UPF3B
| Gene Symbol | UPF3B |
|---|---|
| Entrez Gene | 65109 |
| Alt Symbol | HUPF3B, MRX62, MRXS14, RENT3B, UPF3X |
| Species | Human |
| Gene Type | protein-coding |
| Description | UPF3 regulator of nonsense transcripts homolog B (yeast) |
| Other Description | hUpf3p-X|mental retardation, X-linked 62|nonsense mRNA reducing factor 3B|regulator of nonsense transcripts 3B|up-frameshift suppressor 3 homolog B|up-frameshift suppressor 3 homolog on chromosome X |
| Swissprots | Q9H1J0 Q0VAK8 D3DWI3 Q9BZI7 D3DWI4 |
| Accessions | AFL55650 EAW89842 EAW89843 EAW89844 EAW89845 EAW89846 Q9BZI7 AF318576 AAG60691 AK093728 BAG52755 AY013251 AAG48511 BC008494 BC121017 AAI21018 BC121018 AAI21019 BF223724 BI549935 CN412717 DA949746 EG328250 XM_005262458 XP_005262515 XM_006724780 XP_006724843 XM_006724781 XP_006724844 XM_011531378 XP_011529680 XM_011531379 XP_011529681 NM_023010 NP_075386 NM_080632 NP_542199 |
| Function | Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2- UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF2 stimulates both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA upstream of exon-exon junctions. In vitro, stimulates translation; the function is independent of association with UPF2 and components of the EJC core. {ECO:0000269|PubMed:11163187, ECO:0000269|PubMed:12718880, ECO:0000269|PubMed:16209946, ECO:0000269|PubMed:16601204, ECO:0000269|PubMed:18066079}. |
| Subcellular Location | Nucleus. Cytoplasm. Note=Shuttling between the nucleus and the cytoplasm. |
| Tissue Specificity | Expressed in testis, uterus, prostate, heart, muscle, brain, spinal cord and placenta. {ECO:0000269|PubMed:11113196}. |
| Top Pathways | RNA transport, mRNA surveillance pathway |
Rent3b CRISPR/Cas9 KO Plasmid (h) - sc-406904 from Santa Cruz Biotechnology
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Rent3b HDR Plasmid (h) - sc-406904-HDR from Santa Cruz Biotechnology
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Rent3b Double Nickase Plasmid (h) - sc-406904-NIC from Santa Cruz Biotechnology
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Rent3b Double Nickase Plasmid (h2) - sc-406904-NIC-2 from Santa Cruz Biotechnology
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Rent3b CRISPR Activation Plasmid (h) - sc-406904-ACT from Santa Cruz Biotechnology
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Rent3b CRISPR Activation Plasmid (h2) - sc-406904-ACT-2 from Santa Cruz Biotechnology
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Rent3b Lentiviral Activation Particles (h) - sc-406904-LAC from Santa Cruz Biotechnology
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Rent3b Lentiviral Activation Particles (h2) - sc-406904-LAC-2 from Santa Cruz Biotechnology
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Rent3b siRNA (h) - sc-38228 from Santa Cruz Biotechnology
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Rent3b shRNA Plasmid (h) - sc-38228-SH from Santa Cruz Biotechnology
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Rent3b shRNA (h) Lentiviral Particles - sc-38228-V from Santa Cruz Biotechnology
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UPF3B RNAi - H00065109-R01 from Novus Biologicals
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UPF3B RNAi - H00065109-R02 from Novus Biologicals
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MISSION® esiRNA - EHU140591 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN22519 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND22519 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR07200 from Sigma-Aldrich
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UPF3B siRNA (Human) - CRJ2182 from Cohesion Biosciences
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UPF3B - MBS8200815 from MyBioSource
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Rent3b CRISPR/Cas9 KO Plasmid (h2) - sc-406904-KO-2 from Santa Cruz Biotechnology
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Rent3b HDR Plasmid (h2) - sc-406904-HDR-2 from Santa Cruz Biotechnology
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shRNA set against Human UPF3B (NM_023010.3) - SHH440766 from Creative biogene
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UPF3B shRNA Plasmid (Human) - CHJ2182 from Cohesion Biosciences
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UPF3B miRNA 3'UTR clone - MiUTR3H-02691 from Creative biogene
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UPF3B miRNA 3'UTR clone - MiUTR3H-02692 from Creative biogene
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