12 (C-19) - sc-9805 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
AKAP 12 (C-19): sc-9805. Western blot analysis of AKAP 12 expression in SK-N-SH whole cell lysate.

AKAP 12 (E-19) - sc-9806 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
AKAP 12 (E-19): sc-9806. Immunoperoxidase staining of formalin fixed, paraffin-embedded human lower stomach tissue showing cytoplasmic staining of glandular cells.

AKAP 12 (H-300) - sc-33577 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
AKAP 12 (H-300): sc-33577. Western blot analysis of AKAP 12 expression in U-251 MG whole cell lysate.

AKAP 12 (K-16) - sc-48117 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
AKAP 12 (K-16): sc-48117. Immunoperoxidase staining of formalin fixed, paraffin-embedded human lower stomach tissue showing cytoplasmic staining of glandular cells.

AKAP 12 (N-15) - sc-48119 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
AKAP 12 (N-15): sc-48119. Immunoperoxidase staining of formalin fixed, paraffin-embedded human lower stomach tissue showing cytoplasmic staining of glandular cells.

AKAP 12 (C-16) - sc-48116 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
AKAP 12 (C-16): sc-48116. Immunoperoxidase staining of formalin fixed, paraffin-embedded human lower stomach tissue showing cytoplasmic staining of glandular cells.

AKAP 12 (M-16) - sc-48118 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-AKAP12 antibody - ab9698 from Abcam

Prices $378.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
All lanes : Anti-AKAP12 antibody (ab9698) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate (ab3957)Lysates/proteins at 20 µg per lane.SecondaryRabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilutionPerformed under reducing conditions.

Anti-AKAP12 antibody [JP74] - ab49849 from Abcam

Prices $378.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone JP74

Anti-AKAP12 antibody [mAbcam87067] (Alexa Fluor® 647) - ab196719 from Abcam

Host Mouse
Clonality Monoclonal
Clone mAbcam87067
ab196719 staining AKAP12 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196719 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-AKAP12 antibody - C-terminal - ab198895 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling AKAP12 with ab198895 at 1/40 dilution.

Anti-Gravin / AKAP12 Antibody (clone 3C3) - LS-C139457 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3C3
Detection limit for recombinant GST tagged AKAP12 is approximately 3 ng/ml as a capture antibody.

Anti-Gravin / AKAP12 Antibody (aa1719-1782) IHC-plus™ - LS-B10829 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Gravin / AKAP12 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10829 concentration 7.5 ug/ml.

Anti-Gravin / AKAP12 Antibody (aa1645-1658) - LS-C139806 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-Gravin / AKAP12 Antibody (aa1650-1700) - LS-C288196 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-Gravin / AKAP12 Antibody (aa1731-1781) - LS-C288197 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-Gravin / AKAP12 Antibody - LS-C26558 from LifeSpan Bioscience

Prices $330.00
Sizes 250 µg
Host Sheep
Clonality Polyclonal
Western blotting total cellular protein from cultured rat aortic smooth muscle cells was prepared and analyze. The protein was transferred to a PVDF membrane, blocked 1XTBS, 0.1% tween 20/ 5%NFDM, probed with 5 g/ml of pre-immune serum 5 ug of S125P antibody (a 3 second exposure is shown). The blot was then washed and probed with monoclonal anti-sheep IgG coupled to HRP 1:10,000, and detected with luminol.

Anti-Gravin / AKAP12 Antibody (aa301-350) - LS-C198606 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded human brain tissue, using AKAP12 Antibody. The sample on the right was incubated with synthetic peptide.

AKAP12 Antibody - NBP1-89168 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: AKAP12 Antibody [NBP1-89168] - Staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.

AKAP12 Antibody - NBP1-31482 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

AKAP12 Antibody - NB100-68211 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

AKAP12 Antibody - NBP2-38786 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

AKAP12 Antibody - 35350002 from Novus Biologicals

Prices $299.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ELISA: AKAP12 Antibody [35350002]

AKAP12 Antibody - NB100-788 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: AKAP12 Antibody [NB100-788] - NB100-788 (0.03ug/ml) staining of HeLa cell lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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