NCBP2

Gene Symbol NCBP2
Entrez Gene 22916
Alt Symbol CBC2, CBP20, NIP1, PIG55
Species Human
Gene Type protein-coding
Description nuclear cap binding protein subunit 2, 20kDa
Other Description 20 kDa nuclear cap-binding protein|NCBP 20 kDa subunit|NCBP interacting protein 1|NCBP-interacting protein 1|cell proliferation-inducing gene 55 protein|nuclear cap binding protein subunit 2, 20kD|nuclear cap-binding protein subunit 2
Swissprots B2RE91 B4DMK7 Q14924 P52298 Q2TS50 E9PAR5
Accessions EAW53620 EAW53621 EAW53622 EAW53623 EAW53624 P52298 AK093216 BAG52672 AK296144 BAG58884 AK297506 BAG59919 AK308192 AK315903 BAH14274 AK316601 BAG38188 AL110262 AW440347 AY644767 AAV85455 BC001255 AAH01255 BP248419 BT006842 AAP35488 BX537372 CAD97614 BX647994 CN421262 D59253 BAA09599 DC375323 DQ890703 ABM81629 DQ893887 ABM84813 X84157 CAA58962 XM_005269313 XP_005269370 XM_011512556 XP_011510858 XM_011512557 XP_011510859 XM_011512558 XP_011510860 NM_001042540 NP_001036005 NM_001308036 NP_001294965 NM_007362 NP_031388
Function Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5' end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC com
Subcellular Location Nucleus {ECO:0000269|PubMed:8601613}. Cytoplasm {ECO:0000269|PubMed:8601613}.
Top Pathways Spliceosome, RNA transport, mRNA surveillance pathway

CBP20 (B-1) - sc-137123 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CBP20 (B-1): sc-137123. Western blot analysis of CBP20 expression in non-transfected 293: sc-110760 (A), human CBP20 transfected 293: sc-110845 (B) and Ca Ski (C) whole cell lysates and HeLa (D) and SK-BR-3 (E) nuclear extracts.

CBP20 (FL-156) - sc-48793 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
CBP20 (FL-156): sc-48793. Western blot analysis of CBP20 expression in non-transfected 293: sc-110760 (A) and human CBP20 transfected 293: sc-110845 (B) whole cell lysates and HeLa (C) and SK-BR-3 (D) nuclear extracts.

CBP20 (T-15) - sc-23627 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-NCBP2 antibody - ab91560 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling NCBP2 with ab91560 at 1/5000 (0.2µg/ml) and 1/1000 (1 µg/ml). Detection: DAB.

Anti-NCBP2 antibody - ab124632 from Abcam

Prices $428.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab124632, at 1/100 dilution, staining NCBP2 in formalin-fixed, paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry with DAB staining.

Anti-NCBP2 antibody - ab91556 from Abcam

Prices $370.00
Sizes 10 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling NCBP2 with ab91556 at 1/5000 (0.2µg/ml). Detection: DAB.

Anti-NCBP2 antibody - ab71472 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NCBP2 antibody (ab71472) at 1/60 dilutionLane 1 : CEM cell line lysateLane 2 : Ramos cell line lysateLysates/proteins at 35 µg per lane.

Anti-NCBP2 antibody - ab65126 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NCBP2 antibody (ab65126) at 1/500 dilutionLane 1 : COLO205 cell extractLane 2 : COLO205 cell extract with immunising peptide at 5 µgLysates/proteins at 5 µg per lane.

Anti-NCBP2 antibody [2613C3a] - ab84854 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2613C3a
Anti-NCBP2 antibody [2613C3a] (ab84854) + Immunogen (Recombinant Human NCBP2 fragment)

Anti-NCBP2 / CBP20 Antibody (C-Terminus) - LS-C125988 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-NCBP2 / CBP20 Antibody (clone 2613C3a) - LS-C184731 from LifeSpan Bioscience

Prices $335.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2613C3a

Anti-NCBP2 / CBP20 Antibody (aa10-59) - LS-C118927 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from COLO205 cells, using NCBP2 Antibody. The lane on the right is treated with the synthesized peptide.

Anti-NCBP2 / CBP20 Antibody (Arg21) - LS-C176776 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of CBP20 (R21) pAb in extracts from COLO205 cells.

Anti-NCBP2 / CBP20 Antibody - LS-C346336 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence blot of MCF7 cell using NCBP2 antibody. Blue: DAPI for nuclear staining.

Anti-NCBP2 / CBP20 Antibody (clone 2613C3a) - LS-C342759 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2613C3a
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-NCBP2 mAb.

Anti-NCBP2 / CBP20 Antibody (aa1-50) - LS-C289284 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human NCBP2 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-NCBP2 used at a dilution of 1:5000 (0.2 ug/ml). Detection: DAB.

Anti-NCBP2 / CBP20 Antibody (aa106-156) - LS-C289286 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human and Mouse NCBP2 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-NCBP2 used at a dilution of 1:5000 (0.2 ug/ml) and 1:1000 (1 ug/ml). Detection: DAB.

Anti-NCBP2 / CBP20 Antibody (aa106-156) - LS-C286912 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Mouse NCBP2 by Immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-NCBP2 used at a dilution of 1:100.

CBP20 Antibody - NBP1-91755 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: CBP20 Antibody [NBP1-91755] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

CBP20 Antibody - NBP1-30445 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry: CBP20 Antibody [NBP1-30445] - Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- NCBP2 used at a dilution of 1:5,000 (0.2ug/ml) and 1:1,000 (1 ug/ml). Detection: DAB

CBP20 Antibody - NBP2-15732 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: CBP20 Antibody [NBP2-15732] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.

CBP20 Antibody (3A12) - H00022916-M02 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3A12
Sandwich ELISA: CBP20 Antibody (3A12) [H00022916-M02] - Detection limit for recombinant GST tagged NCBP2 is 0.3 ng/ml as a capture antibody.

Anti-NCBP2 (C-term) antibody produced in rabbit - SAB1300600 from Sigma-Aldrich

Prices $291.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-NCBP2 (C-term) Antibody: <B>Cat. No. SAB1300600</B>: NCBP2 in HeLa and SK-BR-3 cell line lysates with Anti-NCBP2 (C-term) Antibody at 4.0 μg/mL.

Anti-NCBP2 (16-30) antibody produced in rabbit - SAB1101611 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-NCBP2 (16-30) (RB): <B>Cat. No. SAB1101611</B>: Immunofluorescence of HUVEC cells using Anti-NCBP2 (16-30) (RB) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Anti-NCBP2 antibody produced in rabbit - HPA044850 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-NCBP2: <B>Cat. No. HPA044850</B>: Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferus ducts and Leydig cells.
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