NDST1

Gene Symbol NDST1
Entrez Gene 3340
Alt Symbol HSST, MRT46, NST1
Species Human
Gene Type protein-coding
Description N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1
Other Description HSNST 1|N-HSST 1|N-heparan sulfate sulfotransferase 1|NDST-1|[Heparan sulfate]-glucosamine N-sulfotransferase 1|bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1|glucosaminyl N-deacetylase/N-sulfotransferase 1|heparan sulfate-N-deacetylase/N-sulfotransferase
Swissprots Q96E57 P52848
Accessions EAW61720 EAW61721 EAW61722 P52848 AB209107 BAD92344 AK292448 BAF85137 AK293746 BAH11586 BC012888 AAH12888 BC015828 ES315645 U17970 AAA67765 U18918 AAA75281 U18932 U36600 AAC27354 XM_005268433 XP_005268490 XM_005268434 XP_005268491 XM_005268435 XP_005268492 XM_005268436 XP_005268493 XM_005268437 XP_005268494 XM_005268438 XP_005268495 XM_005268439 XP_005268496 XM_005268441 XP_005268498 XM_005268442 XP_005268499 XM_006714782 XP_006714845 XM_006714783 XP_006714846 XM_011537638 XP_011535940 XR_245854 NM_001301063 NP_001287992 NM_001543 NP_001534
Function Essential bifunctional enzyme that catalyzes both the N- deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L- selectin ligands, thereby playing a role in inflammatory response. {ECO:0000269|PubMed:10758005, ECO:0000269|PubMed:12634318}.
Subcellular Location Golgi apparatus membrane {ECO:0000269|PubMed:9230113}; Single-pass type II membrane protein {ECO:0000269|PubMed:9230113}.
Tissue Specificity Widely expressed. Expression is most abundant in heart, liver and pancreas.
Top Pathways Glycosaminoglycan biosynthesis - heparan sulfate / heparin

NDST (E-9) - sc-374529 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NDST (E-9): sc-374529. Western blot analysis of NDST expression in A549 (A) and ES-2 (B) whole cell lysates.

NDST (H-300) - sc-33589 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

NDST1 (A-20) - sc-16762 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NDST1 (A-20): sc-16762. Western blot analysis of NDST1 expression in JAR (A), A549 (B), Jurkat (C), Hep G2 (D) and PANC-1 (E) whole cell lysates.

NDST1 (N-20) - sc-16761 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

NDST1 (FF-2) - sc-100790 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
NDST1 (FF-2): sc-100790. Western blot analysis of NDST1 expression in A549 whole cell lysate.

Anti-NDST1 antibody - ab55296 from Abcam

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Overlay histogram showing HepG2 cells stained with ab55296 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55296, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-NDST1 antibody - ab129248 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-NDST1 antibody (ab129248) at 1/100 dilution + CEM cell line lysates at 35 µg

Anti-NDST1 antibody - ab176908 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-NDST1 antibody (ab176908) at 1/200 dilution + A549 lysate

Anti-NDST1 Antibody (aa820-849, AP) - LS-C264135 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NDST1 Antibody (aa820-849, APC) - LS-C264137 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NDST1 Antibody (aa820-849, Biotin) - LS-C264138 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NDST1 Antibody (aa820-849, FITC) - LS-C264139 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NDST1 Antibody (aa820-849, Azide-free, HRP) - LS-C264140 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NDST1 Antibody (aa820-849, PE) - LS-C264141 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NDST1 Antibody (aa819-849) - LS-C323261 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NDST1 Antibody (aa820-849) - LS-C164792 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
NDST1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-NDST1 Antibody (clone 1G10) - LS-C133263 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1G10
Immunoperoxidase of monoclonal antibody to NDST1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]

N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10) - H00003340-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1G10
Western Blot: N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10) [H00003340-M01] - NDST1 monoclonal antibody (M01), clone 1G10 Analysis of NDST1 expression in A-549.

N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody - NBP2-37978 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry: N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody [NBP2-37978] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.

N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (2F11) - H00003340-M03 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2F11
Western Blot: N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (2F11) [H00003340-M03] - Analysis of NDST1 expression in A-549 (Cat # L025V1).

N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody - H00003340-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10) - H00003340-M01A from Novus Biologicals

Host Mouse
Clonality Monoclonal
Clone 1G10

NDST1 Polyclonal Antibody - bs-9891R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Monoclonal Anti-NDST1 antibody produced in mouse - SAB1403952 from Sigma-Aldrich

Prices $347.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone 1G10
<B>Immunoblotting</B><BR/>Monoclonal Anti-NDST1: <B>Cat. No. SAB1403952</B>: Antibody concentration: 1/500x dilution. Specific band of ~100.9 kDa using A~549 lysate.

Monoclonal Anti-NDST1 antibody produced in mouse - WH0003340M1 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1G10
<B>Immunoblotting</B><BR/>Monoclonal Anti-NDST1, <B>Cat. No. WH0003340M1</B> used at the antibody concentration: 1 ~ 5 μg/mL. Specific band of ~100.8 kDa using A-549 lysate.
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