SYMPK

Gene Symbol SYMPK
Entrez Gene 8189
Alt Symbol SPK, SYM
Species Human
Gene Type protein-coding
Description symplekin
Other Description -
Swissprots O00521 Q59GT5 Q92797 Q8N2U5 O00733 O00689
Accessions EAW57389 EAW57390 EAW57391 EAW57392 EAW57393 Q92797 AB209024 BAD92261 AB210010 BAE06092 AF049526 AAC27504 AK130602 AK300252 BAH13243 BC006536 AAH06536 BC006567 AAH06567 BC030214 AAH30214 BU752018 BX649165 DB044963 DB461873 DQ893447 ABM84373 EU176761 ABW03562 U49240 AAC50667 U88726 AAB58578 Y10931 CAA71861 XM_005259286 XP_005259343 XM_011527354 XP_011525656 XM_011527355 XP_011525657 XR_243962 NM_004819 NP_004810
Function Scaffold protein that functions as a component of a multimolecular complex involved in histone mRNA 3'-end processing. Specific component of the tight junction (TJ) plaque, but might not be an exclusively junctional component. May have a house- keeping rule. Is involved in pre-mRNA polyadenylation. Enhances SSU72 phosphatase activity. {ECO:0000269|PubMed:16230528, ECO:0000269|PubMed:20861839}.
Subcellular Location Cytoplasm, cytoskeleton. Cell junction, tight junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction. Nucleus, nucleoplasm. Note=Cytoplasmic face of adhesion plaques (major) and nucleoplasm (minor) (in cells with TJ). Nucleoplasm (in cells without TJ). Nuclear bodies of heat-stressed cells.
Tissue Specificity In testis, expressed in polar epithelia and Sertoli cells but not in vascular endothelia. The protein is detected in stomach, duodenum, pancreas, liver, fetal brain, carcinomas, lens-forming cells, fibroblasts, lymphocytes, lymphoma cells, erythroleukemia cells but not in endothelium of vessels, epidermis, intercalated disks, Purkinje fiber cells of the heart and lymph node. {ECO:0000269|PubMed:8769423}.
Top Pathways Tight junction, mRNA surveillance pathway

Symplekin (D5Y3T) Rabbit mAb - 13071 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5Y3T
Western blot analysis of extracts from various cell lines using Symplekin (D5Y3T) Rabbit mAb.

Symplekin Antibody - 9981 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

symplekin (E-2) - sc-514260 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
symplekin (E-2): sc-514260. Western blot analysis of symplekin expression in COLO 320DM (A), Jurkat (B) and K-562 (C) whole cell lysates.

symplekin (G-6) - sc-398897 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
symplekin (G-6): sc-398897. Western blot analysis of symplekin expression in non-transfected: sc-117752 (A) and human symplekin transfected: sc-114051 (B) 293T whole cell lysates.

symplekin (H-300) - sc-135410 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
symplekin (H-300): sc-135410. Western blot analysis of symplekin expression in Jurkat (A), K-562 (B) and Caco-2 (C) whole cell lysates.

symplekin (S-16) - sc-240937 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
symplekin (S-16): sc-240937. Western blot analysis of symplekin expression in non-transfected: sc-117752 (A) and human symplekin transfected: sc-114051 (B) 293T whole cell lysates.

symplekin (YI-9) - sc-101128 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
symplekin (YI-9): sc-101128. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human ovary, clear cell carcinoma tissue showing nuclear localization.

Anti-Symplekin antibody - ab168638 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-Symplekin antibody (ab168638) at 1/500 dilutionLane 1 : Symplekin transfected 293T cell lysate Lane 2 : Non-transfected 293T cell line lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilutiondeveloped using the ECL technique

Anti-Symplekin antibody - ab187018 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-Symplekin antibody - ab84126 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Sample Type : Human HT29 cells. Primary Antibody Dilution : 1:50. Secondary Antibody : Donkey anti Rabbit-Alexa Fluor 488. Secondary Antibody Dilution : 1:500. Color/Signal Descriptions : Green: Symplekin Blue: DAPI. Gene Name : SYMPK.

Anti-Symplekin antibody - ab56453 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Symplekin antibody (ab56453) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human ovary, clear cell carcinoma.

Anti-Symplekin antibody - ab80274 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Symplekin antibody (ab80274) at 0.4 µg/mlLane 1 : Whole cell lysate from HeLa cells at 50 µgLane 2 : Whole cell lysate from HeLa cells at 15 µgLane 3 : Whole cell lysate from HeLa cells at 5 µg

Anti-Symplekin antibody - ab99257 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab99257 at 1/250 dilution staining Symplekin in formalin-fixed, paraffin-embedded section of Human colon carcinoma by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Detection utilised DAB.

Anti-Symplekin antibody - ab80273 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SYMPK / Symplekin Antibody - LS-C124626 from LifeSpan Bioscience

Prices $560.00
Sizes 5 ml
Host Mouse
Clonality Monoclonal

Anti-SYMPK / Symplekin Antibody (clone Sym-TJ-E150) - LS-C193422 from LifeSpan Bioscience

Prices $415.00
Sizes 5 ml
Host Mouse
Clonality Monoclonal
Clone Sym-TJ-E150

Anti-SYMPK / Symplekin Antibody (aa200-250) - LS-C288258 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SYMPK / Symplekin Antibody (aa350-400) - LS-C288259 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SYMPK / Symplekin Antibody (aa1-534, Azide-free) - LS-C328816 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-SYMPK / Symplekin Antibody (aa180-229) - LS-C101988 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
SYMPK / Symplekin antibody LS-C101988 Western blot of Fetal Liver lysate.

Anti-SYMPK / Symplekin Antibody (C-Terminus) - LS-C110726 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
SYMPK / Symplekin antibody LS-C110726 Western blot of HepG2 cell lysate.

Anti-SYMPK / Symplekin Antibody (aa1224-1274) - LS-C288261 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human Symplekin by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Symplekin used at a dilution of 1:200 (1 ug/ml). Detection: DAB.

Anti-SYMPK / Symplekin Antibody (aa1224-1274) - LS-C286744 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human Symplekin Immunohistochemistry. Sample: FFPE section of human pancreatic islet cell tumor. Antibody: Affinity purified rabbit anti-Symplekin used at a dilution of 1:250.

Anti-SYMPK / Symplekin Antibody (clone 4C2) IHC-plus™ - LS-B6171 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 4C2
Anti-SYMPK / Symplekin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6171 concentration 5 ug/ml.

Symplekin Antibody (4C2) - H00008189-M03 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4C2
Western Blot: Symplekin Antibody (4C2) [H00008189-M03] - Analysis of SYMPK expression in transfected 293T cell line by SYMPK monoclonal antibody (M03), clone 4C2. Lane 1: SYMPK transfected lysatE (58.9 KDa). Lane 2: Non-transfected lysate.
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