NFATC2

Gene Symbol NFATC2
Entrez Gene 4773
Alt Symbol NFAT1, NFATP
Species Human
Gene Type protein-coding
Description nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
Other Description NF-ATc2|NFAT pre-existing subunit|NFAT transcription complex, preexisting component|T cell transcription factor NFAT1|T-cell transcription factor NFAT1|nuclear factor of activated T-cells, cytoplasmic 2|nuclear factor of activated T-cells, preexisting component|preexisting nuclear factor of activated T-cells 2
Swissprots Q9UJR2 B5B2P2 Q13469 B5B2N8 Q9NQH3 Q13468 Q5TFW7 B5B2N9 B5B2P0 B5B2P3 Q9NPX6 Q5TFW8
Accessions EAW75602 EAW75603 Q13469 AK025758 AK226146 AK304147 BAH14117 AY927566 BC136418 AAI36419 BC144074 AAI44075 BE674599 BF914609 BM463165 BU429325 CD300621 CF136470 CR993438 EU887573 ACG55593 EU887574 ACG55594 EU887575 ACG55595 EU887576 ACG55596 EU887577 ACG55597 EU887578 ACG55598 EU887579 ACG55599 EU887580 ACG55600 U43341 AAC50886 U43342 AAC50887 Z36756 XM_011528824 XP_011527126 XM_011528825 XP_011527127 XM_011528826 XP_011527128 NM_001136021 NP_001129493 NM_001258292 NP_001245221 NM_001258294 NP_001245223 NM_001258295 NP_001245224 NM_001258296 NP_001245225 NM_001258297 NP_001245226 NM_012340 NP_036472 NM_173091 NP_775114
Function Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway. {ECO:0000269|PubMed:15790681, ECO:0000269|PubMed:21871017}.
Subcellular Location Cytoplasm. Nucleus. Note=Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
Tissue Specificity Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas. Isoform 1 is highly expressed in the small intestine, heart, testis, prostate, thymus, placenta and thyroid. Isoform 3 is highly expressed in stomach, uterus, placenta, trachea and thyroid. {ECO:0000269|PubMed:8668213}.
Top Pathways Wnt signaling pathway, Osteoclast differentiation, B cell receptor signaling pathway, HTLV-I infection, T cell receptor signaling pathway

NFAT1 (D43B1) XP ® Rabbit mAb (PE Conjugate) - 14335 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D43B1
Flow cytometric analysis of LNCaP (blue) and Jurkat (green) cells using NFAT1 (D43B1) XP ® Rabbit mAb (PE Conjugate).

NFAT1 (D43B1) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 14324 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D43B1
Flow cytometric analysis of LNCap (Blue) and Jurkat cells (green) using NFAT1 (D43B1) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate).

NFAT1 (D43B1) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 14201 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D43B1
Flow cytometric analysis of LNCap (blue) and Jurkat cells (green) using NFAT1 (D43B1) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate).

NFAT1 (D43B1) XP ® Rabbit mAb - 5861 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D43B1
Western blot analysis of extracts from Ramos, Raji, and EL4 cells using NFAT1 (D43B1) XP ® Rabbit mAb.

NFAT1 (D9C2) Rabbit mAb - 5862 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D9C2
Western blot analysis of extracts from Ramos, Raji, and EL4 cells using NFAT1 (D9C2) Rabbit mAb.

NFAT1 Antibody - 4389 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using NFAT1 Antibody.

NFATc2 (4G6-G5) - sc-7296 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NFATc2 (4G6-G5): sc-7296. Western blot analysis of NFATc2 expression in IL-2-induced Jurkat whole cell lysate.

NFATc2 (G1-D10) - sc-7295 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of NFATc2 expression in Ramos whole cell lysate immunoprecipitated with NFATc2 (G1-D10): sc-7295 and detected with NFATc2 (4G6-G5): sc-7296.

NFATc2 (G-20) - sc-23815 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

NFATc2 (M-300) - sc-13034 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NFATc2 (M-300): sc-13034. Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic and nuclear staining of cells in seminiferous ducts.

NFATc2 (1) - sc-136206 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
NFATc2 (1): sc-136206. Western blot analysis of NFATc2 expression in Jurkat whole cell lysate.

p-NFATc2 (Ser 175) - sc-68695 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

p-NFATc2 (Ser 326) - sc-32994 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
p-NFATc2 (Ser 326): sc-32994. Western blot analysis of NFATc2 phosphorylation in untreated (A,C) and IL-2-treated (B,D) Jurkat whole cell lysates. Antibody was preincubated with cognate phosphorylated peptide in blots (C) and (D).

p-NFATc2 (Ser 213/217/221) - sc-32991 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
p-NFATc2 (Ser 213/217/221): sc-32991. Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing cytoplasmic staining of cells in non-germinal center.

p-NFATc2 (Ser 243) - sc-32993 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

p-NFATc2 (Ser 268) - sc-68697 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

p-NFATc2 (Ser 274) - sc-68698 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

p-NFATc2 (Ser 276) - sc-68699 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

p-NFATc2 (Ser 280) - sc-68700 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

p-NFATc2 (Ser 177) - sc-68696 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-NFAT1 antibody - ab49161 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-NFAT1 antibody (ab49161) at 1.25 µg/ml + SP2/0 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50,000 - 1/100,000 dilution

Anti-NFAT1 antibody - ab95479 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-NFAT1 antibody (ab95479) at 0.3 µg/ml + MOLT4 lysate at 35 µgdeveloped using the ECL technique

Anti-NFAT1 antibody - ab46955 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-NFAT1 antibody [25A10.D6.D2] - ChIP Grade - ab2722 from Abcam

Prices $403.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 25A10.D6.D2
ab2722 (4µg/ml) staining NFAT in human tonsil, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear and weak cytoplasmic staining.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-NFAT1 antibody [EPR2973] - ab92490 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2973
All lanes : Anti-NFAT1 antibody [EPR2973] (ab92490) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : Ramos cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
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