| Gene Symbol | KIAA0196 |
|---|---|
| Entrez Gene | 9897 |
| Alt Symbol | RTSC, SPG8 |
| Species | Human |
| Gene Type | protein-coding |
| Description | KIAA0196 |
| Other Description | WASH complex subunit strumpellin|strumpellin |
| Swissprots | Q8TBQ2 A8K4R7 Q12768 Q3KQX5 |
| Accessions | EAW92080 EAW92081 EAW92082 Q12768 AI266595 AK223628 BAD97348 AK291032 BAF83721 AK296970 BAG59514 BC026951 AAH26951 BC106015 AAI06016 D83780 BAA12109 DA185885 XM_005251120 XP_005251177 XM_011517409 XP_011515711 XM_011517410 XP_011515712 NM_014846 NP_055661 |
| Function | Acts at least in part as component of the WASH core complex whose assembly at the surface of endosomes seems to inhibit WASH nucleation-promoting factor (NPF) activity in recruiting and activating the Arp2/3 complex to induce actin polymerization, and which is involved in regulation of the fission of tubules that serve as transport intermediates during endosome sorting (PubMed:19922875, PubMed:20498093). May be involved in axonal outgrowth. Involved in cellular localization of ADRB2 (PubMed:23085491). Involved in cellular trafficking of BLOC-1 complex cargos such as ATP7A and VAMP7 (PubMed:23676666). {ECO:0000269|PubMed:19922875, ECO:0000269|PubMed:20833645, ECO:0000269|PubMed:23085491, ECO:0000269|PubMed:23676666}. |
| Subcellular Location | Cytoplasm, cytosol {ECO:0000269|PubMed:20833645}. Endoplasmic reticulum {ECO:0000269|PubMed:20833645}. Early endosome {ECO:0000269|PubMed:23085491, ECO:0000269|PubMed:23676666}. Note=Colocalizes with SYP/synaptophysin in the external molecular layer of the dentate gyrus and in motoneurons of the ventral horn of spinal cord. {ECO:0000269|PubMed:20833645}. |
| Tissue Specificity | Expressed ubiquitously. {ECO:0000269|PubMed:20833645}. |
Strumpellin CRISPR/Cas9 KO Plasmid (h) - sc-402808 from Santa Cruz Biotechnology
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Strumpellin HDR Plasmid (h) - sc-402808-HDR from Santa Cruz Biotechnology
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Strumpellin Double Nickase Plasmid (h) - sc-402808-NIC from Santa Cruz Biotechnology
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Strumpellin Double Nickase Plasmid (h2) - sc-402808-NIC-2 from Santa Cruz Biotechnology
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Strumpellin CRISPR Activation Plasmid (h) - sc-402808-ACT from Santa Cruz Biotechnology
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Strumpellin CRISPR Activation Plasmid (h2) - sc-402808-ACT-2 from Santa Cruz Biotechnology
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Strumpellin Lentiviral Activation Particles (h) - sc-402808-LAC from Santa Cruz Biotechnology
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Strumpellin Lentiviral Activation Particles (h2) - sc-402808-LAC-2 from Santa Cruz Biotechnology
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Strumpellin siRNA (h) - sc-77748 from Santa Cruz Biotechnology
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Strumpellin shRNA Plasmid (h) - sc-77748-SH from Santa Cruz Biotechnology
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Strumpellin shRNA (h) Lentiviral Particles - sc-77748-V from Santa Cruz Biotechnology
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CompoZr® Knockout ZFN Kit - CKOZFN11064 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND11064 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR03378 from Sigma-Aldrich
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KIAA0196 siRNA (Human) - CRH6642 from Cohesion Biosciences
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Strumpellin CRISPR/Cas9 KO Plasmid (h2) - sc-402808-KO-2 from Santa Cruz Biotechnology
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Strumpellin HDR Plasmid (h2) - sc-402808-HDR-2 from Santa Cruz Biotechnology
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shRNA set against Human KIAA0196 (NM_014846.3) - SHH324507 from Creative biogene
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KIAA0196 shRNA Plasmid (Human) - CHH6642 from Cohesion Biosciences
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KIAA0196 miRNA 3'UTR clone - MiUTR1H-05203 from Creative biogene
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