AP1B1

Gene Symbol AP1B1
Entrez Gene 162
Alt Symbol ADTB1, AP105A, BAM22, CLAPB2
Species Human
Gene Type protein-coding
Description adaptor-related protein complex 1, beta 1 subunit
Other Description AP-1 complex subunit beta-1|Golgi adaptor HA1/AP1 adaptin beta subunit|adapter-related protein complex 1 subunit beta-1|adaptor protein complex AP-1 subunit beta-1|adaptor-related protein complex 1 subunit beta-1|beta-1-adaptin|beta-adaptin 1|beta-prime-adaptin|beta1-adaptin|clathrin assembly protein complex 1 beta large chain|plasma membrane adaptor HA2/AP2 adaptor beta subunit
Swissprots Q86X54 Q20WL3 P78436 F8WDL0 C9JRD1 Q10567
Accessions AAC50684 CBF79614 CBU99115 EAW59798 EAW59799 EAW59800 Q10567 AI084003 AK294406 BAG57658 AK296567 BAG59187 AK299613 BAG61541 AU139022 BC046242 AAH46242 BG999878 BM909530 BX537680 CAD97809 CD364485 CT841508 CAJ86438 CU012936 CAK54367 CU013224 CAK54666 L13939 AAC98702 NM_001127 NP_001118 NM_001166019 NP_001159491 NM_145730 NP_663782
Function Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Subcellular Location Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
Tissue Specificity Widely expressed.
Top Pathways Lysosome

β-Adaptin (H-300) - sc-10762 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
β-Adaptin (H-300): sc-10762. Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.

β-Adaptin (N-19) - sc-6425 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
β-Adaptin (N-19): sc-6425. Western blot analysis of β-Adaptin expression in HeLa (A), A-431 (B), K-562 (C) and NIH/3T3 (D) whole cell lysates.

β-Adaptin (A-5) - sc-74423 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
β-Adaptin (A-5): sc-74423. Western blot analysis of β-Adaptin expression in IMR-32 (A) and HeLa (B) whole cell lysates.

Anti-AP1+2 beta antibody - ab21981 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-Clathrin antibody - ab59710 from Abcam

Prices $388.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab59710 at 1/1000, overnight, secondary antibody biotinylated goat anti-sheep at 1/3000.Figure A is a low power image showing intense staining of non-ciliated bronchiolar cells (Clara cells) and lesser staining in alveolar type II cells.Figure B is a higher power image of the staining of the non ciliated bronchiolar cells.

Anti-Clathrin antibody [CHC5.9] - ab35571 from Abcam

Prices $372.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone CHC5.9

Anti-Clathrin antibody [X22] - ab2731 from Abcam

Prices $403.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone X22
Immunocytochemistry/Immunofluorescence analysis of Clathrin shows staining in HeLa cells. Clathrin, Heavy chain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2731 (1:200) over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse secondary antibody. Images were taken at 60X magnification.

Anti-AP1B1 / BAM22 Antibody (aa490-539) - LS-C135683 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
AP1B1 antibody LS-C135683 Western blot of Fetal Muscle lysate.

Anti-AP1B1 / BAM22 Antibody (aa11-29) - LS-C69067 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

ADTB1 Antibody - NBP1-68947 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: ADTB1 Antibody [NBP1-68947] - ADTB1 Antibody Mouse WT brain and Rat brain, concentration 2ug/ml.

Anti-AP1B1 antibody produced in rabbit - SAB2700240 from Sigma-Aldrich

Prices $326.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-AP1B1: <B>Cat. No. SAB2700240</B>: Western Blot analysis of AP1B1 in extracts from <B>Lanes</B><BR/>A. MOLT4 using <B>Cat. No. SAB2700240</B> at a 1:500 dilution.

Monoclonal Anti-β1 and β2-Adaptins antibody produced in mouse - A4450 from Sigma-Aldrich

Prices $340.00, $551.00
Sizes 200 µl, 500 µl
Host Mouse
Clonality Monoclonal
Clone 100/1
<B>Immunofluorescence</B><BR/>Immunofluorescent staining of 3T3 fibroblast with Monoclonal Anti-β1 and-β2 Adaptins (AP-1 & AP-2, Clone no. 100/1) (<B>Cat. No. A4450</B>).<BR/>From E. Ungewickell, Washington Univ. Sch. of Medicine, St. Louis, MO.

AP1B1 antibody - C-terminal region (ARP59977_P050) - ARP59977_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

ap1b1 antibody - zf125569 from GeneTex

Host Rabbit
Clonality Polyclonal

ap1b1 antibody - zf125570 from GeneTex

Host Rabbit
Clonality Polyclonal

Clathrin antibody - GTX26316 from GeneTex

Prices $259.00
Sizes 200 μl
Host Goat
Clonality Polyclonal

AP1B1 antibody - 70R-9955 from Fitzgerald

Prices $345.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

AP1B1 antibody - 70R-15745 from Fitzgerald

Prices $375.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

beta Adaptin antibody - 70R-12605 from Fitzgerald

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

AP1B1 antibody (OAGA00120) - OAGA00120 from Aviva Systems Biology

Prices $340.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-Clathrin Antibody - 11-526 from Abcore

Host Mouse
Clonality Monoclonal

AP1B1 antibody - 16932-1-AP from Acris Antibodies

Prices $270.00
Sizes 150 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of HepG2 cells, using AP1B1 antibody 16932-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

AP1 complex subunit beta-1 / AP1B1 (C-term) antibody - AP45984PU-N from Acris Antibodies

Prices $340.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Human Muscle; WB Suggested Anti-AP1B1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Muscle; AP1B1 antibody - C-terminal region (AP45984PU-N) in Human Muscle cells using Western Blot

AP1 complex subunit beta-1 / AP1B1 antibody - PAB14335 from Acris Antibodies

Prices $420.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western blot analysis on human brain lysate under reducing conditions using AP1B1 polyclonal antibody ( Cat # PAB14335 ) at a dilution of 1 : 100. This antibody labels a major protein of approximately 100 kDa of molecular weight and several minor proteins around 70 kDa in both rat and human tissue. The presence of the two lower bands ( IgG fragments ) is due to the magnetic bead purification method employed.
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