HMBS

Gene Symbol HMBS
Entrez Gene 3145
Alt Symbol PBG-D, PBGD, PORC, UPS
Species Human
Gene Type protein-coding
Description hydroxymethylbilane synthase
Other Description porphobilinogen deaminase|porphyria, acute; Chester type|pre-uroporphyrinogen synthase|uroporphyrinogen I synthase|uroporphyrinogen I synthetase
Swissprots G3V1P4 Q16012 P08396 A8K2L0 G5EA58 P08397
Accessions AAA60028 AAA60029 AAA60030 AAC60602 ABI36594 AHW82961 BAA01463 BAA02216 BAD36778 BAG71787 CAA48156 EAW67447 EAW67448 EAW67449 EAW67450 P08397 AK000628 AK131072 AK290275 BAF82964 AK309544 AK310343 AW139390 BC000520 AAH00520 BC008149 AAH08149 BC019323 AAH19323 BF210364 BQ020524 BX647328 CB128006 D28419 BAA05785 HQ448217 ADQ32697 HQ731552 ADX60069 M60888 X04217 CAA27801 X04808 CAA28499 XM_005271531 XP_005271588 XM_005271532 XP_005271589 XM_005271533 XP_005271590 XM_011542796 XP_011541098 NM_000190 NP_000181 NM_001024382 NP_001019553 NM_001258208 NP_001245137 NM_001258209 NP_001245138
Function Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.
Subcellular Location Cytoplasm {ECO:0000305}.
Tissue Specificity Isoform 1 is ubiquitously expressed. Isoform 2 is found only in erythroid cells. {ECO:0000269|PubMed:3422427}.
Top Pathways Porphyrin and chlorophyll metabolism

PBGD (H-11) - sc-166842 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PBGD (H-11): sc-166842. Western blot analysis of PBGD expression in non-transfected: sc-110760 (A) and human PBGD transfected: sc-110913 (B) 293 whole cell lysates.

PBGD (E-9) - sc-166743 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PBGD (E-9): sc-166743. Western blot analysis of PBGD expression in non-transfected: sc-110760 (A) and human PBGD transfected: sc-110913 (B) 293 whole cell lysates.

PBGD (C-13) - sc-46619 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PBGD (C-13): sc-46619. Western blot analysis of PBGD expression in non-transfected: sc-110760 (A) and human PBGD transfected: sc-110913 (B) 293 whole cell lysates.

PBGD (B-6) - sc-166788 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PBGD (B-6): sc-166788. Western blot analysis of PBGD expression in non-transfected: sc-110760 (A) and human PBGD transfected: sc-110913 (B) 293T whole cell lysates.

PBGD (B-3) - sc-166742 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PBGD (B-3): sc-166742. Western blot analysis of PBGD expression in non-transfected: sc-110760 (A) and mouse PBGD transfected: sc-122406 (B) 293 whole cell lysates.

PBGD (H-300) - sc-67037 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PBGD (H-300): sc-67037. Western blot analysis of PBGD expression in Hep G2 whole cell lysate.

PBGD (A-16) - sc-46618 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PBGD (A-16): sc-46618. Western blot analysis of PBGD expression in non-transfected: sc-110760 (A) and human PBGD transfected: sc-110913 (B) 293 whole cell lysates.

PBGD (I-17) - sc-46621 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PBGD (I-17): sc-46621. Western blot analysis of PBGD expression in non-transfected: sc-110760 (A) and human PBGD transfected: sc-110913 (B) 293 whole cell lysates.

Anti-HMBS antibody - ab185929 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of Immunoprecipitates.Immunoprecipitation of 293T whole cell lysate (1 mg for IP; 20% of IP loaded) using ab185929 at 6 µg/mg lysate for IP and at 1 µg/ml for blottingDetection: Chemiluminescence with an exposure time of 10 seconds.

Anti-HMBS antibody - ab55166 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-HMBS antibody (ab55166) at 5 µg/ml + 0.2 µg/lane of full length recombinant HMBS protein with a tag. SecondaryGoat anti mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

Anti-HMBS antibody - ab91331 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-HMBS antibody (ab91331) at 1/100 dilution + T47D cell line lysate at 35 µg

Anti-HMBS antibody - ab101566 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
ab101566, at a 1/100 dilution, showing cytoplasmic staining of HMBS in a formalin fixed, paraffin embedded section of Human fetal colon by Immunohistochemistry.

Anti-HMBS antibody [1F1] - ab180406 from Abcam

Prices $357.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1F1
All lanes : Anti-HMBS antibody [1F1] (ab180406) at 1/2000 dilutionLane 1 : Non-transfected HEK293T cell lysateLane 2 : HMBS transfected HEK293T cell lysateLysates/proteins at 5 µg per lane.

Anti-HMBS antibody [EPR8105] - ab129092 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8105
All lanes : Anti-HMBS antibody [EPR8105] (ab129092) at 1/1000 dilutionLane 1 : HepG2 cell lysates Lane 2 : HeLa cell lysates Lane 3 : U937 cell lysates Lane 4 : K562 cell lysates Lysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-HMBS / PBGD Antibody (aa1-329) - LS-C311552 from LifeSpan Bioscience

Prices $250.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-HMBS / PBGD Antibody (aa84-352) - LS-C311784 from LifeSpan Bioscience

Prices $250.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-HMBS / PBGD Antibody (N-Terminus) - LS-C81861 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-HMBS / PBGD Antibody (Internal, clone EPR8105) - LS-C152588 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8105
Western blot analysis on (A) HepG2, (B) HeLa, (C) U937, and (D) K562 cell lysates using anti-HMBS antibody.

Anti-HMBS / PBGD Antibody (aa146-195) - LS-C81862 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Fetal liver lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-HMBS / PBGD Antibody - LS-C192793 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts of HeLa cell lines, using HMBS antibody.

Anti-HMBS / PBGD Antibody (clone 1F1) - LS-C336587 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1F1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HMBS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HMBS.

Anti-HMBS / PBGD Antibody (C-Terminus) - LS-C345804 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of HEM3 Antibody with human Fetal Lung lysate.

Anti-HMBS / PBGD Antibody (clone 3E8) - LS-C197420 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3E8
Western Blot analysis of HMBS expression in transfected 293T cell line by HMBS monoclonal antibody (M01), clone 3E8.Lane 1: HMBS transfected lysate(39.71 KDa).Lane 2: Non-transfected lysate.

Anti-HMBS / PBGD Antibody (clone 3E2) - LS-C336651 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3E2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HMBS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HMBS.

Anti-HMBS / PBGD Antibody (clone 3F4) - LS-C336626 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3F4
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HMBS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HMBS.
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