UPF3B

Gene Symbol UPF3B
Entrez Gene 65109
Alt Symbol HUPF3B, MRX62, MRXS14, RENT3B, UPF3X
Species Human
Gene Type protein-coding
Description UPF3 regulator of nonsense transcripts homolog B (yeast)
Other Description hUpf3p-X|mental retardation, X-linked 62|nonsense mRNA reducing factor 3B|regulator of nonsense transcripts 3B|up-frameshift suppressor 3 homolog B|up-frameshift suppressor 3 homolog on chromosome X
Swissprots Q9H1J0 Q0VAK8 D3DWI3 Q9BZI7 D3DWI4
Accessions AFL55650 EAW89842 EAW89843 EAW89844 EAW89845 EAW89846 Q9BZI7 AF318576 AAG60691 AK093728 BAG52755 AY013251 AAG48511 BC008494 BC121017 AAI21018 BC121018 AAI21019 BF223724 BI549935 CN412717 DA949746 EG328250 XM_005262458 XP_005262515 XM_006724780 XP_006724843 XM_006724781 XP_006724844 XM_011531378 XP_011529680 XM_011531379 XP_011529681 NM_023010 NP_075386 NM_080632 NP_542199
Function Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2- UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF2 stimulates both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA upstream of exon-exon junctions. In vitro, stimulates translation; the function is independent of association with UPF2 and components of the EJC core. {ECO:0000269|PubMed:11163187, ECO:0000269|PubMed:12718880, ECO:0000269|PubMed:16209946, ECO:0000269|PubMed:16601204, ECO:0000269|PubMed:18066079}.
Subcellular Location Nucleus. Cytoplasm. Note=Shuttling between the nucleus and the cytoplasm.
Tissue Specificity Expressed in testis, uterus, prostate, heart, muscle, brain, spinal cord and placenta. {ECO:0000269|PubMed:11113196}.
Top Pathways RNA transport, mRNA surveillance pathway

Rent3 (C-2) - sc-398820 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Rent3 (C-2): sc-398820. Western blot analysis of Rent3b expression in non-transfected 293T: sc-117752 (A), mouse Rent3b transfected 293T: sc-123066 (B), HeLa (C), Jurkat (D) and K-562 (E) whole cell lysates.

Rent3 (E-8) - sc-398821 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Rent3 (E-8): sc-398821. Western blot analysis of Rent3b expression in non-transfected 293T: sc-117752 (A), mouse Rent3b transfected 293T: sc-123066 (B), HeLa (C), Jurkat (D) and K-562 (E) whole cell lysates.

Rent3 (H-190) - sc-48800 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Rent3 (H-190): sc-48800. Western blot analysis of Rent3b expression in non-transfected: sc-117752 (A) and mouse Rent3b transfected: sc-123066 (B) 293T whole cell lysates.

Rent3 (K-16) - sc-20232 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Rent3b (N-17) - sc-20231 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Rent3b (N-17): sc-20231. Western blot analysis of Rent3b expression in K-562 whole cell lysate.

Rent3b (C-17) - sc-20233 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Rent3b (C-17): sc-20233. Western blot analysis of Rent3b expression in non-transfected: sc-117752 (A) and mouse Rent3b transfected: sc-123066 (B) 293T whole cell lysates.

Anti-UPF3B/RENT3B antibody - ab66753 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling UPF3B/RENT3B with ab66753 at 4-8µg/ml. Arrows indicate positively labeling epithelial cells of the fundic gland. Magnification: 400X.

Anti-UPF3B/RENT3B antibody - ab83249 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-UPF3B/RENT3B antibody (ab83249) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal heart lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-UPF3B/RENT3B antibody - ab134566 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-UPF3B/RENT3B antibody (ab134566) at 0.1 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : 293T whole cell lysate at 50 µgLane 4 : Jurkat whole cell lysate at 50 µgdeveloped using the ECL technique

Anti-UPF3B/RENT3B antibody - ab134582 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Detection of UPF3B/RENT3B by Western Blot of Immunprecipitate. Immunoprecipitation of HeLa whole cell lysate using ab134582 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Immunoblot analysis of UPF3B/RENT3B utilised an antibody to an upstream epitope at 1µg/ml. The lysate in lane 2 is precipitated with an IgG control antibody. Detection: Chemiluminescence with exposure time of 3 seconds.

Anti-UPF3B/RENT3B antibody - ab42731 from Abcam

Prices $242.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-UPF3B Antibody (aa300-350) - LS-C290344 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-UPF3B Antibody (aa433-483) - LS-C286180 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-UPF3B Antibody (aa252-301) - LS-C101886 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
UPF3B antibody LS-C101886 Western blot of Fetal Heart lysate.

Anti-UPF3B Antibody (N-Terminus) - LS-C31657 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
UPF3B antibody LS-C31657 was used in IHC to stain formalin-fixed, paraffin-embedded human stomach.

UPF3B Antibody - NBP1-83135 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: UPF3B Antibody [NBP1-83135] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells). Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla).

UPF3B Antibody - NBP1-83134 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: UPF3B Antibody [NBP1-83134] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

UPF3B Antibody - NBP1-57232 from Novus Biologicals

Prices $299.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

UPF3B Antibody - NBP1-57233 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: UPF3B Antibody [NBP1-57233] - Titration: 0.2-1 ug/ml, Positive Control: Human heart.

Anti-UPF3B antibody produced in rabbit - SAB2102656 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-UPF3B: <B>Cat. No.SAB2102656</B>: UPF3B in Fetal Heart tissue lysate with UPF3B antibody at 1.0 μg/mL.

Anti-UPF3B antibody produced in rabbit - HPA001800 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-UPF3B: <B>Cat. No. HPA001800</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human placenta tissue showing strong cytoplasmic and/or membranous staining of trophoblastic cells.

Anti-UPF3B antibody produced in rabbit - HPA001882 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-UPF3B: <B>Cat. No. HPA001882</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic and/or membranous staining of myocytes.

UPF3B antibody - N-terminal region (ARP40998_T100) - ARP40998_T100 from Aviva Systems Biology

Prices $229.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

UPF3B antibody - middle region (ARP41088_P050) - ARP41088_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

UPF3B antibody - 70R-1348 from Fitzgerald

Prices $275.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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